Escherichia phage phiKT

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Ermolevavirus; Escherichia virus PhiKT

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6VUC0|H6VUC0_9CAUD Uncharacterized protein OS=Escherichia phage phiKT OX=1141519 GN=phiKT_00025 PE=4 SV=1
MM1 pKa = 8.03AITFYY6 pKa = 10.48ATNNFTGDD14 pKa = 3.27GTTVNWNINFADD26 pKa = 4.97GYY28 pKa = 10.67IDD30 pKa = 3.54TTDD33 pKa = 2.79VKK35 pKa = 11.02ARR37 pKa = 11.84YY38 pKa = 9.24LDD40 pKa = 3.62NTGSYY45 pKa = 10.68VDD47 pKa = 3.62IAISSVAGNVVTISPAVADD66 pKa = 3.99GQEE69 pKa = 3.81FQIYY73 pKa = 10.01RR74 pKa = 11.84DD75 pKa = 3.64TEE77 pKa = 3.86KK78 pKa = 10.73RR79 pKa = 11.84FPLVDD84 pKa = 4.55FSDD87 pKa = 4.14GAILNEE93 pKa = 4.32TNLDD97 pKa = 3.78TLATQAVMVSAEE109 pKa = 5.25AFDD112 pKa = 3.66QSNNGVRR119 pKa = 11.84IAGDD123 pKa = 3.59SLTIAQGIDD132 pKa = 2.95AKK134 pKa = 11.17AQLALDD140 pKa = 3.7NSEE143 pKa = 4.45AAVATANGIDD153 pKa = 4.02GKK155 pKa = 10.93AQTALNTAAAAVATANAATSTAAGAVSTANTALSTANDD193 pKa = 4.0AADD196 pKa = 4.1DD197 pKa = 3.97AATAVTTANAAVTTANAATTTANAASAAASAATTTANNAANDD239 pKa = 3.57AATAVSTANTALSTANTASTNASTAVSTANAAKK272 pKa = 9.04ATAEE276 pKa = 4.5GIEE279 pKa = 4.14AKK281 pKa = 10.44ADD283 pKa = 3.45SALAASANAVTTANAAEE300 pKa = 4.13ATANAIDD307 pKa = 4.53GKK309 pKa = 10.17ATDD312 pKa = 4.46ALNTADD318 pKa = 4.59AAEE321 pKa = 4.33ATANTAAATASSAAADD337 pKa = 3.49ASTALSTVTAADD349 pKa = 3.75TKK351 pKa = 11.29AQTALDD357 pKa = 4.44NIASLDD363 pKa = 3.49AATMKK368 pKa = 10.53KK369 pKa = 10.04AANLSDD375 pKa = 3.59IADD378 pKa = 4.07KK379 pKa = 10.83AAAWLNVRR387 pKa = 11.84PIGSTPLAGDD397 pKa = 3.32PVGDD401 pKa = 3.51YY402 pKa = 11.38DD403 pKa = 4.84AVTKK407 pKa = 10.42RR408 pKa = 11.84WVEE411 pKa = 3.74NLINTGTVGPTMNGVMNYY429 pKa = 10.14GVGDD433 pKa = 3.36FHH435 pKa = 8.87LRR437 pKa = 11.84DD438 pKa = 3.4SRR440 pKa = 11.84AYY442 pKa = 7.64IQPYY446 pKa = 9.33EE447 pKa = 4.22VVSDD451 pKa = 3.9GQLLNRR457 pKa = 11.84ADD459 pKa = 3.96WPEE462 pKa = 3.82LWSYY466 pKa = 11.67AQMLSPITDD475 pKa = 4.18ADD477 pKa = 3.72WLADD481 pKa = 3.52PQKK484 pKa = 10.32RR485 pKa = 11.84GKK487 pKa = 10.55YY488 pKa = 9.69SLGNGATTFRR498 pKa = 11.84VPDD501 pKa = 3.77RR502 pKa = 11.84NGVQAGSIAALFGRR516 pKa = 11.84GDD518 pKa = 3.85GGVSSSDD525 pKa = 3.19GTILDD530 pKa = 3.74SAAPNITGSFGRR542 pKa = 11.84LVYY545 pKa = 10.79ASTGTIYY552 pKa = 10.59EE553 pKa = 4.57GNTGTGAFSAFSPQAKK569 pKa = 7.99YY570 pKa = 11.16KK571 pKa = 10.7KK572 pKa = 9.63MSDD575 pKa = 3.48LSSIDD580 pKa = 3.45AQAAEE585 pKa = 4.43YY586 pKa = 9.64PSGYY590 pKa = 10.61EE591 pKa = 3.68FFASNSSPVYY601 pKa = 10.27GRR603 pKa = 11.84GSTEE607 pKa = 3.58VRR609 pKa = 11.84PKK611 pKa = 10.88AFTGVWVIRR620 pKa = 11.84ASGGFVAANTLWSVINGDD638 pKa = 3.25ATKK641 pKa = 9.8PANNTSVNGGEE652 pKa = 4.02IQSEE656 pKa = 4.37YY657 pKa = 10.76RR658 pKa = 11.84IGTQVEE664 pKa = 4.97GKK666 pKa = 8.65TSLRR670 pKa = 11.84FVGTIGQAYY679 pKa = 8.94AARR682 pKa = 11.84LNVSNNSNTNAFIYY696 pKa = 10.83DD697 pKa = 3.33FDD699 pKa = 3.98EE700 pKa = 4.44YY701 pKa = 11.64GRR703 pKa = 11.84VNFGKK708 pKa = 9.56PGGVIKK714 pKa = 10.98SGDD717 pKa = 3.3ITLDD721 pKa = 3.19MASNGDD727 pKa = 3.81FYY729 pKa = 11.68VTTTRR734 pKa = 11.84LRR736 pKa = 11.84VEE738 pKa = 4.53HH739 pKa = 6.51PTSGGQTMGISTQGGANRR757 pKa = 11.84GADD760 pKa = 3.37QGWFEE765 pKa = 4.38HH766 pKa = 6.49WGNSYY771 pKa = 11.26GGGSDD776 pKa = 3.09KK777 pKa = 11.24SRR779 pKa = 11.84ATVVEE784 pKa = 4.08LKK786 pKa = 10.52IGTGYY791 pKa = 11.22AWYY794 pKa = 9.32SQKK797 pKa = 10.58LASGVYY803 pKa = 6.28EE804 pKa = 4.06TRR806 pKa = 11.84FASAIYY812 pKa = 8.52ATAFTQNSDD821 pKa = 3.04EE822 pKa = 4.51RR823 pKa = 11.84IKK825 pKa = 11.2EE826 pKa = 4.05NIEE829 pKa = 4.38RR830 pKa = 11.84ISDD833 pKa = 3.77PLEE836 pKa = 3.81KK837 pKa = 9.72MKK839 pKa = 10.56QIKK842 pKa = 9.23GVSWNFKK849 pKa = 8.91TVGNAKK855 pKa = 10.08GYY857 pKa = 10.78GFIAQDD863 pKa = 3.51VEE865 pKa = 4.52RR866 pKa = 11.84VLPEE870 pKa = 3.84AVSVGGGEE878 pKa = 5.14LEE880 pKa = 4.25LTDD883 pKa = 3.67GTKK886 pKa = 10.39VSDD889 pKa = 3.75VKK891 pKa = 11.11SVNTSGVAAALHH903 pKa = 6.37HH904 pKa = 5.75EE905 pKa = 5.0AILALMDD912 pKa = 3.57KK913 pKa = 11.04VEE915 pKa = 4.11ALEE918 pKa = 4.07ARR920 pKa = 11.84IAEE923 pKa = 4.42LTSQQ927 pKa = 3.58

Molecular weight: 95.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6VUA2|H6VUA2_9CAUD Uncharacterized protein OS=Escherichia phage phiKT OX=1141519 GN=phiKT_0007 PE=4 SV=1
MM1 pKa = 7.58GEE3 pKa = 4.71CIEE6 pKa = 4.25HH7 pKa = 5.58TQVGDD12 pKa = 3.39LFGYY16 pKa = 10.89ANTRR20 pKa = 11.84RR21 pKa = 11.84GGRR24 pKa = 11.84PMKK27 pKa = 9.1MHH29 pKa = 6.86RR30 pKa = 11.84AVFLDD35 pKa = 2.89VHH37 pKa = 6.67GYY39 pKa = 8.6LPEE42 pKa = 4.32VVRR45 pKa = 11.84HH46 pKa = 5.15TCDD49 pKa = 2.85NPRR52 pKa = 11.84CINPEE57 pKa = 3.79HH58 pKa = 6.85LLAGTQKK65 pKa = 11.42DD66 pKa = 3.56NMRR69 pKa = 11.84DD70 pKa = 3.36CVEE73 pKa = 4.23RR74 pKa = 11.84GRR76 pKa = 11.84RR77 pKa = 11.84TPPPRR82 pKa = 11.84HH83 pKa = 5.89NKK85 pKa = 9.84LSQEE89 pKa = 3.9QVLEE93 pKa = 4.07IRR95 pKa = 11.84RR96 pKa = 11.84GTTPAKK102 pKa = 10.48DD103 pKa = 2.98LAEE106 pKa = 4.14RR107 pKa = 11.84FGVSKK112 pKa = 9.47VTIYY116 pKa = 10.86NVRR119 pKa = 11.84KK120 pKa = 9.79LKK122 pKa = 10.36RR123 pKa = 11.84GYY125 pKa = 9.71YY126 pKa = 9.85HH127 pKa = 7.36GG128 pKa = 4.4

Molecular weight: 14.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

11231

36

1400

362.3

39.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.275 ± 0.852

0.837 ± 0.189

6.135 ± 0.25

5.743 ± 0.4

3.428 ± 0.193

7.648 ± 0.331

1.834 ± 0.245

4.888 ± 0.222

5.529 ± 0.408

7.782 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.992 ± 0.323

4.425 ± 0.288

3.998 ± 0.314

4.461 ± 0.464

5.218 ± 0.273

6.34 ± 0.366

6.58 ± 0.423

6.963 ± 0.354

1.416 ± 0.106

3.508 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski