Paracoccus alcaliphilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4561 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8LWT4|A0A1H8LWT4_9RHOB 6-carboxy-5 6 7 8-tetrahydropterin synthase OS=Paracoccus alcaliphilus OX=34002 GN=SAMN04489859_103429 PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.21KK3 pKa = 10.04ISLPLFNALAGLVLVILLMAGTAQAQGLTMNITDD37 pKa = 3.99TDD39 pKa = 4.16YY40 pKa = 11.71DD41 pKa = 4.64PIPAGGEE48 pKa = 3.31INYY51 pKa = 9.83AVRR54 pKa = 11.84IQNGGNIRR62 pKa = 11.84TDD64 pKa = 3.05ARR66 pKa = 11.84DD67 pKa = 3.19ITLTIPANTTYY78 pKa = 10.43TGISGGLLNCEE89 pKa = 4.18PAPAITGPADD99 pKa = 3.27ITCQIPSLAPRR110 pKa = 11.84EE111 pKa = 4.11EE112 pKa = 4.13VQATVLVVPSLAGTIGFSGQVDD134 pKa = 4.02GQDD137 pKa = 3.08ILTQNTTVTVGADD150 pKa = 3.4LEE152 pKa = 4.7LGFVAPASIQAGEE165 pKa = 4.15FLSFQAVITNHH176 pKa = 5.66GPYY179 pKa = 10.18DD180 pKa = 3.88GGSTRR185 pKa = 11.84FTMLLPAALSHH196 pKa = 7.47DD197 pKa = 3.07ITMPAGCSIAGNTVTCDD214 pKa = 3.15VTGPIPADD222 pKa = 3.41GVITLDD228 pKa = 4.08FVTQVLTGDD237 pKa = 3.22II238 pKa = 4.1

Molecular weight: 24.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8M2X1|A0A1H8M2X1_9RHOB Hint domain-containing protein OS=Paracoccus alcaliphilus OX=34002 GN=SAMN04489859_103562 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.21GGQRR29 pKa = 11.84VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.69GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4561

0

4561

1370639

25

5731

300.5

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.678 ± 0.054

0.844 ± 0.013

6.137 ± 0.034

5.533 ± 0.033

3.494 ± 0.021

8.889 ± 0.046

2.135 ± 0.02

5.272 ± 0.025

2.488 ± 0.03

10.242 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.016

2.445 ± 0.021

5.409 ± 0.033

3.476 ± 0.022

7.646 ± 0.039

5.037 ± 0.025

5.187 ± 0.024

6.843 ± 0.03

1.471 ± 0.016

2.002 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski