Rattus norvegicus polyomavirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MVR9|A0A1J0MVR9_9POLY Large T antigen OS=Rattus norvegicus polyomavirus 2 OX=1919247 PE=4 SV=1
MM1 pKa = 7.65SRR3 pKa = 11.84VTCQRR8 pKa = 11.84KK9 pKa = 9.01RR10 pKa = 11.84LGPGRR15 pKa = 11.84APVQKK20 pKa = 10.32LPRR23 pKa = 11.84VIKK26 pKa = 10.36KK27 pKa = 10.66GGVEE31 pKa = 4.1VLATVPPGPEE41 pKa = 3.62SEE43 pKa = 4.67YY44 pKa = 10.71KK45 pKa = 10.79LEE47 pKa = 4.43MFLKK51 pKa = 10.24PCFGNDD57 pKa = 2.88SGQAGPHH64 pKa = 5.21YY65 pKa = 9.15WNHH68 pKa = 5.94SSPFTGEE75 pKa = 3.72ALTDD79 pKa = 3.47GDD81 pKa = 4.14YY82 pKa = 10.94HH83 pKa = 7.49VCYY86 pKa = 10.93SMGQIQLPEE95 pKa = 4.41ISDD98 pKa = 3.68QCSEE102 pKa = 4.09EE103 pKa = 4.37YY104 pKa = 9.77MVVWEE109 pKa = 4.7CYY111 pKa = 9.8RR112 pKa = 11.84MEE114 pKa = 4.28TEE116 pKa = 4.04VLYY119 pKa = 10.17TPKK122 pKa = 9.06ITAAGLSGSNFLAVQGTQMYY142 pKa = 8.12FWAVGGEE149 pKa = 4.19PLDD152 pKa = 3.74VMYY155 pKa = 9.59MLPKK159 pKa = 10.55EE160 pKa = 4.15KK161 pKa = 10.12MNPQGNLRR169 pKa = 11.84APSTTHH175 pKa = 6.35SVYY178 pKa = 10.72DD179 pKa = 3.72PNIIRR184 pKa = 11.84EE185 pKa = 4.2KK186 pKa = 10.53LSSEE190 pKa = 4.68LYY192 pKa = 9.43PVEE195 pKa = 4.22YY196 pKa = 7.27WTPDD200 pKa = 3.11PSRR203 pKa = 11.84NEE205 pKa = 3.43NTRR208 pKa = 11.84YY209 pKa = 9.31FGRR212 pKa = 11.84IVGGAATPPVVTYY225 pKa = 10.46SNSSTIPLLDD235 pKa = 3.7EE236 pKa = 4.58NGVGVLCNQQRR247 pKa = 11.84CYY249 pKa = 8.68VTCADD254 pKa = 3.16ITGFLHH260 pKa = 6.62NRR262 pKa = 11.84IEE264 pKa = 4.27TAHH267 pKa = 5.89GRR269 pKa = 11.84FFRR272 pKa = 11.84LHH274 pKa = 4.9FRR276 pKa = 11.84QRR278 pKa = 11.84RR279 pKa = 11.84VKK281 pKa = 10.65NPYY284 pKa = 9.26TMSLLYY290 pKa = 10.29KK291 pKa = 10.0QVLIQRR297 pKa = 11.84PPQVAAQLGVTEE309 pKa = 4.15VTIEE313 pKa = 4.03EE314 pKa = 4.49GQAPAPVMAQVGSCEE329 pKa = 4.44PMITQQASSMLNVQGG344 pKa = 4.06

Molecular weight: 38.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0MVR8|A0A1J0MVR8_9POLY Major capsid protein VP1 OS=Rattus norvegicus polyomavirus 2 OX=1919247 PE=3 SV=1
MM1 pKa = 7.41EE2 pKa = 6.0PEE4 pKa = 3.89NGICGGSNLTEE15 pKa = 4.01LGKK18 pKa = 10.29RR19 pKa = 11.84SKK21 pKa = 11.0SEE23 pKa = 3.64EE24 pKa = 3.93TGKK27 pKa = 10.52KK28 pKa = 9.04EE29 pKa = 4.35RR30 pKa = 11.84SGEE33 pKa = 3.98NNLIEE38 pKa = 4.38IVSKK42 pKa = 10.83VSKK45 pKa = 9.14TLPISPRR52 pKa = 11.84SRR54 pKa = 11.84GPVPKK59 pKa = 10.41GLLKK63 pKa = 10.94GPLKK67 pKa = 10.5ALRR70 pKa = 11.84VRR72 pKa = 11.84KK73 pKa = 7.8TFTVMRR79 pKa = 11.84NSLMSHH85 pKa = 6.43LRR87 pKa = 11.84RR88 pKa = 11.84NLLAIPKK95 pKa = 8.81QLPPKK100 pKa = 8.51RR101 pKa = 11.84IKK103 pKa = 10.3MIFLTFQNICWNLL116 pKa = 3.13

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2004

116

701

334.0

37.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.337 ± 1.507

2.495 ± 0.688

4.291 ± 0.775

6.836 ± 0.697

3.493 ± 0.836

7.036 ± 1.194

2.495 ± 0.362

4.691 ± 0.427

6.637 ± 1.437

9.182 ± 0.55

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.422

4.741 ± 0.361

5.639 ± 0.672

4.84 ± 0.652

5.988 ± 0.695

5.988 ± 0.684

5.589 ± 0.413

6.537 ± 0.645

1.397 ± 0.233

3.244 ± 0.543
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski