Sinorhizobium fredii GR64

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium; Sinorhizobium fredii group; Sinorhizobium fredii

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7XYZ7|F7XYZ7_RHIFR Hypothetical conserved protein OS=Sinorhizobium fredii GR64 OX=882101 GN=SFGR64A_00122 PE=4 SV=1
MM1 pKa = 7.38SHH3 pKa = 7.5DD4 pKa = 3.85LSLAQSHH11 pKa = 6.68AFQLSRR17 pKa = 11.84DD18 pKa = 3.49LMVPVTLFEE27 pKa = 4.25VDD29 pKa = 3.03GEE31 pKa = 4.56YY32 pKa = 11.23GVLPSDD38 pKa = 5.39EE39 pKa = 4.67IDD41 pKa = 3.87ADD43 pKa = 4.03DD44 pKa = 4.78DD45 pKa = 4.31LEE47 pKa = 4.45IVHH50 pKa = 7.13EE51 pKa = 4.18YY52 pKa = 9.4TPWHH56 pKa = 6.08

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7XYS5|F7XYS5_RHIFR Oxidoreductase protein OS=Sinorhizobium fredii GR64 OX=882101 GN=SFGR64A_00050 PE=3 SV=1
MM1 pKa = 7.3PVEE4 pKa = 4.04PVGYY8 pKa = 8.91MSKK11 pKa = 9.59IDD13 pKa = 3.48RR14 pKa = 11.84GGEE17 pKa = 4.06QKK19 pKa = 9.53STTDD23 pKa = 3.47LADD26 pKa = 3.49TRR28 pKa = 11.84SEE30 pKa = 4.62GPFGRR35 pKa = 11.84VRR37 pKa = 11.84SQIDD41 pKa = 3.25EE42 pKa = 4.38AFPTSRR48 pKa = 11.84RR49 pKa = 11.84HH50 pKa = 5.29PRR52 pKa = 11.84VNTPHH57 pKa = 6.58RR58 pKa = 11.84CVRR61 pKa = 11.84SSSLQVVGGVAYY73 pKa = 9.42VVRR76 pKa = 11.84RR77 pKa = 11.84MSCRR81 pKa = 11.84SQPADD86 pKa = 2.97MVRR89 pKa = 11.84PHH91 pKa = 6.46KK92 pKa = 10.42RR93 pKa = 11.84PRR95 pKa = 11.84SAGALLALVPSAEE108 pKa = 4.3KK109 pKa = 9.91IHH111 pKa = 6.91RR112 pKa = 11.84DD113 pKa = 3.62GVSGTPSSGDD123 pKa = 3.37VQQ125 pKa = 3.55

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

166

0

166

54098

45

1699

325.9

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.396 ± 0.238

0.828 ± 0.055

5.627 ± 0.14

6.117 ± 0.17

3.667 ± 0.086

8.289 ± 0.185

2.022 ± 0.069

5.721 ± 0.12

3.754 ± 0.137

9.716 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.074

2.952 ± 0.092

4.436 ± 0.155

3.355 ± 0.124

6.98 ± 0.164

6.533 ± 0.145

5.2 ± 0.101

7.632 ± 0.156

1.244 ± 0.078

2.124 ± 0.086
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski