Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Parastagonospora nodorum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Phaeosphaeriaceae; Parastagonospora; Parastagonospora nodorum

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0V2I3|Q0V2I3_PHANO Uncharacterized protein OS=Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) OX=321614 GN=SNOG_01781 PE=4 SV=2
MM1 pKa = 7.53AAATHH6 pKa = 6.65AVRR9 pKa = 11.84KK10 pKa = 9.83LDD12 pKa = 3.79AATDD16 pKa = 4.47DD17 pKa = 4.61IMDD20 pKa = 4.21LHH22 pKa = 7.55SDD24 pKa = 3.44SGLDD28 pKa = 4.33FDD30 pKa = 6.49DD31 pKa = 5.76GDD33 pKa = 4.23IDD35 pKa = 6.16LDD37 pKa = 4.48LDD39 pKa = 4.03PAPPAQADD47 pKa = 3.95DD48 pKa = 4.85DD49 pKa = 5.35DD50 pKa = 6.09ISLKK54 pKa = 10.88DD55 pKa = 3.54AGADD59 pKa = 3.33ADD61 pKa = 5.45LEE63 pKa = 4.57TQTVSPDD70 pKa = 2.91QDD72 pKa = 3.79MVDD75 pKa = 4.07HH76 pKa = 6.55EE77 pKa = 4.8VDD79 pKa = 4.09DD80 pKa = 5.03EE81 pKa = 4.85GDD83 pKa = 3.32IQLGDD88 pKa = 4.71GIHH91 pKa = 7.41DD92 pKa = 3.67DD93 pKa = 4.51HH94 pKa = 9.39VPIDD98 pKa = 4.0YY99 pKa = 7.7TTPAQEE105 pKa = 4.1TAPAHH110 pKa = 6.62PDD112 pKa = 3.48DD113 pKa = 5.88DD114 pKa = 6.36LIDD117 pKa = 3.99YY118 pKa = 11.09SDD120 pKa = 5.63DD121 pKa = 3.26EE122 pKa = 5.72DD123 pKa = 4.1IYY125 pKa = 10.34PTPTYY130 pKa = 10.71NQGTPAQSVGQTQDD144 pKa = 3.19VQNDD148 pKa = 3.29EE149 pKa = 4.5SASEE153 pKa = 3.96IHH155 pKa = 6.64ALPAEE160 pKa = 4.11AQIPGSTNEE169 pKa = 4.83DD170 pKa = 3.59EE171 pKa = 4.62IANHH175 pKa = 6.51GDD177 pKa = 3.56YY178 pKa = 11.39ADD180 pKa = 4.57GEE182 pKa = 5.13DD183 pKa = 3.67NFEE186 pKa = 4.12PSAEE190 pKa = 3.98NEE192 pKa = 4.22EE193 pKa = 4.11QHH195 pKa = 7.08DD196 pKa = 4.27EE197 pKa = 4.39NNFEE201 pKa = 4.3PSAEE205 pKa = 3.99NQEE208 pKa = 4.14QHH210 pKa = 7.28DD211 pKa = 4.08EE212 pKa = 4.52TEE214 pKa = 4.64FRR216 pKa = 11.84AQDD219 pKa = 3.34EE220 pKa = 4.74AEE222 pKa = 4.12YY223 pKa = 8.87DD224 pKa = 3.52TDD226 pKa = 4.17GDD228 pKa = 3.93TGGVPLQDD236 pKa = 3.45QDD238 pKa = 3.75SQADD242 pKa = 3.46VSEE245 pKa = 4.43YY246 pKa = 9.98QQGDD250 pKa = 3.67HH251 pKa = 6.93VEE253 pKa = 4.13QQSIEE258 pKa = 4.12SRR260 pKa = 11.84PITINYY266 pKa = 7.46EE267 pKa = 4.38GNEE270 pKa = 3.56LWLFKK275 pKa = 10.72QHH277 pKa = 7.24DD278 pKa = 4.08VDD280 pKa = 5.85EE281 pKa = 5.31SGDD284 pKa = 3.51WLTEE288 pKa = 3.84DD289 pKa = 3.55TSIIHH294 pKa = 6.35SSLSEE299 pKa = 3.85MFQACRR305 pKa = 11.84ASLGEE310 pKa = 4.23NISNEE315 pKa = 4.22TEE317 pKa = 3.52LGLRR321 pKa = 11.84FDD323 pKa = 4.7HH324 pKa = 6.14FHH326 pKa = 7.02NMEE329 pKa = 3.94IFEE332 pKa = 5.24DD333 pKa = 3.86STACVAVSLGRR344 pKa = 11.84MIDD347 pKa = 3.69LYY349 pKa = 10.25YY350 pKa = 10.4TLHH353 pKa = 6.08AQDD356 pKa = 4.94GEE358 pKa = 4.53SEE360 pKa = 4.3PEE362 pKa = 3.94SFYY365 pKa = 10.81MCLLSRR371 pKa = 11.84PRR373 pKa = 11.84FATLLSDD380 pKa = 3.7IAKK383 pKa = 9.87HH384 pKa = 6.25ADD386 pKa = 3.14QGHH389 pKa = 6.8GYY391 pKa = 10.13SGLNSAIAAGEE402 pKa = 4.13THH404 pKa = 6.73FVDD407 pKa = 3.4AHH409 pKa = 6.33SGQSTEE415 pKa = 4.43HH416 pKa = 7.01EE417 pKa = 4.39GTEE420 pKa = 3.84WDD422 pKa = 3.49QDD424 pKa = 3.59AEE426 pKa = 4.29GQEE429 pKa = 4.3EE430 pKa = 4.57YY431 pKa = 10.85GQKK434 pKa = 10.55EE435 pKa = 3.93EE436 pKa = 4.44TEE438 pKa = 4.07QEE440 pKa = 4.05EE441 pKa = 4.66NVAQEE446 pKa = 4.01NAQEE450 pKa = 4.12GTEE453 pKa = 4.24IVDD456 pKa = 3.67EE457 pKa = 4.73AADD460 pKa = 3.79EE461 pKa = 4.29AATGIDD467 pKa = 3.9LGEE470 pKa = 5.28NDD472 pKa = 5.18DD473 pKa = 4.31QDD475 pKa = 3.87NDD477 pKa = 3.49EE478 pKa = 4.35PRR480 pKa = 11.84VNGEE484 pKa = 3.85EE485 pKa = 4.12HH486 pKa = 6.52SSKK489 pKa = 11.32AEE491 pKa = 4.09TKK493 pKa = 10.08SEE495 pKa = 4.15HH496 pKa = 6.65ASTPAAVAHH505 pKa = 6.42GSSPAAQEE513 pKa = 4.21SPDD516 pKa = 3.45RR517 pKa = 11.84SAEE520 pKa = 4.19TTQHH524 pKa = 6.94DD525 pKa = 3.84VTANQTAEE533 pKa = 3.85QLANDD538 pKa = 4.05TVDD541 pKa = 3.51YY542 pKa = 11.37SDD544 pKa = 6.17DD545 pKa = 4.03EE546 pKa = 5.72DD547 pKa = 6.49DD548 pKa = 4.64EE549 pKa = 4.93PQTQAPTNASPSSTTVQGDD568 pKa = 3.46NPATIEE574 pKa = 3.98ASAPTAEE581 pKa = 4.2STSFEE586 pKa = 4.23KK587 pKa = 10.42EE588 pKa = 3.94YY589 pKa = 9.64QTGYY593 pKa = 11.43DD594 pKa = 3.45DD595 pKa = 4.87AFPYY599 pKa = 9.75AANGAYY605 pKa = 9.67EE606 pKa = 5.08DD607 pKa = 4.12YY608 pKa = 11.39AHH610 pKa = 7.07NEE612 pKa = 3.84EE613 pKa = 5.45QFDD616 pKa = 4.04HH617 pKa = 6.81EE618 pKa = 5.07NNADD622 pKa = 3.49MYY624 pKa = 10.96QEE626 pKa = 3.93YY627 pKa = 10.59DD628 pKa = 3.12GTYY631 pKa = 10.94GEE633 pKa = 5.48GEE635 pKa = 4.15ASDD638 pKa = 5.04LQTNDD643 pKa = 2.36QDD645 pKa = 4.3AYY647 pKa = 9.95PAEE650 pKa = 4.88ADD652 pKa = 4.31LDD654 pKa = 3.95GLTNQDD660 pKa = 3.31YY661 pKa = 9.87TGYY664 pKa = 10.67DD665 pKa = 3.65YY666 pKa = 11.52QDD668 pKa = 4.73LEE670 pKa = 4.18QQLQNEE676 pKa = 5.26FISGGEE682 pKa = 3.83VDD684 pKa = 3.58GTGAGEE690 pKa = 4.38SANGAHH696 pKa = 6.52EE697 pKa = 5.1LVDD700 pKa = 4.38GDD702 pKa = 5.62DD703 pKa = 5.43FLDD706 pKa = 4.71LEE708 pKa = 4.89NATEE712 pKa = 3.89WGTDD716 pKa = 2.85QGQAVEE722 pKa = 4.14VSGDD726 pKa = 3.69NIFLDD731 pKa = 4.45DD732 pKa = 4.56EE733 pKa = 4.7VTAHH737 pKa = 6.23YY738 pKa = 10.23EE739 pKa = 3.99EE740 pKa = 5.16EE741 pKa = 4.07EE742 pKa = 4.62GGVVEE747 pKa = 4.74QPAVAASSAADD758 pKa = 3.44PVAASSTDD766 pKa = 3.69LQDD769 pKa = 5.59SSPQGQKK776 pKa = 10.13RR777 pKa = 11.84SRR779 pKa = 11.84DD780 pKa = 3.52EE781 pKa = 4.94ASDD784 pKa = 3.94SVGDD788 pKa = 3.98ALDD791 pKa = 3.72PSGTHH796 pKa = 6.86HH797 pKa = 5.98IHH799 pKa = 7.49GINLCWQDD807 pKa = 3.2ADD809 pKa = 4.98FVFPDD814 pKa = 3.73TKK816 pKa = 10.46RR817 pKa = 11.84PRR819 pKa = 11.84VV820 pKa = 3.4

Molecular weight: 89.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0UFA5|Q0UFA5_PHANO Uncharacterized protein OS=Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) OX=321614 GN=SNOG_09559 PE=4 SV=1
MM1 pKa = 7.37QIFGNSSRR9 pKa = 11.84GRR11 pKa = 11.84RR12 pKa = 11.84LRR14 pKa = 11.84WKK16 pKa = 10.5LRR18 pKa = 11.84LLTQSTTSRR27 pKa = 11.84ARR29 pKa = 11.84SRR31 pKa = 11.84TRR33 pKa = 11.84RR34 pKa = 11.84AFPRR38 pKa = 11.84TSSVSFSPASSSRR51 pKa = 11.84MAALFRR57 pKa = 11.84TTTSRR62 pKa = 11.84RR63 pKa = 11.84SPPSII68 pKa = 3.88

Molecular weight: 7.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15997

1

15998

6077303

21

7045

379.9

42.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.89 ± 0.019

1.278 ± 0.007

5.729 ± 0.015

6.21 ± 0.019

3.706 ± 0.014

6.747 ± 0.02

2.468 ± 0.01

4.868 ± 0.015

5.131 ± 0.02

8.62 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.008

3.717 ± 0.013

5.93 ± 0.02

4.068 ± 0.015

5.97 ± 0.017

7.922 ± 0.024

6.07 ± 0.017

6.084 ± 0.014

1.495 ± 0.008

2.765 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski