Mycoplasma anatis 1340

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis; Mycoplasmopsis anatis

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9QD64|F9QD64_9MOLU Adenine phosphoribosyltransferase OS=Mycoplasma anatis 1340 OX=1034808 GN=apt PE=3 SV=1
MM1 pKa = 7.66KK2 pKa = 10.58NNNNDD7 pKa = 2.73IYY9 pKa = 10.64ICGGLSIYY17 pKa = 10.45LQTINIADD25 pKa = 3.99EE26 pKa = 4.81VILSVISSKK35 pKa = 11.43YY36 pKa = 9.95EE37 pKa = 3.59GDD39 pKa = 3.04IKK41 pKa = 10.88IFF43 pKa = 3.65

Molecular weight: 4.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9QD38|F9QD38_9MOLU 50S ribosomal protein L14 OS=Mycoplasma anatis 1340 OX=1034808 GN=rplN PE=3 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 3.93KK9 pKa = 10.2AKK11 pKa = 10.03RR12 pKa = 11.84HH13 pKa = 5.81PKK15 pKa = 10.03FSTRR19 pKa = 11.84AYY21 pKa = 8.48TRR23 pKa = 11.84CEE25 pKa = 3.78LCGRR29 pKa = 11.84PHH31 pKa = 6.83SVLRR35 pKa = 11.84KK36 pKa = 9.37YY37 pKa = 10.29KK38 pKa = 9.48ICRR41 pKa = 11.84ICFRR45 pKa = 11.84GLANEE50 pKa = 4.21GKK52 pKa = 10.0IPGMKK57 pKa = 9.27KK58 pKa = 10.56ASWW61 pKa = 3.19

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

803

0

803

275923

30

4077

343.6

39.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.493 ± 0.088

0.577 ± 0.025

5.369 ± 0.062

7.062 ± 0.101

5.406 ± 0.09

4.363 ± 0.088

1.321 ± 0.03

10.125 ± 0.115

10.119 ± 0.086

9.556 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.04

8.542 ± 0.147

2.441 ± 0.05

3.001 ± 0.055

2.961 ± 0.052

7.014 ± 0.069

5.097 ± 0.061

5.569 ± 0.083

0.893 ± 0.028

4.33 ± 0.075
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski