Desulfomonile tiedjei (strain ATCC 49306 / DSM 6799 / DCB-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Syntrophobacterales; Syntrophaceae; Desulfomonile; Desulfomonile tiedjei

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5412 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4CEW6|I4CEW6_DESTA Uncharacterized protein OS=Desulfomonile tiedjei (strain ATCC 49306 / DSM 6799 / DCB-1) OX=706587 GN=Desti_5525 PE=4 SV=1
MM1 pKa = 7.17VFANMRR7 pKa = 11.84KK8 pKa = 9.11LCRR11 pKa = 11.84EE12 pKa = 3.98LVLEE16 pKa = 4.15PLEE19 pKa = 4.45DD20 pKa = 3.55RR21 pKa = 11.84VVLDD25 pKa = 4.45AYY27 pKa = 10.98VDD29 pKa = 3.58PVYY32 pKa = 10.79QSDD35 pKa = 3.66ITVVDD40 pKa = 3.99ALAAVNHH47 pKa = 5.98EE48 pKa = 4.6PIAYY52 pKa = 8.25AQSITLVEE60 pKa = 4.23NQVSQITLTGDD71 pKa = 3.6DD72 pKa = 4.26GDD74 pKa = 4.48PDD76 pKa = 4.09VVQSLTFFLDD86 pKa = 3.51SVPANGALYY95 pKa = 10.6LSQADD100 pKa = 4.0AQNGTNPLGVGASLTTGVLWYY121 pKa = 10.46KK122 pKa = 10.62SALNNDD128 pKa = 3.31MDD130 pKa = 4.25TSFMFHH136 pKa = 6.21VKK138 pKa = 10.52DD139 pKa = 5.17DD140 pKa = 3.85GGTANGGDD148 pKa = 3.91DD149 pKa = 3.69TSASALVTVTVAPDD163 pKa = 3.61NQSPDD168 pKa = 3.12AGNFSVVVNEE178 pKa = 3.83DD179 pKa = 3.23TAIKK183 pKa = 8.21VTGWNFTDD191 pKa = 4.03AEE193 pKa = 4.64GNQGQSIRR201 pKa = 11.84ITDD204 pKa = 4.01LPDD207 pKa = 3.89HH208 pKa = 7.05GILFIDD214 pKa = 3.97ANDD217 pKa = 3.82NNVVDD222 pKa = 4.41PGEE225 pKa = 4.93AIGSDD230 pKa = 3.41QLNGRR235 pKa = 11.84VTFTFDD241 pKa = 4.48DD242 pKa = 5.27GYY244 pKa = 11.22LNNYY248 pKa = 8.63INAFPIMEE256 pKa = 4.36EE257 pKa = 4.14YY258 pKa = 10.63GVEE261 pKa = 4.2GVSLVVTGDD270 pKa = 2.69IGQNAEE276 pKa = 3.76AMTWSQLQEE285 pKa = 3.67MQAAGWEE292 pKa = 4.32IGSHH296 pKa = 5.76SMTHH300 pKa = 6.31PMLTEE305 pKa = 3.97LTDD308 pKa = 3.49SQLVYY313 pKa = 10.59EE314 pKa = 4.67LSEE317 pKa = 4.43SKK319 pKa = 10.9NLLVQHH325 pKa = 6.78GLTGTSFAYY334 pKa = 9.52PYY336 pKa = 11.07GDD338 pKa = 3.4FDD340 pKa = 4.22PRR342 pKa = 11.84VVEE345 pKa = 4.06YY346 pKa = 11.61VMDD349 pKa = 3.91YY350 pKa = 11.6YY351 pKa = 10.83EE352 pKa = 4.51GCRR355 pKa = 11.84EE356 pKa = 3.56AWGNNGINEE365 pKa = 4.54TPGNPYY371 pKa = 10.32AIYY374 pKa = 10.43NRR376 pKa = 11.84SVDD379 pKa = 3.51NTTTPQEE386 pKa = 4.15VIQWINDD393 pKa = 3.33AVANNYY399 pKa = 8.65WLVISFHH406 pKa = 6.9HH407 pKa = 6.52IVTGTPGPYY416 pKa = 9.55QYY418 pKa = 11.31NVNNFEE424 pKa = 4.58TIVSYY429 pKa = 10.8VASHH433 pKa = 6.99NIATPTISEE442 pKa = 4.27VLNNDD447 pKa = 4.24LAPISWADD455 pKa = 3.14ATTKK459 pKa = 10.5LKK461 pKa = 11.13YY462 pKa = 9.84IGSQDD467 pKa = 3.66YY468 pKa = 10.75YY469 pKa = 11.24GQDD472 pKa = 2.83SFQYY476 pKa = 10.88VVIDD480 pKa = 3.65SAGSEE485 pKa = 3.9GTMPVDD491 pKa = 3.97AGTTTVTVNPVNDD504 pKa = 4.2DD505 pKa = 4.51PINDD509 pKa = 3.66EE510 pKa = 5.13PIANPQSITLTEE522 pKa = 4.08NQVTQITLTGDD533 pKa = 3.69DD534 pKa = 4.44GDD536 pKa = 4.61PDD538 pKa = 3.72VTQTLTFFLDD548 pKa = 3.45SLPANGALYY557 pKa = 10.63LSQADD562 pKa = 3.93AQSGTNPLGVGASLSTGVLWYY583 pKa = 10.59KK584 pKa = 10.77SGLNADD590 pKa = 3.99ADD592 pKa = 4.12TSFMFHH598 pKa = 6.2VKK600 pKa = 10.73DD601 pKa = 4.18NGGTANGGDD610 pKa = 3.91DD611 pKa = 3.69TSASALVTVTVTPDD625 pKa = 3.31NQSPDD630 pKa = 3.25AGNFSTVVNEE640 pKa = 3.89DD641 pKa = 3.26TAVNVTGWNFTDD653 pKa = 4.38AEE655 pKa = 4.54GNQAQSIRR663 pKa = 11.84ITDD666 pKa = 4.02LPDD669 pKa = 3.76HH670 pKa = 6.26GTLFIDD676 pKa = 4.22ANDD679 pKa = 3.86NNVVDD684 pKa = 4.33PGEE687 pKa = 4.81AISLQPNEE695 pKa = 3.92GRR697 pKa = 11.84VTFTFDD703 pKa = 4.28DD704 pKa = 5.41GYY706 pKa = 11.63LDD708 pKa = 3.72TYY710 pKa = 10.68TNAFRR715 pKa = 11.84IMDD718 pKa = 4.0TYY720 pKa = 11.3DD721 pKa = 3.23VEE723 pKa = 4.82GVSLVVTGDD732 pKa = 3.55IGDD735 pKa = 3.6AWAMTWSQLQQMQAAGWEE753 pKa = 4.34IGSHH757 pKa = 5.76SMTHH761 pKa = 6.26PMLTGLTDD769 pKa = 3.38SQLVYY774 pKa = 10.59EE775 pKa = 4.67LSEE778 pKa = 4.48SKK780 pKa = 10.93NLLDD784 pKa = 4.44QYY786 pKa = 11.46GLTGTSFAYY795 pKa = 9.52PYY797 pKa = 11.07GDD799 pKa = 3.45FDD801 pKa = 3.98PRR803 pKa = 11.84VAEE806 pKa = 4.33FVSRR810 pKa = 11.84YY811 pKa = 9.99YY812 pKa = 10.66EE813 pKa = 4.03DD814 pKa = 5.22CRR816 pKa = 11.84EE817 pKa = 3.68AWGNNGINEE826 pKa = 4.3IPGNPYY832 pKa = 10.21AIYY835 pKa = 10.43NRR837 pKa = 11.84SVDD840 pKa = 3.51NTTTPQEE847 pKa = 4.15VIQWINDD854 pKa = 3.33AVANNYY860 pKa = 8.65WLVISFHH867 pKa = 6.84RR868 pKa = 11.84IVDD871 pKa = 4.18GTANPYY877 pKa = 9.9EE878 pKa = 4.12YY879 pKa = 10.75NVNNFEE885 pKa = 4.61TIVSYY890 pKa = 10.8VASHH894 pKa = 6.99NIATPTISEE903 pKa = 4.36VVNNDD908 pKa = 3.45LAPISWADD916 pKa = 3.14ATTKK920 pKa = 10.51LKK922 pKa = 11.13YY923 pKa = 10.28IGDD926 pKa = 3.86QEE928 pKa = 4.55YY929 pKa = 10.86SGSDD933 pKa = 2.83SFQYY937 pKa = 11.01VVIDD941 pKa = 3.66SAGSEE946 pKa = 4.11GKK948 pKa = 9.02MPADD952 pKa = 3.87AGTAIVTVIAVNDD965 pKa = 3.83APVNSIPGPQTVNGGTDD982 pKa = 3.67LLFSTANGNAISISDD997 pKa = 3.66ADD999 pKa = 3.88AGTEE1003 pKa = 3.91AVRR1006 pKa = 11.84VQLTATHH1013 pKa = 6.04GTLTLSGTSGLSFTAGDD1030 pKa = 3.95GTTDD1034 pKa = 2.76ATMTFTGSMTSINAALNGMHH1054 pKa = 6.35FTPTSNYY1061 pKa = 10.35SGDD1064 pKa = 3.4ATVQIVTNDD1073 pKa = 3.39LGNNPSGEE1081 pKa = 4.16MSDD1084 pKa = 3.48SDD1086 pKa = 4.01TVNITVVSTSGPPVAGDD1103 pKa = 3.2FAVSVNEE1110 pKa = 4.17DD1111 pKa = 2.98AVTTVSQWTFTDD1123 pKa = 3.78PNGDD1127 pKa = 3.44SAQSIRR1133 pKa = 11.84ITDD1136 pKa = 4.02LPDD1139 pKa = 3.78HH1140 pKa = 6.27GTLFLDD1146 pKa = 4.13ANTNNILDD1154 pKa = 3.95ANEE1157 pKa = 4.09AVSLNQVISWADD1169 pKa = 2.95AKK1171 pKa = 10.18TSPKK1175 pKa = 9.98VKK1177 pKa = 10.57YY1178 pKa = 10.44VGSQNYY1184 pKa = 9.53NGQDD1188 pKa = 2.99SLAYY1192 pKa = 10.19VVIDD1196 pKa = 3.36SSGEE1200 pKa = 3.77EE1201 pKa = 4.33GVAPDD1206 pKa = 3.96GDD1208 pKa = 4.16GTGSITVTAVNDD1220 pKa = 3.77APVNAVPVAQTMNANTSLIFSTANGNVISISDD1252 pKa = 3.51VDD1254 pKa = 3.88AGTDD1258 pKa = 3.43SVRR1261 pKa = 11.84VQLTATNGNLTLSGIGGLSFSAGDD1285 pKa = 3.73GTADD1289 pKa = 3.04ATMTFTGSVASINAALNGMSFTPTSNYY1316 pKa = 10.31SGAATVQIVTNDD1328 pKa = 3.45LGHH1331 pKa = 6.2NPSGEE1336 pKa = 4.1MSDD1339 pKa = 3.48SDD1341 pKa = 4.01TVNITVVSTSGPPVAGDD1358 pKa = 3.2FAVSVNEE1365 pKa = 4.82DD1366 pKa = 2.7IVATVSQWTFTDD1378 pKa = 3.78PNGDD1382 pKa = 3.44SAQSIRR1388 pKa = 11.84ITDD1391 pKa = 4.02LPDD1394 pKa = 3.78HH1395 pKa = 6.27GTLFLDD1401 pKa = 4.13ANTNNILDD1409 pKa = 3.95ANEE1412 pKa = 4.09AVSLNQVISWADD1424 pKa = 2.95AKK1426 pKa = 10.18TSPKK1430 pKa = 9.98VKK1432 pKa = 10.57YY1433 pKa = 10.44VGSQNYY1439 pKa = 9.45NGQDD1443 pKa = 2.99SLTYY1447 pKa = 10.62VVIDD1451 pKa = 3.38SSGEE1455 pKa = 3.89EE1456 pKa = 4.32GVAPAGDD1463 pKa = 3.91GTGSITVAAVNDD1475 pKa = 3.99APVNAIPAAQTMNANTSLIFSTANGNVISISDD1507 pKa = 3.51VDD1509 pKa = 4.25AGTDD1513 pKa = 3.7SISVQLTATNGNLTLAGTSGLSFTAGDD1540 pKa = 3.83GTADD1544 pKa = 3.09ATMTFTGSVASINAALNGMHH1564 pKa = 6.35FTPTSNYY1571 pKa = 9.76SGAASVQIVTNDD1583 pKa = 3.38LGHH1586 pKa = 6.2NPSGEE1591 pKa = 4.1MSDD1594 pKa = 3.48SDD1596 pKa = 4.01TVNITVVSAGGPPVADD1612 pKa = 4.71DD1613 pKa = 3.51FTLSVKK1619 pKa = 10.26EE1620 pKa = 4.04DD1621 pKa = 3.63TVVTVSKK1628 pKa = 8.47WTFTDD1633 pKa = 3.37PDD1635 pKa = 4.07GNSAQSIRR1643 pKa = 11.84ITDD1646 pKa = 4.02LPDD1649 pKa = 3.78HH1650 pKa = 6.27GTLFLDD1656 pKa = 4.12ANANNILDD1664 pKa = 3.76TSEE1667 pKa = 5.38AISLNQVISWTDD1679 pKa = 2.8AKK1681 pKa = 8.47TTPKK1685 pKa = 10.14VKK1687 pKa = 10.65YY1688 pKa = 10.29VGSQNYY1694 pKa = 9.53NGQDD1698 pKa = 2.99SLAYY1702 pKa = 10.19VVIDD1706 pKa = 3.38SSGQEE1711 pKa = 4.51GIPSAGDD1718 pKa = 3.3GTGTITVTAVNDD1730 pKa = 3.77APVNAVPGSQSINEE1744 pKa = 4.13DD1745 pKa = 3.43TVLTFSAANGNAISISDD1762 pKa = 3.54VDD1764 pKa = 4.13AGTEE1768 pKa = 3.97PVRR1771 pKa = 11.84VTLTATRR1778 pKa = 11.84GKK1780 pKa = 8.92LTLSGTTGLTFSTGDD1795 pKa = 3.37GTADD1799 pKa = 3.0TTMTFTGSIDD1809 pKa = 4.87DD1810 pKa = 4.47INAALNGMNFTPNANYY1826 pKa = 10.59AGAATVKK1833 pKa = 10.28IVTNDD1838 pKa = 3.25LGNNPSGAKK1847 pKa = 9.87SDD1849 pKa = 3.62TDD1851 pKa = 3.97TIAVTVNAIDD1861 pKa = 4.21DD1862 pKa = 4.18APVNSVPGTQTVSAGQTLVFNNVNGNRR1889 pKa = 11.84IRR1891 pKa = 11.84VSDD1894 pKa = 3.62VDD1896 pKa = 3.75AGANQIEE1903 pKa = 4.74VTLTAANGTLTLYY1916 pKa = 10.94SLTGLTFSAGDD1927 pKa = 3.69GTNDD1931 pKa = 2.7EE1932 pKa = 4.72SMTFRR1937 pKa = 11.84GTVSSINAAINRR1949 pKa = 11.84LRR1951 pKa = 11.84FIPTIAGSASLSITTNDD1968 pKa = 3.57LGASGSGGPLTDD1980 pKa = 3.6TDD1982 pKa = 4.53SIQINVTT1989 pKa = 3.25

Molecular weight: 208.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4CAM2|I4CAM2_DESTA Uncharacterized protein OS=Desulfomonile tiedjei (strain ATCC 49306 / DSM 6799 / DCB-1) OX=706587 GN=Desti_3971 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLSRR12 pKa = 11.84KK13 pKa = 7.47RR14 pKa = 11.84THH16 pKa = 6.28GFRR19 pKa = 11.84IRR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 9.83GGRR28 pKa = 11.84LVLQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.59GRR39 pKa = 11.84KK40 pKa = 8.55RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5412

0

5412

1757775

29

4853

324.8

36.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.357 ± 0.038

1.331 ± 0.015

5.386 ± 0.044

6.611 ± 0.041

4.324 ± 0.025

7.514 ± 0.049

2.055 ± 0.016

6.456 ± 0.026

5.134 ± 0.04

10.04 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.017

3.395 ± 0.036

4.727 ± 0.027

3.224 ± 0.019

6.029 ± 0.041

6.309 ± 0.026

5.173 ± 0.047

7.264 ± 0.03

1.226 ± 0.016

2.827 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski