Sphingopyxis sp. QXT-31

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis; unclassified Sphingopyxis

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8WW12|A0A1P8WW12_9SPHN FMN_red domain-containing protein OS=Sphingopyxis sp. QXT-31 OX=1357916 GN=BWQ93_06785 PE=4 SV=1
MM1 pKa = 7.26VLPSRR6 pKa = 11.84LQTVGEE12 pKa = 4.26SGNVFIDD19 pKa = 3.36TLVYY23 pKa = 10.39GVAFRR28 pKa = 11.84PGTTVIYY35 pKa = 10.32ALQGNPGDD43 pKa = 3.66TGLNGGAAWAANGAASAYY61 pKa = 9.93AAALASWSAVANIQFQAATVAYY83 pKa = 9.6DD84 pKa = 3.69GTGSRR89 pKa = 11.84AGYY92 pKa = 10.43DD93 pKa = 3.27FVGRR97 pKa = 11.84LSTTLPDD104 pKa = 3.72GVLGQHH110 pKa = 6.28TLPTTGDD117 pKa = 3.26MVGEE121 pKa = 4.35FNTTISYY128 pKa = 7.51FTAASNQPGGLSFLTFVHH146 pKa = 6.29EE147 pKa = 4.48LGHH150 pKa = 6.94GIGLLHH156 pKa = 6.58PHH158 pKa = 6.94ADD160 pKa = 3.72EE161 pKa = 5.56PGDD164 pKa = 4.22DD165 pKa = 4.39SFPGLNDD172 pKa = 3.33AFEE175 pKa = 4.45TGPFGLNQGIFTTMTYY191 pKa = 10.8NDD193 pKa = 4.11GYY195 pKa = 10.03EE196 pKa = 4.15DD197 pKa = 3.6VGRR200 pKa = 11.84SPSANYY206 pKa = 8.44GWQMGPGAFDD216 pKa = 3.28IAAVQYY222 pKa = 10.77LYY224 pKa = 11.14GANTTTGSGNTTYY237 pKa = 11.08TMPTLNQAGTGWVTIWDD254 pKa = 3.65VGGTDD259 pKa = 3.78TISAQGSQANAIIDD273 pKa = 3.77LRR275 pKa = 11.84AATLRR280 pKa = 11.84NEE282 pKa = 4.07EE283 pKa = 4.14GGGGFVSRR291 pKa = 11.84NGGVLGGFTIANGAVIEE308 pKa = 4.03NAIGGSGNDD317 pKa = 3.26RR318 pKa = 11.84IVGNSANNNLNGGDD332 pKa = 3.43GWDD335 pKa = 3.8TIVYY339 pKa = 9.67SHH341 pKa = 6.21MTAGVTVNLQAGTSTGDD358 pKa = 3.33GTDD361 pKa = 3.2TLVSIEE367 pKa = 4.06NAIGGNGDD375 pKa = 5.28DD376 pKa = 4.81ILTAINGRR384 pKa = 11.84DD385 pKa = 3.41IANGTQLFVKK395 pKa = 9.15TATAIISSRR404 pKa = 11.84ATAYY408 pKa = 11.1SLDD411 pKa = 3.32GLFRR415 pKa = 11.84GSGSNYY421 pKa = 9.45SVSIQAEE428 pKa = 4.35GSGGADD434 pKa = 2.8HH435 pKa = 6.91YY436 pKa = 10.77RR437 pKa = 11.84FSVANVSGPSTVTIDD452 pKa = 2.99IDD454 pKa = 3.34NSFRR458 pKa = 11.84MDD460 pKa = 4.56SIIEE464 pKa = 4.01LLDD467 pKa = 3.35SQGNVLASNDD477 pKa = 4.22DD478 pKa = 3.55GDD480 pKa = 4.62GGLDD484 pKa = 3.23PGSANIYY491 pKa = 10.69DD492 pKa = 3.83SFLTFTLPQNPATQTYY508 pKa = 8.02YY509 pKa = 10.33IRR511 pKa = 11.84VSTFSDD517 pKa = 3.42AGPASVFAGSSYY529 pKa = 9.97TLNVTANARR538 pKa = 11.84TPASGNILLGSRR550 pKa = 11.84LDD552 pKa = 4.05GGWGNDD558 pKa = 3.3QLIGGTGVDD567 pKa = 3.78TLIGGNGNDD576 pKa = 3.97TLTGGASADD585 pKa = 3.75LLYY588 pKa = 11.11GGSGDD593 pKa = 4.03DD594 pKa = 3.4NYY596 pKa = 10.53IVEE599 pKa = 4.54RR600 pKa = 11.84QDD602 pKa = 4.45DD603 pKa = 4.19LTFEE607 pKa = 4.66LLNSGVDD614 pKa = 3.41TVTASVGYY622 pKa = 9.28YY623 pKa = 10.14LYY625 pKa = 11.41AHH627 pKa = 7.14IEE629 pKa = 4.06NLTLADD635 pKa = 3.65GAGDD639 pKa = 3.37IFGVGNDD646 pKa = 4.15LNNVLTGNAGSNLMIGGLGHH666 pKa = 7.06DD667 pKa = 4.84VIRR670 pKa = 11.84GGAGVDD676 pKa = 3.38NLFGEE681 pKa = 4.53SGVDD685 pKa = 3.66QLFGDD690 pKa = 3.94AGIDD694 pKa = 3.63YY695 pKa = 10.53LVGGGGGDD703 pKa = 3.65MLDD706 pKa = 3.95GGADD710 pKa = 3.34ADD712 pKa = 3.7ALYY715 pKa = 11.03GEE717 pKa = 5.14EE718 pKa = 5.03EE719 pKa = 5.06DD720 pKa = 4.07DD721 pKa = 4.01TLIGGAGFHH730 pKa = 6.45TDD732 pKa = 2.8ILVGGTGDD740 pKa = 3.96DD741 pKa = 3.14MLYY744 pKa = 11.04ANSGLGDD751 pKa = 3.68YY752 pKa = 11.02DD753 pKa = 6.04LIDD756 pKa = 4.36GGPGNDD762 pKa = 3.32SYY764 pKa = 12.09YY765 pKa = 10.99VDD767 pKa = 4.26TPDD770 pKa = 5.16DD771 pKa = 3.83LTFEE775 pKa = 4.37ASGGGTDD782 pKa = 3.41TVYY785 pKa = 11.55ANINGAGYY793 pKa = 10.26YY794 pKa = 10.29LYY796 pKa = 11.04AHH798 pKa = 6.51TEE800 pKa = 3.94NLVLEE805 pKa = 4.77GNTPFGVGNEE815 pKa = 4.34LANQLTGNAIGNYY828 pKa = 9.52LLGGAGDD835 pKa = 4.21DD836 pKa = 4.23KK837 pKa = 11.86LNGKK841 pKa = 9.29AGNDD845 pKa = 3.37VLFGEE850 pKa = 5.42GGADD854 pKa = 2.85TFVFEE859 pKa = 5.1RR860 pKa = 11.84GTGGDD865 pKa = 3.59VIGDD869 pKa = 4.09FARR872 pKa = 11.84GTDD875 pKa = 4.31KK876 pKa = 10.89IDD878 pKa = 3.43LSAFGFANFAAVQAGFSQVGANGAINLGNGDD909 pKa = 4.95FIVLHH914 pKa = 6.05NVTMSQLAQGDD925 pKa = 4.75FILTATTPKK934 pKa = 9.98TEE936 pKa = 4.32SFGDD940 pKa = 3.8TAKK943 pKa = 10.75SAAIEE948 pKa = 4.04LADD951 pKa = 4.7AMRR954 pKa = 11.84DD955 pKa = 3.64APGAALDD962 pKa = 3.94WNPDD966 pKa = 2.38HH967 pKa = 7.47WMFHH971 pKa = 5.26YY972 pKa = 9.5QTGNNFGFAA981 pKa = 3.79

Molecular weight: 100.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8X191|A0A1P8X191_9SPHN Proprotein convertase P OS=Sphingopyxis sp. QXT-31 OX=1357916 GN=BWQ93_17610 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.43GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 9.1VLANRR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.26KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4007

0

4007

1276034

29

4048

318.5

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.941 ± 0.061

0.74 ± 0.011

6.201 ± 0.034

5.359 ± 0.035

3.575 ± 0.023

9.198 ± 0.046

1.935 ± 0.021

5.027 ± 0.024

3.137 ± 0.035

9.716 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.019

2.528 ± 0.031

5.369 ± 0.03

2.892 ± 0.023

6.999 ± 0.039

5.024 ± 0.028

5.181 ± 0.042

7.037 ± 0.032

1.499 ± 0.016

2.252 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski