Grapevine virus E

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3Y533|B3Y533_9VIRU Movement protein OS=Grapevine virus E OX=516956 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84RR3 pKa = 11.84LSGRR7 pKa = 11.84EE8 pKa = 3.85LVSSKK13 pKa = 11.17YY14 pKa = 10.59SLEE17 pKa = 3.68QLLFHH22 pKa = 6.94LNNYY26 pKa = 9.12ILDD29 pKa = 4.17GVVLPSEE36 pKa = 4.57LFNPLYY42 pKa = 9.27MVEE45 pKa = 4.16VEE47 pKa = 4.19EE48 pKa = 5.6SEE50 pKa = 4.42LLLVNQIKK58 pKa = 8.25WQKK61 pKa = 10.98SEE63 pKa = 3.99GQLLQSFALQQVGRR77 pKa = 11.84EE78 pKa = 4.06VAKK81 pKa = 10.75LSSFEE86 pKa = 4.17STPLCNIFLYY96 pKa = 10.92SDD98 pKa = 3.82FSGANLCEE106 pKa = 4.05LYY108 pKa = 10.76KK109 pKa = 10.82FLGCRR114 pKa = 11.84YY115 pKa = 9.84LDD117 pKa = 3.3SCYY120 pKa = 10.72VIEE123 pKa = 5.15AGSGSKK129 pKa = 10.29LVSSTNNSVQIGISPEE145 pKa = 3.72HH146 pKa = 5.82TAGILGKK153 pKa = 10.52LKK155 pKa = 9.51GVYY158 pKa = 10.28VIVEE162 pKa = 4.16EE163 pKa = 4.21LKK165 pKa = 8.86QTKK168 pKa = 9.64GVEE171 pKa = 4.02GPYY174 pKa = 9.16MFKK177 pKa = 10.62GRR179 pKa = 11.84PSPYY183 pKa = 10.09KK184 pKa = 10.0NAPPLPFF191 pKa = 5.3

Molecular weight: 21.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3Y535|B3Y535_9VIRU Putative nucleic acid binding protein OS=Grapevine virus E OX=516956 PE=4 SV=1
MM1 pKa = 7.77DD2 pKa = 4.4NKK4 pKa = 10.85YY5 pKa = 10.68LGEE8 pKa = 4.11SRR10 pKa = 11.84SAAKK14 pKa = 10.05RR15 pKa = 11.84RR16 pKa = 11.84AKK18 pKa = 10.37RR19 pKa = 11.84YY20 pKa = 8.23GRR22 pKa = 11.84CYY24 pKa = 8.55CCGRR28 pKa = 11.84IEE30 pKa = 4.59CNGDD34 pKa = 2.83VRR36 pKa = 11.84TTKK39 pKa = 10.69SQDD42 pKa = 3.24QVKK45 pKa = 9.64QAIRR49 pKa = 11.84TPAIRR54 pKa = 11.84FLTEE58 pKa = 3.57NEE60 pKa = 3.82GNYY63 pKa = 9.93INAAMNLALTDD74 pKa = 3.61MVKK77 pKa = 10.37LGKK80 pKa = 9.53SEE82 pKa = 4.13YY83 pKa = 10.18PFNFKK88 pKa = 10.49YY89 pKa = 10.76NKK91 pKa = 10.02APFGMRR97 pKa = 11.84SPEE100 pKa = 4.01EE101 pKa = 3.75TPEE104 pKa = 4.01YY105 pKa = 10.5YY106 pKa = 10.48NWW108 pKa = 3.45

Molecular weight: 12.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2461

108

1698

492.2

55.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.745 ± 0.967

2.113 ± 0.17

5.648 ± 1.4

7.111 ± 0.291

4.917 ± 0.286

6.542 ± 0.293

2.56 ± 0.649

5.689 ± 0.466

7.233 ± 0.717

10.037 ± 1.434

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.926 ± 0.315

4.226 ± 0.66

4.429 ± 0.545

3.169 ± 0.459

4.754 ± 0.51

6.705 ± 0.779

4.714 ± 0.496

6.136 ± 1.307

0.65 ± 0.227

3.698 ± 0.54
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski