Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) (Soil fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Sordariales; Chaetomiaceae; Chaetomium; Chaetomium globosum

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11042 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2HEQ2|Q2HEQ2_CHAGB Peptidyl-prolyl cis-trans isomerase OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=CHGG_01302 PE=3 SV=1
MM1 pKa = 7.14HH2 pKa = 7.3QYY4 pKa = 11.22ACLVSLLPLVAAHH17 pKa = 7.03GFLSSPPARR26 pKa = 11.84RR27 pKa = 11.84PGAAYY32 pKa = 9.83RR33 pKa = 11.84ATCGEE38 pKa = 3.96QPFYY42 pKa = 10.71QQSSDD47 pKa = 3.14INGNVQGIQQVVGSDD62 pKa = 3.05ATEE65 pKa = 4.24DD66 pKa = 3.76CNLWLCKK73 pKa = 10.52GFLLEE78 pKa = 5.45DD79 pKa = 4.14NPDD82 pKa = 3.47QVQSYY87 pKa = 11.22SLGQTIDD94 pKa = 2.98ITVNIAAPHH103 pKa = 5.52TGYY106 pKa = 11.49ANVSVVKK113 pKa = 9.97TSTNTMIGNPLIEE126 pKa = 4.37FQNYY130 pKa = 9.4ASNSGPGPPTTQPSVSPFRR149 pKa = 11.84KK150 pKa = 9.37AWAATAQPLATACSSGSGMPRR171 pKa = 11.84TLIRR175 pKa = 11.84PTRR178 pKa = 11.84PAPTSSWPLTGMGIALSSTSTVPVTTASAPATATSVDD215 pKa = 3.96PAPATTTTAVPGTPDD230 pKa = 4.07DD231 pKa = 4.25EE232 pKa = 5.45CPADD236 pKa = 4.09DD237 pKa = 5.67GGDD240 pKa = 3.56EE241 pKa = 5.57GEE243 pKa = 5.8DD244 pKa = 3.47EE245 pKa = 5.54DD246 pKa = 6.19DD247 pKa = 3.83EE248 pKa = 4.51QGEE251 pKa = 4.37SGEE254 pKa = 4.28GDD256 pKa = 3.54EE257 pKa = 4.81EE258 pKa = 5.27CPVDD262 pKa = 4.15GGEE265 pKa = 4.64EE266 pKa = 4.14PEE268 pKa = 4.62EE269 pKa = 5.75DD270 pKa = 5.26DD271 pKa = 6.77DD272 pKa = 5.92STDD275 pKa = 4.43DD276 pKa = 5.89DD277 pKa = 4.41NAEE280 pKa = 4.36CPADD284 pKa = 4.58DD285 pKa = 5.12GDD287 pKa = 3.95NAAATTSLGAKK298 pKa = 9.68DD299 pKa = 4.04VSTSAAATPTGSHH312 pKa = 6.43TSTVTQIVTSTVTTEE327 pKa = 3.9RR328 pKa = 11.84LVTVTAAATACNN340 pKa = 3.79

Molecular weight: 34.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2GSD5|Q2GSD5_CHAGB J domain-containing protein OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=CHGG_09119 PE=4 SV=1
MM1 pKa = 7.87PPPSPTTNQIRR12 pKa = 11.84PHH14 pKa = 5.97SAAPTAPQRR23 pKa = 11.84PPNATTATTGNSHH36 pKa = 7.14PRR38 pKa = 11.84PTISTATHH46 pKa = 4.5QTPSRR51 pKa = 11.84SGTPTTPQPPPNAIIATTGNNNNHH75 pKa = 6.23ARR77 pKa = 11.84PTTGTGNASGAHH89 pKa = 5.02TAVSRR94 pKa = 11.84QGTATPGRR102 pKa = 11.84NGTGAPSPPRR112 pKa = 11.84TNPAPGPNPPPGPGPNQTANLATRR136 pKa = 11.84SANGNGRR143 pKa = 11.84GGGAPGNGNGSATQQQAGGRR163 pKa = 11.84GARR166 pKa = 11.84RR167 pKa = 3.24

Molecular weight: 16.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11042

0

11042

5473786

20

9223

495.7

54.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.482 ± 0.021

1.24 ± 0.009

5.623 ± 0.016

6.092 ± 0.026

3.413 ± 0.013

7.624 ± 0.025

2.439 ± 0.011

4.183 ± 0.014

4.44 ± 0.02

8.689 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.072 ± 0.009

3.365 ± 0.011

6.715 ± 0.028

4.013 ± 0.018

6.781 ± 0.021

7.572 ± 0.024

6.07 ± 0.017

6.205 ± 0.019

1.495 ± 0.009

2.474 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski