Monilinia fructigena

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Monilinia

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10740 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A395IU60|A0A395IU60_9HELO Acetolactate synthase OS=Monilinia fructigena OX=38457 GN=DID88_003493 PE=3 SV=1
MM1 pKa = 7.13TKK3 pKa = 10.36VVMVNYY9 pKa = 10.08NYY11 pKa = 10.99LSIYY15 pKa = 10.17GNSTGALNGLALAGNIPVQASTTVNPQGFLNDD47 pKa = 4.43FYY49 pKa = 11.61DD50 pKa = 5.35DD51 pKa = 3.97EE52 pKa = 4.85GWWALGWIQAYY63 pKa = 10.07DD64 pKa = 3.37VTGNSDD70 pKa = 4.3YY71 pKa = 11.03LTMAEE76 pKa = 5.75DD77 pKa = 3.79IFADD81 pKa = 3.69MKK83 pKa = 10.93NASTTPCSKK92 pKa = 10.4RR93 pKa = 11.84GFGIWANDD101 pKa = 3.33RR102 pKa = 11.84DD103 pKa = 4.1SSNNGLSVNWAGPFVATANASTQSSALDD131 pKa = 3.59ALVAAAAIGVV141 pKa = 3.72

Molecular weight: 14.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A395IXE8|A0A395IXE8_9HELO RNase H domain-containing protein OS=Monilinia fructigena OX=38457 GN=DID88_001894 PE=4 SV=1
MM1 pKa = 7.64AHH3 pKa = 7.5PILPTPKK10 pKa = 10.02SPPQHH15 pKa = 6.55HH16 pKa = 6.68PHH18 pKa = 6.51NNQIHH23 pKa = 5.62NPARR27 pKa = 11.84ARR29 pKa = 11.84QPPTPPQPRR38 pKa = 11.84TLPPPNPGSRR48 pKa = 11.84NPNPNLTLQPLHH60 pKa = 7.19RR61 pKa = 11.84SRR63 pKa = 11.84PALPP67 pKa = 3.94

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10442

298

10740

4314917

66

4113

401.8

44.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.967 ± 0.024

1.151 ± 0.008

5.588 ± 0.017

6.595 ± 0.029

3.568 ± 0.015

6.715 ± 0.024

2.308 ± 0.01

5.457 ± 0.016

5.573 ± 0.026

8.467 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.162 ± 0.01

4.2 ± 0.012

5.86 ± 0.022

3.977 ± 0.019

5.921 ± 0.024

8.533 ± 0.036

6.192 ± 0.019

5.604 ± 0.015

1.383 ± 0.009

2.779 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski