Spiroplasma melliferum KC3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; Spiroplasma melliferum

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A037UU05|A0A037UU05_SPIME Adenine phosphoribosyltransferase OS=Spiroplasma melliferum KC3 OX=570509 GN=apt PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61VTTIILGTLGTIGSVIPAINTFSTNINPTTVDD34 pKa = 3.04NMKK37 pKa = 10.85NEE39 pKa = 4.64LDD41 pKa = 3.89NMDD44 pKa = 3.78SSLINSSKK52 pKa = 9.97MLEE55 pKa = 4.04EE56 pKa = 4.44LKK58 pKa = 10.7EE59 pKa = 4.3SSSSLMDD66 pKa = 4.04DD67 pKa = 3.5FQEE70 pKa = 3.99IFNAIMGEE78 pKa = 4.01DD79 pKa = 3.86DD80 pKa = 4.22SEE82 pKa = 4.21DD83 pKa = 3.89TIVEE87 pKa = 4.31TVEE90 pKa = 4.41GGITEE95 pKa = 4.95AGLEE99 pKa = 4.22SNIAEE104 pKa = 4.35TALEE108 pKa = 4.17SNIAEE113 pKa = 4.34TALEE117 pKa = 4.17SNIAEE122 pKa = 4.34TALEE126 pKa = 4.14SNIIGKK132 pKa = 7.02TVEE135 pKa = 4.27SGLLTSSLGTAATTIGVTVAIALAIYY161 pKa = 9.56GGYY164 pKa = 7.9YY165 pKa = 10.05TYY167 pKa = 9.71THH169 pKa = 7.11WDD171 pKa = 3.48DD172 pKa = 4.13VKK174 pKa = 10.25TFSNKK179 pKa = 9.18AVNDD183 pKa = 3.49VKK185 pKa = 10.93HH186 pKa = 6.24YY187 pKa = 10.87ISTTTDD193 pKa = 3.26YY194 pKa = 11.39LKK196 pKa = 10.97NWLFFF201 pKa = 4.29

Molecular weight: 21.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A037UQT8|A0A037UQT8_SPIME 30S ribosomal protein S14 type Z OS=Spiroplasma melliferum KC3 OX=570509 GN=rpsZ PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.14IKK11 pKa = 10.33HH12 pKa = 5.53KK13 pKa = 9.07RR14 pKa = 11.84THH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MEE23 pKa = 4.26SASGRR28 pKa = 11.84KK29 pKa = 9.01VLSKK33 pKa = 10.58RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.82GRR39 pKa = 11.84KK40 pKa = 8.62VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1154

0

1154

358577

44

1452

310.7

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.59 ± 0.081

0.736 ± 0.021

4.967 ± 0.056

5.809 ± 0.076

5.221 ± 0.078

5.131 ± 0.066

1.625 ± 0.027

9.643 ± 0.074

8.854 ± 0.079

9.94 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.167 ± 0.031

7.395 ± 0.095

3.014 ± 0.034

4.053 ± 0.053

3.062 ± 0.049

5.488 ± 0.05

5.863 ± 0.048

5.771 ± 0.06

1.281 ± 0.03

4.39 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski