Mycobacterium chubuense (strain NBB4)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium; Mycolicibacterium chubuense

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4BCH0|I4BCH0_MYCCN 2-epi-5-epi-valiolone synthase OS=Mycobacterium chubuense (strain NBB4) OX=710421 GN=Mycch_0151 PE=4 SV=1
MM1 pKa = 7.87TDD3 pKa = 2.63TDD5 pKa = 4.0YY6 pKa = 11.97KK7 pKa = 11.26LFVCVQCGFTYY18 pKa = 10.68DD19 pKa = 3.68EE20 pKa = 4.37AKK22 pKa = 10.31GWPEE26 pKa = 4.27DD27 pKa = 4.17GIAPGTRR34 pKa = 11.84WDD36 pKa = 5.68DD37 pKa = 4.13IPDD40 pKa = 3.72DD41 pKa = 4.45WSCPDD46 pKa = 3.66CGAAKK51 pKa = 10.31SDD53 pKa = 3.62FEE55 pKa = 4.6MIEE58 pKa = 3.97VARR61 pKa = 11.84PP62 pKa = 3.11

Molecular weight: 6.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4BDY2|I4BDY2_MYCCN Uncharacterized protein OS=Mycobacterium chubuense (strain NBB4) OX=710421 GN=Mycch_0671 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5779

0

5779

1892690

30

7413

327.5

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.245 ± 0.045

0.827 ± 0.009

6.342 ± 0.024

5.142 ± 0.032

2.998 ± 0.018

8.877 ± 0.035

2.243 ± 0.018

4.088 ± 0.022

2.103 ± 0.022

9.74 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.032 ± 0.014

2.116 ± 0.019

5.868 ± 0.03

2.889 ± 0.017

7.421 ± 0.032

5.457 ± 0.023

6.175 ± 0.037

8.82 ± 0.032

1.507 ± 0.014

2.111 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski