Sporosarcina psychrophila (Bacillus psychrophilus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Sporosarcina

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A127W4K7|A0A127W4K7_SPOPS ABC transporter substrate-binding protein OS=Sporosarcina psychrophila OX=1476 GN=AZE41_13810 PE=4 SV=1
MM1 pKa = 7.49SCNHH5 pKa = 6.29CVNSIEE11 pKa = 4.27TSVGEE16 pKa = 4.0LTGVSTVKK24 pKa = 10.37VDD26 pKa = 3.75LGNNEE31 pKa = 3.77VSVEE35 pKa = 3.91FDD37 pKa = 2.95NATTFAQIKK46 pKa = 7.23EE47 pKa = 4.48TIEE50 pKa = 3.83EE51 pKa = 3.73QGYY54 pKa = 9.62EE55 pKa = 4.11LVV57 pKa = 3.81

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A127VWA5|A0A127VWA5_SPOPS FAD-binding dehydrogenase OS=Sporosarcina psychrophila OX=1476 GN=AZE41_06375 PE=4 SV=1
MM1 pKa = 7.48ISVPSGRR8 pKa = 11.84FPRR11 pKa = 11.84ARR13 pKa = 11.84LQPPRR18 pKa = 11.84HH19 pKa = 6.02CVPAGSSACAVPAGVAALHH38 pKa = 5.7YY39 pKa = 8.71NQLVRR44 pKa = 11.84TIKK47 pKa = 10.47KK48 pKa = 9.91SFFSGFTRR56 pKa = 11.84SAEE59 pKa = 3.87AFLDD63 pKa = 3.6EE64 pKa = 5.42RR65 pKa = 11.84IAGFLPGEE73 pKa = 4.87KK74 pKa = 9.24PPKK77 pKa = 7.7MQFWRR82 pKa = 11.84LKK84 pKa = 10.29LSIGFSLLYY93 pKa = 10.65

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4219

0

4219

1221369

37

2030

289.5

32.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.413 ± 0.046

0.635 ± 0.011

5.288 ± 0.036

7.369 ± 0.043

4.614 ± 0.032

7.092 ± 0.037

1.948 ± 0.019

8.048 ± 0.037

6.49 ± 0.033

9.721 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.917 ± 0.017

4.212 ± 0.024

3.555 ± 0.021

3.358 ± 0.023

4.023 ± 0.034

6.065 ± 0.025

5.751 ± 0.029

7.209 ± 0.035

0.987 ± 0.014

3.304 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski