Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia; Pyricularia oryzae

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4MPU0|G4MPU0_MAGO7 Uncharacterized protein OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=MGG_16249 PE=4 SV=1
MM1 pKa = 7.57FSINKK6 pKa = 8.27VAFVAVGALAAVAGAQEE23 pKa = 4.71RR24 pKa = 11.84NTTDD28 pKa = 2.37ISSFPEE34 pKa = 3.94CAQWCVEE41 pKa = 4.17DD42 pKa = 3.75MQKK45 pKa = 10.2SEE47 pKa = 4.26KK48 pKa = 10.47ASEE51 pKa = 4.35LGCSDD56 pKa = 4.54GDD58 pKa = 4.4DD59 pKa = 3.76ACLCRR64 pKa = 11.84NANFAYY70 pKa = 9.91GVRR73 pKa = 11.84DD74 pKa = 4.68CSAQYY79 pKa = 11.2CNDD82 pKa = 3.26RR83 pKa = 11.84TTGLDD88 pKa = 3.28AIKK91 pKa = 10.75YY92 pKa = 8.94VFDD95 pKa = 3.49WCDD98 pKa = 3.26KK99 pKa = 10.93EE100 pKa = 5.48GVQLSSVVSAGATNATGIATPTGAAGGAGSASASTSPVTTAEE142 pKa = 4.21YY143 pKa = 9.52TSTMTTDD150 pKa = 3.18GSTVTTTGFTTVTPSATASGVVSTITSDD178 pKa = 2.74GSTFVSTIASSASDD192 pKa = 3.62MVSTITSDD200 pKa = 2.62GSTFVSTIAASASNAVSTIVSDD222 pKa = 3.93GSTFVSTLTDD232 pKa = 3.11AAGSVTSRR240 pKa = 11.84AGDD243 pKa = 3.52AASSATDD250 pKa = 3.34AAGTSATAEE259 pKa = 4.07GFAPQMTAAPAGILALAGVAAFLLL283 pKa = 4.38

Molecular weight: 27.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4N422|G4N422_MAGO7 Uncharacterized protein OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=MGG_16716 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12676

116

12792

5652975

34

6541

441.9

48.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.434 ± 0.027

1.27 ± 0.01

5.741 ± 0.016

5.895 ± 0.024

3.49 ± 0.016

7.438 ± 0.022

2.311 ± 0.009

4.364 ± 0.016

4.721 ± 0.02

8.503 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.009

3.512 ± 0.013

6.328 ± 0.027

4.063 ± 0.019

6.4 ± 0.022

8.144 ± 0.022

5.886 ± 0.021

6.286 ± 0.019

1.423 ± 0.009

2.521 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski