Fibrella aestuarina BUZ 2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Fibrella; Fibrella aestuarina

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5627 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0K1V9|I0K1V9_9BACT Transcriptional repressor CopY family OS=Fibrella aestuarina BUZ 2 OX=1166018 GN=FAES_0098 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.3KK3 pKa = 10.6VILTGSALLAFVGAAWAQNSATLNQSGTYY32 pKa = 6.93QTAVQQQSGNNQVSTINQNQGAGVNTGNWAGTFQTGTVTNTATINQRR79 pKa = 11.84TGSQGNRR86 pKa = 11.84AAVGMVGGSGNVGTINQNGGALGLSGGATPPPGAGFDD123 pKa = 3.57QAVGDD128 pKa = 4.26GNFGGILQLGNTNTGVIINQNNLSQRR154 pKa = 11.84NTGEE158 pKa = 3.82IYY160 pKa = 10.81QNGDD164 pKa = 2.82NNANTTISQSNNSVGNQSMILQGFTSRR191 pKa = 11.84DD192 pKa = 3.29VASQVSSNNQATVEE206 pKa = 4.05QNGDD210 pKa = 3.05GTLAGSSSGNRR221 pKa = 11.84ADD223 pKa = 3.51VRR225 pKa = 11.84QLGEE229 pKa = 3.86QHH231 pKa = 7.05VSTTQQNGGSGGSSIGNTVSVDD253 pKa = 3.29QSGFGQTASVQQNGTANGSSTGNTATVVQSAFTNQATVEE292 pKa = 4.08QNGRR296 pKa = 11.84SEE298 pKa = 4.73GNTASVTQQGSTGGEE313 pKa = 4.04VPTLIGGGNTASVYY327 pKa = 10.5QNGGVAGLSAGNQATVSQDD346 pKa = 3.04GDD348 pKa = 3.43QHH350 pKa = 5.95TATVYY355 pKa = 10.66QGDD358 pKa = 4.11GTGGGTFSSEE368 pKa = 3.85ANTATVTQTGQTQTATIYY386 pKa = 8.43QQPGADD392 pKa = 3.67GSSALNTATITQSEE406 pKa = 4.81VEE408 pKa = 4.17NTATISQNNASVSNNATILQSGSASGVANNASIKK442 pKa = 10.8QNDD445 pKa = 3.67LSLGQTALVTQAGTNLFANVEE466 pKa = 4.33QNGITGGFTSSGNSATVTQTGDD488 pKa = 2.99FHH490 pKa = 7.0TVFAEE495 pKa = 4.07QNAGGNGGSINNQITITQVGVTGAHH520 pKa = 5.67NAEE523 pKa = 4.52VSQSDD528 pKa = 3.19ASGFNQITVDD538 pKa = 3.89QSGTSQQAFVNQSGVAFGIAAGSLGNQAVVTQSGTSNFADD578 pKa = 5.41LYY580 pKa = 9.59QTDD583 pKa = 3.71AAQDD587 pKa = 3.72GVITLTQSGNSNSAQLYY604 pKa = 8.19QVAGVTSNTATVEE617 pKa = 4.05QSGTTGIAAIQQIYY631 pKa = 10.86GSDD634 pKa = 3.34QANATILQSGTTNNAQIYY652 pKa = 8.78QYY654 pKa = 10.63QGTGNSVVIDD664 pKa = 3.64QLTTGTNNYY673 pKa = 10.19AEE675 pKa = 5.01IYY677 pKa = 10.39QGDD680 pKa = 4.22DD681 pKa = 2.73SFAPDD686 pKa = 3.45GSDD689 pKa = 3.58FNTTTVTQRR698 pKa = 11.84GTFNTVRR705 pKa = 11.84FDD707 pKa = 5.55LIGDD711 pKa = 3.81NNTATMSQTGRR722 pKa = 11.84GNLIRR727 pKa = 11.84GVAGTSPFSGYY738 pKa = 10.64AGQYY742 pKa = 10.68GNGNTLTITQVSDD755 pKa = 3.07AAGPGQTASVYY766 pKa = 9.96QAGNNNTTTITQTNNN781 pKa = 2.55

Molecular weight: 78.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0K8P0|I0K8P0_9BACT Hydrolase or acyltransferase (Alpha/beta hydrolase superfamily)-like protein OS=Fibrella aestuarina BUZ 2 OX=1166018 GN=FAES_2484 PE=4 SV=1
MM1 pKa = 7.32NVNTLTAPPRR11 pKa = 11.84SRR13 pKa = 11.84PAEE16 pKa = 3.84PVVTLHH22 pKa = 6.68RR23 pKa = 11.84PWLMPVLTGGLLLTSLLSVGVGAVAISPAEE53 pKa = 3.76VLAILGHH60 pKa = 5.4SLGLTGAVDD69 pKa = 3.38EE70 pKa = 4.72TKK72 pKa = 10.88AVILTGIRR80 pKa = 11.84LPRR83 pKa = 11.84VCLGLLIGAGLAVAGAAIQGLFRR106 pKa = 11.84NPLADD111 pKa = 4.41PGLIGISSGASLAAVGMIVLEE132 pKa = 4.03VTLFQKK138 pKa = 10.97LSGLLGFYY146 pKa = 10.63ALSLVAFGGACGTTLLVYY164 pKa = 10.55RR165 pKa = 11.84LARR168 pKa = 11.84VAGRR172 pKa = 11.84SVVTTMLLAGIAINALAGALTGLLTYY198 pKa = 10.68LATDD202 pKa = 3.53DD203 pKa = 3.71QLRR206 pKa = 11.84TITFWALGSLGGATWTTVLTILPFTIVVLLGLPRR240 pKa = 11.84LAKK243 pKa = 10.35SLNLLALGEE252 pKa = 4.37SQAAMLGVHH261 pKa = 5.24ITGLKK266 pKa = 9.71RR267 pKa = 11.84RR268 pKa = 11.84VIVLATLAVGTSVAVAGIIGFIGLVIPHH296 pKa = 7.81LIRR299 pKa = 11.84LVAGSDD305 pKa = 3.19HH306 pKa = 6.69RR307 pKa = 11.84RR308 pKa = 11.84VLTGSALGGAIVLTAADD325 pKa = 3.71ALARR329 pKa = 11.84TIVAPAEE336 pKa = 4.04LPIGILTALLGTPVFLWMLIKK357 pKa = 10.43EE358 pKa = 4.32RR359 pKa = 11.84STTT362 pKa = 3.4

Molecular weight: 36.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5627

0

5627

2040039

29

4965

362.5

40.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.254 ± 0.037

0.747 ± 0.01

5.409 ± 0.026

4.748 ± 0.028

4.182 ± 0.023

7.436 ± 0.033

1.812 ± 0.018

5.096 ± 0.028

4.242 ± 0.031

10.357 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.054 ± 0.016

4.433 ± 0.029

4.959 ± 0.025

4.623 ± 0.025

5.851 ± 0.026

5.817 ± 0.032

6.859 ± 0.046

7.009 ± 0.024

1.362 ± 0.014

3.748 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski