Methylobacterium sp. 17Sr1-43

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4920 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8VYZ9|A0A2U8VYZ9_9RHIZ Putative membrane protein insertion efficiency factor OS=Methylobacterium sp. 17Sr1-43 OX=2202828 GN=DK427_14665 PE=3 SV=1
MM1 pKa = 7.79PATFGTATTTLVEE14 pKa = 4.24SEE16 pKa = 4.03PWEE19 pKa = 4.45LSAADD24 pKa = 4.13LNGDD28 pKa = 3.67GKK30 pKa = 11.33LDD32 pKa = 3.47LVVPNTASNSLSVLIGNGDD51 pKa = 3.52GSFRR55 pKa = 11.84PAVNYY60 pKa = 8.21PVSSSPISGALADD73 pKa = 3.65VTGDD77 pKa = 3.47GKK79 pKa = 10.64IDD81 pKa = 3.29IVVGINGAPSLLAGNGDD98 pKa = 3.84GTFQAAAGIAGGNALQSYY116 pKa = 7.12WLRR119 pKa = 11.84AVDD122 pKa = 4.87LNADD126 pKa = 3.79GKK128 pKa = 11.42LDD130 pKa = 3.79LAYY133 pKa = 9.96TNFGPNQLVVQYY145 pKa = 10.94GNGDD149 pKa = 3.6GTFGTGALYY158 pKa = 10.66SVGNLPRR165 pKa = 11.84APAIGDD171 pKa = 3.65FNHH174 pKa = 7.23DD175 pKa = 3.52GKK177 pKa = 11.43LDD179 pKa = 3.41IVTANSGATSVSVLLANADD198 pKa = 3.33GTYY201 pKa = 10.24QSQVPYY207 pKa = 9.99PVGASPYY214 pKa = 10.39SVATGDD220 pKa = 3.89FNGDD224 pKa = 3.14GNLDD228 pKa = 3.77LVTAALNANSYY239 pKa = 10.94SVLLGRR245 pKa = 11.84GDD247 pKa = 3.8GTFQPALTTAAQGNSNEE264 pKa = 4.06ITVADD269 pKa = 3.54VDD271 pKa = 4.22GDD273 pKa = 4.03GKK275 pKa = 11.2LDD277 pKa = 4.58LIIANSSANQVGIAYY292 pKa = 8.89GQGDD296 pKa = 4.18GTFSAWQTLPTGGGPYY312 pKa = 9.1TAIAVDD318 pKa = 4.05LNGDD322 pKa = 3.41GRR324 pKa = 11.84LDD326 pKa = 3.54LAVTNRR332 pKa = 11.84VSGTVTTYY340 pKa = 8.83MQSTPPTLSITGATVVEE357 pKa = 4.96GNSGTADD364 pKa = 3.11AAFVVTRR371 pKa = 11.84SDD373 pKa = 3.48TTGSPTATYY382 pKa = 9.2TITNSTTDD390 pKa = 3.35GADD393 pKa = 3.5FGPGFQATGTVTFAAGATTATINVPIRR420 pKa = 11.84GDD422 pKa = 3.52TLYY425 pKa = 10.57EE426 pKa = 3.92ASEE429 pKa = 4.49TFTVALSNPQGATISGTGQATGTITNDD456 pKa = 3.36DD457 pKa = 4.11APPTVTLSGGTSIAEE472 pKa = 4.42GNSGTTVVPITATLSAVSGLATTVTLAFGGTATQGTDD509 pKa = 3.32YY510 pKa = 9.81TVSSTTITIPAGSLSGSVNVNVLGDD535 pKa = 3.65TAVEE539 pKa = 4.16PNGTATVTIATATNATPAGAAQTVTIQNDD568 pKa = 3.65DD569 pKa = 3.0VSYY572 pKa = 11.17AITASAAAVAEE583 pKa = 4.65GSSGAGNAVTYY594 pKa = 9.13TVTRR598 pKa = 11.84TGDD601 pKa = 3.17MTQAGVATVRR611 pKa = 11.84LSGEE615 pKa = 3.81ATRR618 pKa = 11.84GDD620 pKa = 4.46DD621 pKa = 4.38YY622 pKa = 11.55STTLPANGQINFVAGDD638 pKa = 3.53ASKK641 pKa = 9.48TFTVTTVPDD650 pKa = 3.57TTVEE654 pKa = 3.91PNEE657 pKa = 4.07RR658 pKa = 11.84VIATITGVSGQGGATIGTPASATTTITNDD687 pKa = 3.13DD688 pKa = 3.75QYY690 pKa = 11.49GVSITATDD698 pKa = 3.47ASKK701 pKa = 11.53LEE703 pKa = 4.62GNTGTTPFTFTVSRR717 pKa = 11.84TGGTADD723 pKa = 5.52AITLAYY729 pKa = 10.16AVTGSGASAADD740 pKa = 3.37AADD743 pKa = 3.84FGGTLPSGTVTIAAGQASTTLMINVTGDD771 pKa = 3.65GLVEE775 pKa = 4.82ADD777 pKa = 4.89DD778 pKa = 4.8GFTVTLSNATANATIVQSTAIGTIRR803 pKa = 11.84NDD805 pKa = 3.56DD806 pKa = 3.85QPPYY810 pKa = 9.62TPPTAPTAVADD821 pKa = 4.06TGSVTLGTALTKK833 pKa = 10.4AAPGVLANDD842 pKa = 3.88SGAGATLTVTAVNGQAANVGKK863 pKa = 10.15ALAGAYY869 pKa = 10.02GNLTLNADD877 pKa = 3.71GSYY880 pKa = 10.76SYY882 pKa = 11.67APDD885 pKa = 3.24FAKK888 pKa = 10.6AVFTGSVVDD897 pKa = 3.81HH898 pKa = 6.31FTYY901 pKa = 10.8TDD903 pKa = 3.37TANGQTATTSLDD915 pKa = 3.14IAVAPPSAATLALFGTVVTNPASQTGGIYY944 pKa = 10.73GLYY947 pKa = 8.79TALLNRR953 pKa = 11.84VPDD956 pKa = 3.98ALGLEE961 pKa = 4.58GFSAAIQAGSDD972 pKa = 3.47LTTVASALLGSAEE985 pKa = 4.15RR986 pKa = 11.84GGTVSDD992 pKa = 3.42PTTYY996 pKa = 10.84VQGLYY1001 pKa = 11.02ANALHH1006 pKa = 6.13RR1007 pKa = 11.84TADD1010 pKa = 3.37AGGLNFFVNEE1020 pKa = 3.95LNAGVSQATVAVQIATSSEE1039 pKa = 3.98AQSLNTPAFQRR1050 pKa = 11.84GVFVTDD1056 pKa = 5.03AIDD1059 pKa = 3.51AGVARR1064 pKa = 11.84LYY1066 pKa = 10.98YY1067 pKa = 10.66GLLNRR1072 pKa = 11.84SPDD1075 pKa = 3.44AGGLGSFEE1083 pKa = 4.43GLVKK1087 pKa = 10.23QAAASGAAAGAIQGLATVANVMLGSPEE1114 pKa = 3.77FAATHH1119 pKa = 6.72AGQTSAAYY1127 pKa = 10.27VDD1129 pKa = 3.93SLYY1132 pKa = 11.25VGALGRR1138 pKa = 11.84HH1139 pKa = 6.1ADD1141 pKa = 3.52AAGASYY1147 pKa = 10.32FGAEE1151 pKa = 4.13LAQGVSRR1158 pKa = 11.84ATVALQIVEE1167 pKa = 4.19SAEE1170 pKa = 4.13AQVHH1174 pKa = 4.62LVGVIEE1180 pKa = 5.23QGFQLTAA1187 pKa = 3.68

Molecular weight: 117.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8VM32|A0A2U8VM32_9RHIZ Uncharacterized protein OS=Methylobacterium sp. 17Sr1-43 OX=2202828 GN=DK427_01670 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.12VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 4.95GRR39 pKa = 11.84KK40 pKa = 9.25RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4920

0

4920

1526070

29

2669

310.2

33.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.602 ± 0.062

0.844 ± 0.01

5.458 ± 0.024

5.691 ± 0.035

3.303 ± 0.023

9.18 ± 0.033

1.915 ± 0.017

4.206 ± 0.029

2.366 ± 0.031

10.542 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.994 ± 0.015

2.055 ± 0.019

5.909 ± 0.034

2.773 ± 0.019

8.483 ± 0.04

4.656 ± 0.021

5.164 ± 0.027

7.612 ± 0.028

1.233 ± 0.015

2.012 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski