Pelagibaca abyssi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacte

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8UWP2|A0A1P8UWP2_9RHOB Phage protein OS=Pelagibaca abyssi OX=1250539 GN=Ga0080574_TMP3422 PE=4 SV=1
MM1 pKa = 7.82TITNDD6 pKa = 2.85NDD8 pKa = 3.65VIFGSGEE15 pKa = 4.35GYY17 pKa = 10.51FGRR20 pKa = 11.84SGTNPVSTVLSDD32 pKa = 3.6GRR34 pKa = 11.84IAVAWNEE41 pKa = 3.76YY42 pKa = 9.86QYY44 pKa = 10.94PGDD47 pKa = 3.83GSIPGIDD54 pKa = 3.64FDD56 pKa = 3.49TWTRR60 pKa = 11.84ILNADD65 pKa = 3.7GTPATEE71 pKa = 4.04AMLVNDD77 pKa = 4.07TRR79 pKa = 11.84VGSHH83 pKa = 6.48ASAEE87 pKa = 4.08ITALSDD93 pKa = 2.89GGYY96 pKa = 9.25VIGWITMNRR105 pKa = 11.84ATVEE109 pKa = 4.12GGTQTVYY116 pKa = 10.44TYY118 pKa = 10.21DD119 pKa = 2.91AHH121 pKa = 6.64VRR123 pKa = 11.84SFTAAGAPEE132 pKa = 4.75GPAVLVSPDD141 pKa = 3.52LEE143 pKa = 4.65TYY145 pKa = 10.85DD146 pKa = 3.75PADD149 pKa = 3.68YY150 pKa = 11.39NSILASNIEE159 pKa = 3.95NVNIVSLAAGGAVVIYY175 pKa = 8.43EE176 pKa = 3.98YY177 pKa = 10.79RR178 pKa = 11.84GGGSGYY184 pKa = 10.62SYY186 pKa = 11.25GGTHH190 pKa = 6.61ARR192 pKa = 11.84IVGNDD197 pKa = 3.71GQSLGEE203 pKa = 3.96PVRR206 pKa = 11.84VWEE209 pKa = 4.81GGLFNLDD216 pKa = 3.15AVQLSNGDD224 pKa = 3.62LAFVEE229 pKa = 4.95FAGDD233 pKa = 3.59LSGYY237 pKa = 8.41RR238 pKa = 11.84VRR240 pKa = 11.84LSAADD245 pKa = 3.89LTSAPATIAGASGPVVIDD263 pKa = 4.56HH264 pKa = 7.6DD265 pKa = 4.19PQPDD269 pKa = 3.56QNAGGYY275 pKa = 10.07GSAPRR280 pKa = 11.84IAALSDD286 pKa = 2.96GGFAVLYY293 pKa = 9.77GYY295 pKa = 10.22NVQLGADD302 pKa = 4.01DD303 pKa = 5.44DD304 pKa = 4.32EE305 pKa = 5.04SLRR308 pKa = 11.84IDD310 pKa = 4.06RR311 pKa = 11.84FDD313 pKa = 3.5AQGGYY318 pKa = 10.0QSTVSIPVPDD328 pKa = 4.49DD329 pKa = 3.29TVQQPGAIPYY339 pKa = 8.78EE340 pKa = 3.98ILEE343 pKa = 4.15LSGNRR348 pKa = 11.84LLVAWSHH355 pKa = 4.46VVAYY359 pKa = 10.41GDD361 pKa = 3.66TDD363 pKa = 3.08IMAVVINADD372 pKa = 3.49GSLDD376 pKa = 3.63SAPAVINPNTANYY389 pKa = 9.75QLLGDD394 pKa = 4.57LTALPDD400 pKa = 3.68GDD402 pKa = 4.39VFMSLADD409 pKa = 3.56TSGVQVGGVADD420 pKa = 3.82YY421 pKa = 9.99MHH423 pKa = 7.22GLFLGMPEE431 pKa = 4.54DD432 pKa = 4.41PGANTPVVRR441 pKa = 11.84IGTAGDD447 pKa = 3.51DD448 pKa = 3.76RR449 pKa = 11.84LVGTANNDD457 pKa = 3.56TLRR460 pKa = 11.84GLDD463 pKa = 3.91GNDD466 pKa = 3.24TLEE469 pKa = 4.62GRR471 pKa = 11.84DD472 pKa = 4.12GDD474 pKa = 4.04DD475 pKa = 3.35QLFGGDD481 pKa = 4.93DD482 pKa = 3.66NDD484 pKa = 4.54LVLGGDD490 pKa = 4.45GSDD493 pKa = 3.22TMAGDD498 pKa = 4.2GGNDD502 pKa = 3.45TLRR505 pKa = 11.84GGNSAADD512 pKa = 4.03LRR514 pKa = 11.84DD515 pKa = 3.69VIYY518 pKa = 10.74GGAGDD523 pKa = 4.9DD524 pKa = 5.51DD525 pKa = 4.46IDD527 pKa = 4.39GGYY530 pKa = 11.11GNDD533 pKa = 4.01EE534 pKa = 4.14LRR536 pKa = 11.84GDD538 pKa = 4.22AGNDD542 pKa = 3.85TIVGGYY548 pKa = 9.71GADD551 pKa = 3.37TVIGGTGNDD560 pKa = 3.66ALTGQTWSDD569 pKa = 3.87AIFGGDD575 pKa = 3.55GMDD578 pKa = 5.06FINGGFGHH586 pKa = 7.05DD587 pKa = 3.55RR588 pKa = 11.84VNGGTGADD596 pKa = 3.08RR597 pKa = 11.84FYY599 pKa = 11.0HH600 pKa = 6.38LGVEE604 pKa = 4.6GHH606 pKa = 6.22GSDD609 pKa = 4.17WIQDD613 pKa = 3.69FSDD616 pKa = 3.79EE617 pKa = 4.33EE618 pKa = 5.54GDD620 pKa = 3.79LLVFGGTGAAVEE632 pKa = 4.15DD633 pKa = 4.24FQVNFTEE640 pKa = 4.42TANAGAAGVEE650 pKa = 4.33EE651 pKa = 4.43TFVIYY656 pKa = 10.48RR657 pKa = 11.84PTGQILWALVDD668 pKa = 4.72GGAQDD673 pKa = 5.28EE674 pKa = 5.07IMLQLDD680 pKa = 3.58GMSYY684 pKa = 11.5DD685 pKa = 4.04LLAA688 pKa = 5.6

Molecular weight: 71.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8UVF7|A0A1P8UVF7_9RHOB Uncharacterized protein OS=Pelagibaca abyssi OX=1250539 GN=Ga0080574_TMP3028 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44AGRR28 pKa = 11.84KK29 pKa = 8.46VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.51QLSAA44 pKa = 3.97

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5048

0

5048

1524221

37

5509

301.9

32.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.814 ± 0.053

0.878 ± 0.012

5.932 ± 0.041

6.359 ± 0.039

3.577 ± 0.019

8.949 ± 0.045

2.009 ± 0.018

4.864 ± 0.025

2.808 ± 0.032

10.249 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.021

2.322 ± 0.021

5.258 ± 0.029

3.046 ± 0.017

7.092 ± 0.041

5.1 ± 0.028

5.308 ± 0.033

7.119 ± 0.028

1.39 ± 0.014

2.202 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski