Bacillus phage BC-T25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Tsarbombavirus; unclassified Tsarbombavirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9MUQ5|A0A5B9MUQ5_9CAUD Uncharacterized protein OS=Bacillus phage BC-T25 OX=2596657 PE=4 SV=1
MM1 pKa = 7.45TIIHH5 pKa = 6.22SEE7 pKa = 3.8NGILNGEE14 pKa = 4.06GSGLIVEE21 pKa = 5.42FINRR25 pKa = 11.84YY26 pKa = 8.36EE27 pKa = 4.54SIHH30 pKa = 5.5GTWKK34 pKa = 10.31AYY36 pKa = 9.31EE37 pKa = 4.43FYY39 pKa = 10.08TSEE42 pKa = 4.61EE43 pKa = 3.86ITFQTFDD50 pKa = 3.29SAGNLHH56 pKa = 7.19DD57 pKa = 4.75EE58 pKa = 4.38VSSTIFRR65 pKa = 11.84LEE67 pKa = 3.47IGEE70 pKa = 4.44GYY72 pKa = 10.27EE73 pKa = 3.9AVLIANHH80 pKa = 7.04DD81 pKa = 4.26EE82 pKa = 4.58YY83 pKa = 11.28CVHH86 pKa = 7.01EE87 pKa = 4.56DD88 pKa = 2.76VSYY91 pKa = 11.6AFDD94 pKa = 3.27VDD96 pKa = 4.76KK97 pKa = 10.92IVSQMKK103 pKa = 9.68EE104 pKa = 3.88IEE106 pKa = 4.09SSIPDD111 pKa = 3.35VQEE114 pKa = 4.99DD115 pKa = 4.18YY116 pKa = 11.77NEE118 pKa = 4.12LNEE121 pKa = 4.08EE122 pKa = 4.13DD123 pKa = 4.3EE124 pKa = 4.99EE125 pKa = 5.88GDD127 pKa = 3.61EE128 pKa = 4.38YY129 pKa = 11.87

Molecular weight: 14.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9MVE1|A0A5B9MVE1_9CAUD Uncharacterized protein OS=Bacillus phage BC-T25 OX=2596657 PE=4 SV=1
MM1 pKa = 6.41TTRR4 pKa = 11.84FRR6 pKa = 11.84WKK8 pKa = 10.27GSCKK12 pKa = 9.24GFKK15 pKa = 10.42GEE17 pKa = 3.97RR18 pKa = 11.84HH19 pKa = 6.2KK20 pKa = 10.96EE21 pKa = 3.94RR22 pKa = 11.84QRR24 pKa = 11.84EE25 pKa = 4.21KK26 pKa = 10.19YY27 pKa = 9.9RR28 pKa = 11.84KK29 pKa = 8.56ALEE32 pKa = 3.93VTIEE36 pKa = 4.58RR37 pKa = 11.84IQGEE41 pKa = 4.62TVPIEE46 pKa = 4.22HH47 pKa = 6.86IRR49 pKa = 11.84NLRR52 pKa = 11.84GAQQ55 pKa = 3.18

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

236

0

236

47419

36

1451

200.9

22.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.639 ± 0.212

0.81 ± 0.066

5.915 ± 0.122

7.864 ± 0.274

3.914 ± 0.11

6.535 ± 0.252

1.856 ± 0.116

6.348 ± 0.149

8.073 ± 0.203

8.066 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.904 ± 0.094

5.287 ± 0.172

3.326 ± 0.145

3.927 ± 0.134

4.549 ± 0.134

5.629 ± 0.169

6.107 ± 0.241

6.847 ± 0.16

1.154 ± 0.071

4.251 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski