Mycobacterium sp. 1164966.3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A0MAS0|A0A1A0MAS0_9MYCO S-adenosyl-L-methionine-dependent methyltransferase OS=Mycobacterium sp. 1164966.3 OX=1856861 GN=A9W99_10715 PE=3 SV=1
MM1 pKa = 7.53SFLTAQPEE9 pKa = 4.18EE10 pKa = 4.1LAAAAGKK17 pKa = 10.42LGVIGSAFAAQNAAAAAPTTGVIPAAADD45 pKa = 3.47EE46 pKa = 4.24VSILQASLFAAYY58 pKa = 7.37GTLYY62 pKa = 10.35QQVSGDD68 pKa = 3.4ATAMYY73 pKa = 10.63DD74 pKa = 3.6SFVNTLQTSAGTYY87 pKa = 7.67EE88 pKa = 3.97ATEE91 pKa = 4.14AANTSAAASPLSSVGAAAAPAAAADD116 pKa = 4.07PPGLDD121 pKa = 3.03IADD124 pKa = 3.55IVDD127 pKa = 3.15IGGGNWASAASNFLGLANGGLLVPAEE153 pKa = 4.35QGTGTAGAALLGFDD167 pKa = 3.92GASAEE172 pKa = 4.35SAATAGLGEE181 pKa = 4.33VPAAGMGPAFAGMGVASSVGALSAPASWAGSTTLVSSTATPSPGVSPGAGWTAAAPPAASATLLPGMPGYY251 pKa = 10.72AAVGRR256 pKa = 11.84NSAGFGAPRR265 pKa = 11.84YY266 pKa = 8.08GVKK269 pKa = 10.02PIVMPKK275 pKa = 9.47PAAGG279 pKa = 3.33

Molecular weight: 26.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A0MA46|A0A1A0MA46_9MYCO NLPC_P60 domain-containing protein OS=Mycobacterium sp. 1164966.3 OX=1856861 GN=A9W99_11880 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84SIVSGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.5GRR42 pKa = 11.84RR43 pKa = 11.84VLSAA47 pKa = 3.76

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4963

0

4963

1635646

29

7479

329.6

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.822 ± 0.05

0.861 ± 0.01

6.191 ± 0.028

5.327 ± 0.031

3.07 ± 0.02

8.669 ± 0.033

2.269 ± 0.015

4.282 ± 0.022

2.301 ± 0.024

9.847 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.014

2.3 ± 0.016

5.869 ± 0.034

3.144 ± 0.018

7.421 ± 0.034

5.476 ± 0.022

5.796 ± 0.023

8.533 ± 0.031

1.533 ± 0.014

2.201 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski