Wenzhou tombus-like virus 11

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG22|A0A1L3KG22_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 11 OX=1923664 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.17GRR4 pKa = 11.84PISDD8 pKa = 3.31RR9 pKa = 11.84QLPGSSLRR17 pKa = 11.84TGEE20 pKa = 4.53CVHH23 pKa = 5.84SCRR26 pKa = 11.84RR27 pKa = 11.84YY28 pKa = 6.76TRR30 pKa = 11.84KK31 pKa = 9.17MFDD34 pKa = 3.12YY35 pKa = 9.18DD36 pKa = 3.46TDD38 pKa = 4.01FGGSDD43 pKa = 3.7SVVKK47 pKa = 8.86THH49 pKa = 7.1LSCVCNEE56 pKa = 3.63VLALKK61 pKa = 10.15NRR63 pKa = 11.84HH64 pKa = 5.37QLDD67 pKa = 3.46DD68 pKa = 3.69GARR71 pKa = 11.84YY72 pKa = 7.06TFKK75 pKa = 11.24GDD77 pKa = 3.01LTKK80 pKa = 10.1WVRR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84VKK87 pKa = 10.55PMTPWSEE94 pKa = 4.05DD95 pKa = 3.14MVIEE99 pKa = 4.53HH100 pKa = 7.77AIPSKK105 pKa = 10.84KK106 pKa = 9.74KK107 pKa = 10.12LLMSAKK113 pKa = 9.72EE114 pKa = 4.03SLKK117 pKa = 10.48QYY119 pKa = 10.95PLTEE123 pKa = 3.99KK124 pKa = 10.72DD125 pKa = 3.22AAIKK129 pKa = 10.18MFLKK133 pKa = 10.27DD134 pKa = 3.75DD135 pKa = 4.15KK136 pKa = 11.57YY137 pKa = 10.58HH138 pKa = 8.41GEE140 pKa = 4.16MKK142 pKa = 10.46DD143 pKa = 3.45PRR145 pKa = 11.84CIQYY149 pKa = 10.48RR150 pKa = 11.84NKK152 pKa = 10.47RR153 pKa = 11.84YY154 pKa = 9.5NLRR157 pKa = 11.84LGTYY161 pKa = 7.56LHH163 pKa = 6.77PLEE166 pKa = 5.0EE167 pKa = 4.74YY168 pKa = 10.78VMSWTHH174 pKa = 6.52RR175 pKa = 11.84GTHH178 pKa = 5.9IFAKK182 pKa = 9.05GRR184 pKa = 11.84NMRR187 pKa = 11.84QRR189 pKa = 11.84GRR191 pKa = 11.84DD192 pKa = 2.96IAAKK196 pKa = 10.13FNGIEE201 pKa = 4.09DD202 pKa = 4.31CVVLSIDD209 pKa = 3.37HH210 pKa = 6.77SKK212 pKa = 10.66FDD214 pKa = 3.75CHH216 pKa = 7.09VNRR219 pKa = 11.84QLLEE223 pKa = 4.23AEE225 pKa = 3.72HH226 pKa = 6.61WFYY229 pKa = 11.34NQCFGSEE236 pKa = 3.93EE237 pKa = 4.18LAFLLQLQIRR247 pKa = 11.84NKK249 pKa = 9.2GTTKK253 pKa = 10.64NGTRR257 pKa = 11.84YY258 pKa = 5.91TTINTRR264 pKa = 11.84MSGDD268 pKa = 3.38KK269 pKa = 9.66NTGLGNSLINYY280 pKa = 7.38MMIKK284 pKa = 9.4QVLMEE289 pKa = 4.32LAIKK293 pKa = 9.79HH294 pKa = 5.92NYY296 pKa = 9.27YY297 pKa = 10.04IDD299 pKa = 5.02GDD301 pKa = 3.96DD302 pKa = 3.88SNVFVQRR309 pKa = 11.84RR310 pKa = 11.84FAHH313 pKa = 5.83LVKK316 pKa = 10.81AEE318 pKa = 3.98LFSRR322 pKa = 11.84FGMVTKK328 pKa = 10.21IEE330 pKa = 4.12NVADD334 pKa = 4.27VIEE337 pKa = 5.63HH338 pKa = 7.37IDD340 pKa = 3.57FCQCRR345 pKa = 11.84PVFDD349 pKa = 3.98GSGYY353 pKa = 8.93TMVRR357 pKa = 11.84NPEE360 pKa = 3.78RR361 pKa = 11.84MLARR365 pKa = 11.84LPWVVGPIEE374 pKa = 4.16EE375 pKa = 4.65SRR377 pKa = 11.84ALDD380 pKa = 3.08ITYY383 pKa = 8.13ATGQCEE389 pKa = 3.91IAQGLGLPIGQYY401 pKa = 9.48IGQRR405 pKa = 11.84MCEE408 pKa = 3.98LGGKK412 pKa = 7.34MVRR415 pKa = 11.84LRR417 pKa = 11.84HH418 pKa = 5.49RR419 pKa = 11.84AWLEE423 pKa = 3.5KK424 pKa = 9.79MKK426 pKa = 10.25PGKK429 pKa = 9.5LQPIEE434 pKa = 3.78PAAGVRR440 pKa = 11.84EE441 pKa = 4.45SYY443 pKa = 11.19ALAWGLSVADD453 pKa = 3.82QLAIEE458 pKa = 4.44QTRR461 pKa = 11.84LVQPEE466 pKa = 4.02EE467 pKa = 4.79LYY469 pKa = 10.7TPGTLEE475 pKa = 3.86WLL477 pKa = 3.91

Molecular weight: 54.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG58|A0A1L3KG58_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 11 OX=1923664 PE=4 SV=1
MM1 pKa = 7.18SQPGSRR7 pKa = 11.84GQSRR11 pKa = 11.84GAGRR15 pKa = 11.84QTPNSGHH22 pKa = 7.17RR23 pKa = 11.84GRR25 pKa = 11.84DD26 pKa = 3.41GPRR29 pKa = 11.84RR30 pKa = 11.84PTGDD34 pKa = 3.48DD35 pKa = 3.06RR36 pKa = 11.84QPNQRR41 pKa = 11.84ARR43 pKa = 11.84GQHH46 pKa = 5.74PSGNARR52 pKa = 11.84GPQWQTVGRR61 pKa = 11.84RR62 pKa = 11.84PRR64 pKa = 11.84AANPEE69 pKa = 3.63ALMRR73 pKa = 11.84RR74 pKa = 11.84IHH76 pKa = 7.14ALEE79 pKa = 4.39QINKK83 pKa = 9.79DD84 pKa = 3.59LLIQLGRR91 pKa = 11.84INPGEE96 pKa = 4.21GSSGSTSRR104 pKa = 11.84GSTSGKK110 pKa = 8.58ATPEE114 pKa = 4.09KK115 pKa = 9.09PTPAPRR121 pKa = 11.84KK122 pKa = 9.56GPEE125 pKa = 4.01SRR127 pKa = 11.84NPTPVHH133 pKa = 5.46QASTATPRR141 pKa = 11.84GEE143 pKa = 4.08NQASRR148 pKa = 11.84TSKK151 pKa = 10.11GRR153 pKa = 11.84QRR155 pKa = 11.84CRR157 pKa = 11.84TLSNSHH163 pKa = 7.01LPRR166 pKa = 11.84VTTPNPFEE174 pKa = 4.12LLEE177 pKa = 4.19EE178 pKa = 4.44EE179 pKa = 5.03FPHH182 pKa = 7.13LSPGTLALAASVGSSGACTPTVKK205 pKa = 9.9TKK207 pKa = 10.59SGAPAGPSRR216 pKa = 11.84RR217 pKa = 11.84GRR219 pKa = 11.84SPPSTQRR226 pKa = 11.84ASSLPALNAQHH237 pKa = 6.9RR238 pKa = 11.84APRR241 pKa = 11.84AGGQQPSRR249 pKa = 11.84TFVTSSTPTQGTSSQRR265 pKa = 11.84PGATAEE271 pKa = 4.25PKK273 pKa = 9.91VAPNQAGSDD282 pKa = 3.8PAPQPRR288 pKa = 11.84VRR290 pKa = 11.84FRR292 pKa = 11.84PGDD295 pKa = 3.67FGWRR299 pKa = 11.84GAAQHH304 pKa = 6.37GVFHH308 pKa = 7.05SPTVASPRR316 pKa = 11.84YY317 pKa = 9.93AGACLEE323 pKa = 4.3ASNKK327 pKa = 9.76KK328 pKa = 10.55GPLANDD334 pKa = 3.58SNPVFILKK342 pKa = 10.3PEE344 pKa = 3.7VRR346 pKa = 11.84TDD348 pKa = 3.4VEE350 pKa = 4.58CGVGSTRR357 pKa = 11.84ANLARR362 pKa = 11.84LLDD365 pKa = 4.36LKK367 pKa = 11.08EE368 pKa = 4.0MSLDD372 pKa = 3.79SLYY375 pKa = 10.67QDD377 pKa = 4.46SGLDD381 pKa = 3.54PNSWKK386 pKa = 10.75DD387 pKa = 3.58LVSFTIDD394 pKa = 2.81KK395 pKa = 10.98AKK397 pKa = 10.9ALVVDD402 pKa = 3.7EE403 pKa = 4.29FMYY406 pKa = 11.15YY407 pKa = 10.45EE408 pKa = 4.21LVSRR412 pKa = 11.84FPFTVRR418 pKa = 11.84TADD421 pKa = 3.85LARR424 pKa = 11.84KK425 pKa = 7.79MFNHH429 pKa = 6.69LNILMKK435 pKa = 10.77QFDD438 pKa = 3.93TRR440 pKa = 11.84MYY442 pKa = 9.2TAKK445 pKa = 9.88QLYY448 pKa = 9.19RR449 pKa = 11.84IKK451 pKa = 10.54QATVRR456 pKa = 11.84AALLPPAEE464 pKa = 4.24EE465 pKa = 4.01LLTRR469 pKa = 11.84KK470 pKa = 10.23LIQQEE475 pKa = 4.17SKK477 pKa = 11.0EE478 pKa = 4.11MKK480 pKa = 9.87KK481 pKa = 10.96YY482 pKa = 10.7NAFAEE487 pKa = 4.31KK488 pKa = 10.59GNAGSVFAPGAGIVAAATSLAQTRR512 pKa = 11.84VGLSPDD518 pKa = 3.05KK519 pKa = 10.89KK520 pKa = 10.76

Molecular weight: 55.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1399

402

520

466.3

51.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.72 ± 1.368

1.43 ± 0.467

4.718 ± 0.572

4.861 ± 0.591

3.717 ± 0.494

7.863 ± 0.445

2.073 ± 0.456

3.574 ± 0.858

6.004 ± 0.468

8.149 ± 0.561

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.716 ± 0.63

3.788 ± 0.12

6.505 ± 1.534

5.075 ± 0.375

8.077 ± 0.735

7.577 ± 1.181

6.934 ± 0.728

5.575 ± 0.55

1.072 ± 0.284

2.573 ± 0.68
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski