Flammulina velutipes browning virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0STM1|C0STM1_9VIRU Coat protein OS=Flammulina velutipes browning virus OX=568090 GN=CP1 PE=4 SV=1
MM1 pKa = 7.5SPPRR5 pKa = 11.84FPKK8 pKa = 10.6PPVTPSASQTADD20 pKa = 3.31PLGTRR25 pKa = 11.84DD26 pKa = 3.47VDD28 pKa = 4.19SEE30 pKa = 4.41SSKK33 pKa = 10.9PKK35 pKa = 10.22LVNSRR40 pKa = 11.84PQKK43 pKa = 9.6AVPRR47 pKa = 11.84AGPTSAPSVSDD58 pKa = 3.59ANPIYY63 pKa = 10.37HH64 pKa = 6.69AVSNFPYY71 pKa = 10.27YY72 pKa = 9.8PHH74 pKa = 6.53QRR76 pKa = 11.84LDD78 pKa = 2.94TSTFVPCTQVLFAVLAHH95 pKa = 6.78MDD97 pKa = 3.9NIMCTTYY104 pKa = 10.5SWTQSQPAWHH114 pKa = 6.94PCVSRR119 pKa = 11.84LYY121 pKa = 10.59FSTLVYY127 pKa = 9.96YY128 pKa = 10.22QILRR132 pKa = 11.84AMDD135 pKa = 4.09AARR138 pKa = 11.84IITVLQKK145 pKa = 10.76EE146 pKa = 4.26LLNFLEE152 pKa = 4.3HH153 pKa = 6.99TIPASALPIPGPLAPFLRR171 pKa = 11.84SPFLLFNWHH180 pKa = 6.01GWLLQCMSNHH190 pKa = 5.76TXWPHH195 pKa = 5.0HH196 pKa = 5.97QKK198 pKa = 9.99IKK200 pKa = 10.74YY201 pKa = 9.14NISSAFNNLLPNLVHH216 pKa = 6.95AVDD219 pKa = 3.7SAYY222 pKa = 11.3SFATDD227 pKa = 2.86ATYY230 pKa = 11.23VFRR233 pKa = 11.84SAVNDD238 pKa = 3.81LNVKK242 pKa = 9.39MEE244 pKa = 4.21ATDD247 pKa = 3.77PDD249 pKa = 3.69MPAAEE254 pKa = 4.07TRR256 pKa = 11.84HH257 pKa = 5.8WNSAMMDD264 pKa = 3.55PVYY267 pKa = 10.65RR268 pKa = 11.84NDD270 pKa = 3.03MYY272 pKa = 11.1IPTRR276 pKa = 11.84VAQNFAAFSAPLGLPLRR293 pKa = 11.84YY294 pKa = 9.81NYY296 pKa = 10.87GPDD299 pKa = 3.32TDD301 pKa = 4.0IGLSHH306 pKa = 7.55FLYY309 pKa = 10.87LSGGKK314 pKa = 9.0HH315 pKa = 7.0DD316 pKa = 4.29YY317 pKa = 11.07LNRR320 pKa = 11.84LAPVFARR327 pKa = 11.84YY328 pKa = 8.6SQYY331 pKa = 10.66FVNSKK336 pKa = 10.14SLQEE340 pKa = 4.04CSPVGSAPGQINGTLTDD357 pKa = 5.83AIPVPTISGTTVYY370 pKa = 10.22TPSTLAALVTPWSSTTIYY388 pKa = 9.47THH390 pKa = 6.58VNLSLPEE397 pKa = 4.07FFEE400 pKa = 4.38HH401 pKa = 6.5QAALTNVNVTEE412 pKa = 4.73HH413 pKa = 6.32GAAPTFGTTATTLNGSFWRR432 pKa = 11.84MKK434 pKa = 8.42PHH436 pKa = 5.8YY437 pKa = 9.82RR438 pKa = 11.84EE439 pKa = 4.18SPPINVTKK447 pKa = 10.8NLFNIISNEE456 pKa = 3.72MFAEE460 pKa = 4.44PTSS463 pKa = 3.57

Molecular weight: 51.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0STM1|C0STM1_9VIRU Coat protein OS=Flammulina velutipes browning virus OX=568090 GN=CP1 PE=4 SV=1
MM1 pKa = 7.98SDD3 pKa = 3.18TLIDD7 pKa = 3.62SFSRR11 pKa = 11.84LTLSVKK17 pKa = 10.51NFVFLGFTEE26 pKa = 4.42TQNYY30 pKa = 6.17PQKK33 pKa = 10.49SDD35 pKa = 3.36SAILSHH41 pKa = 7.05RR42 pKa = 11.84KK43 pKa = 9.01VVLNAFEE50 pKa = 5.31KK51 pKa = 9.95YY52 pKa = 10.58LNPIEE57 pKa = 4.29YY58 pKa = 10.05NHH60 pKa = 6.39VANEE64 pKa = 4.12YY65 pKa = 10.18KK66 pKa = 10.51RR67 pKa = 11.84SEE69 pKa = 4.17TDD71 pKa = 3.2LDD73 pKa = 3.65STKK76 pKa = 11.05AAFFKK81 pKa = 11.1GDD83 pKa = 3.59IPDD86 pKa = 3.96HH87 pKa = 5.48EE88 pKa = 4.79VPRR91 pKa = 11.84DD92 pKa = 3.42EE93 pKa = 4.91HH94 pKa = 6.29YY95 pKa = 11.37NRR97 pKa = 11.84AFSVIVSKK105 pKa = 10.24FRR107 pKa = 11.84PPEE110 pKa = 4.18PIRR113 pKa = 11.84PVHH116 pKa = 5.64YY117 pKa = 10.57ADD119 pKa = 4.27LRR121 pKa = 11.84LYY123 pKa = 9.13PWPLKK128 pKa = 10.38PSAEE132 pKa = 4.1APFSNDD138 pKa = 2.51KK139 pKa = 11.13SLLALLALRR148 pKa = 11.84NRR150 pKa = 11.84QGFLPNAKK158 pKa = 9.95PNFHH162 pKa = 6.89NLFNWVFGFNRR173 pKa = 11.84QCVHH177 pKa = 7.27LIKK180 pKa = 10.45KK181 pKa = 10.36GKK183 pKa = 10.15DD184 pKa = 3.05NLGPNEE190 pKa = 4.09YY191 pKa = 9.46WPAHH195 pKa = 5.43GFLYY199 pKa = 9.93PINIHH204 pKa = 5.41TKK206 pKa = 8.42SAIIGIHH213 pKa = 6.96DD214 pKa = 4.12PNKK217 pKa = 8.91VRR219 pKa = 11.84TIFGVPKK226 pKa = 8.81LTVMVEE232 pKa = 4.99AMFFWPLFRR241 pKa = 11.84YY242 pKa = 9.79YY243 pKa = 10.41RR244 pKa = 11.84FEE246 pKa = 4.03QQSPLLWGYY255 pKa = 7.77EE256 pKa = 3.97TMLGGWYY263 pKa = 10.2KK264 pKa = 10.9LNHH267 pKa = 6.16EE268 pKa = 4.2LHH270 pKa = 6.88LNPFYY275 pKa = 10.62QGSILSLDD283 pKa = 3.0WSFFDD288 pKa = 4.21GRR290 pKa = 11.84ALFSVINDD298 pKa = 4.34LYY300 pKa = 10.78SDD302 pKa = 4.35KK303 pKa = 11.06GVKK306 pKa = 9.85SYY308 pKa = 11.62FEE310 pKa = 4.37FNNGYY315 pKa = 9.4IPTVDD320 pKa = 4.01YY321 pKa = 10.39PDD323 pKa = 4.81SSTHH327 pKa = 5.11PQKK330 pKa = 10.98LHH332 pKa = 6.56NLWDD336 pKa = 3.49WMLTALKK343 pKa = 9.73FAPCALADD351 pKa = 3.58GTIWQRR357 pKa = 11.84TVRR360 pKa = 11.84GIASGQFTTQFMDD373 pKa = 4.03SIYY376 pKa = 10.5NGLMILTILSRR387 pKa = 11.84MGFVIDD393 pKa = 3.33EE394 pKa = 4.23TLPIKK399 pKa = 10.76LLGDD403 pKa = 3.87DD404 pKa = 3.73SVTRR408 pKa = 11.84LAVSIPASMHH418 pKa = 5.53EE419 pKa = 4.1SFLIEE424 pKa = 4.04FQRR427 pKa = 11.84LADD430 pKa = 3.73YY431 pKa = 10.67YY432 pKa = 10.78FSHH435 pKa = 7.49TINVKK440 pKa = 9.87KK441 pKa = 10.7SKK443 pKa = 10.32ISNTPHH449 pKa = 5.76NVSVLSYY456 pKa = 10.93ANNNGLPVRR465 pKa = 11.84SRR467 pKa = 11.84TSLLCALLYY476 pKa = 9.77PKK478 pKa = 10.08SRR480 pKa = 11.84RR481 pKa = 11.84PTWEE485 pKa = 3.6HH486 pKa = 5.45LKK488 pKa = 10.75ARR490 pKa = 11.84AIGVYY495 pKa = 9.59YY496 pKa = 10.29ASCGIDD502 pKa = 2.83RR503 pKa = 11.84TVRR506 pKa = 11.84LICKK510 pKa = 10.15DD511 pKa = 2.74IFDD514 pKa = 4.53YY515 pKa = 11.25LDD517 pKa = 3.41SQGIQASSAGLQDD530 pKa = 5.16LFDD533 pKa = 5.61PNFKK537 pKa = 10.7SGTIPLDD544 pKa = 3.18VFPSVEE550 pKa = 3.99QVTTNLRR557 pKa = 11.84SFHH560 pKa = 6.83HH561 pKa = 7.06LDD563 pKa = 3.38NSDD566 pKa = 3.38KK567 pKa = 10.75EE568 pKa = 4.72RR569 pKa = 11.84YY570 pKa = 9.21FPTSHH575 pKa = 7.46FLDD578 pKa = 4.01TKK580 pKa = 11.01

Molecular weight: 66.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1043

463

580

521.5

59.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.999 ± 1.388

0.959 ± 0.081

5.081 ± 0.8

3.164 ± 0.383

6.136 ± 0.783

4.219 ± 0.511

3.643 ± 0.019

4.89 ± 0.671

4.219 ± 1.09

9.779 ± 0.764

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.013 ± 0.388

5.944 ± 0.069

7.862 ± 1.389

2.972 ± 0.179

4.506 ± 0.414

8.629 ± 0.296

6.711 ± 1.292

5.753 ± 0.342

1.822 ± 0.063

4.602 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski