Lactobacillus phage ATCC 8014-B2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Tybeckvirinae; Douglaswolinvirus; Lactobacillus virus B2

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4I4D1|K4I4D1_9CAUD Uncharacterized protein OS=Lactobacillus phage ATCC 8014-B2 OX=1225795 GN=8014-B2_0048 PE=4 SV=1
MM1 pKa = 7.02TANKK5 pKa = 9.75KK6 pKa = 9.2FQEE9 pKa = 4.05LLNNGEE15 pKa = 4.29LTDD18 pKa = 3.92EE19 pKa = 4.24QQEE22 pKa = 3.89LAYY25 pKa = 9.9QYY27 pKa = 11.6GVIDD31 pKa = 3.8VNSLYY36 pKa = 10.68EE37 pKa = 4.09YY38 pKa = 10.32LTDD41 pKa = 3.77TLSSEE46 pKa = 4.23SFEE49 pKa = 4.49DD50 pKa = 3.16SGRR53 pKa = 11.84VIEE56 pKa = 5.61LLNEE60 pKa = 4.07LLKK63 pKa = 10.59HH64 pKa = 6.69DD65 pKa = 4.48SNDD68 pKa = 3.28YY69 pKa = 11.05VLYY72 pKa = 10.72DD73 pKa = 3.94DD74 pKa = 6.04DD75 pKa = 6.28KK76 pKa = 11.38ISEE79 pKa = 4.5LDD81 pKa = 3.26YY82 pKa = 10.49DD83 pKa = 4.31TIEE86 pKa = 6.04DD87 pKa = 3.72ILNN90 pKa = 3.58

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4I0B3|K4I0B3_9CAUD Uncharacterized protein OS=Lactobacillus phage ATCC 8014-B2 OX=1225795 GN=8014-B2_001 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 9.92LSKK5 pKa = 9.92VAKK8 pKa = 9.09VVMAIVAGVMLSVMVINTKK27 pKa = 10.71AEE29 pKa = 3.89ASKK32 pKa = 10.84PNYY35 pKa = 9.82AYY37 pKa = 11.06SLVRR41 pKa = 11.84TKK43 pKa = 10.26QHH45 pKa = 5.05TVKK48 pKa = 9.84LTNTGKK54 pKa = 8.6TEE56 pKa = 3.44NMYY59 pKa = 10.55RR60 pKa = 11.84ITVKK64 pKa = 10.27QGKK67 pKa = 9.24HH68 pKa = 3.76STWYY72 pKa = 10.63YY73 pKa = 8.03MALIAKK79 pKa = 8.24QSWTVKK85 pKa = 10.08QSGKK89 pKa = 10.33YY90 pKa = 8.55SVTVRR95 pKa = 11.84RR96 pKa = 11.84ISKK99 pKa = 10.47ADD101 pKa = 3.53EE102 pKa = 4.1KK103 pKa = 11.29RR104 pKa = 11.84NADD107 pKa = 3.61PRR109 pKa = 11.84NHH111 pKa = 6.3FVPQGISNTQRR122 pKa = 11.84TVWNRR127 pKa = 2.9

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

23151

32

1887

182.3

20.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.125 ± 0.471

0.799 ± 0.116

7.015 ± 0.38

6.367 ± 0.355

3.909 ± 0.171

6.764 ± 0.389

1.728 ± 0.133

6.782 ± 0.206

8.142 ± 0.318

8.242 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.118

6.492 ± 0.202

2.51 ± 0.167

3.287 ± 0.176

3.339 ± 0.157

7.455 ± 0.327

5.978 ± 0.276

6.432 ± 0.228

1.184 ± 0.114

4.704 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski