Lysobacter spongiicola DSM 21749

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; Lysobacter spongiicola

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2864 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4SEU7|A0A1T4SEU7_9GAMM Quinoprotein glucose dehydrogenase OS=Lysobacter spongiicola DSM 21749 OX=1122188 GN=SAMN02745674_02798 PE=3 SV=1
MM1 pKa = 7.61NNTQITALAAACLFGLAACGNDD23 pKa = 3.9APPASDD29 pKa = 4.26PPVAPATTTAPDD41 pKa = 3.69TASTDD46 pKa = 3.73APPTAATPADD56 pKa = 4.04AGTATPAASAPAPADD71 pKa = 3.27NGKK74 pKa = 8.86PAAVVNDD81 pKa = 3.92CSTTIEE87 pKa = 4.83GNDD90 pKa = 3.02AMQFDD95 pKa = 4.64VGSITVPSSCSEE107 pKa = 3.69FTITLEE113 pKa = 4.24HH114 pKa = 6.31TGQMPVAAMGHH125 pKa = 5.22NVVISKK131 pKa = 10.39AADD134 pKa = 3.67RR135 pKa = 11.84EE136 pKa = 4.45AVAQAGMSAGVDD148 pKa = 3.22GDD150 pKa = 4.1YY151 pKa = 11.31VDD153 pKa = 5.27PNDD156 pKa = 3.8EE157 pKa = 3.8RR158 pKa = 11.84VIAHH162 pKa = 6.22TEE164 pKa = 4.17LIGGGEE170 pKa = 4.32STSVTFPVSDD180 pKa = 3.4IQGDD184 pKa = 4.04GPYY187 pKa = 10.67EE188 pKa = 4.07FFCSFPGHH196 pKa = 5.66WAVMRR201 pKa = 11.84GSIQVGG207 pKa = 3.26

Molecular weight: 20.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4RFJ2|A0A1T4RFJ2_9GAMM Uncharacterized protein OS=Lysobacter spongiicola DSM 21749 OX=1122188 GN=SAMN02745674_02151 PE=4 SV=1
MM1 pKa = 7.05ATKK4 pKa = 10.25KK5 pKa = 10.11AAKK8 pKa = 9.45KK9 pKa = 10.34KK10 pKa = 9.34PAAKK14 pKa = 10.2KK15 pKa = 8.51ATKK18 pKa = 9.93KK19 pKa = 10.29VAAKK23 pKa = 10.14KK24 pKa = 9.33PAAKK28 pKa = 9.9KK29 pKa = 9.11VAKK32 pKa = 10.19KK33 pKa = 10.31SAAKK37 pKa = 10.24KK38 pKa = 8.95PAAKK42 pKa = 10.15KK43 pKa = 9.96AVKK46 pKa = 10.29KK47 pKa = 9.74PAAKK51 pKa = 10.18KK52 pKa = 9.83AVKK55 pKa = 10.24KK56 pKa = 10.71SATKK60 pKa = 8.95KK61 pKa = 7.26TAKK64 pKa = 10.18KK65 pKa = 9.09ATAKK69 pKa = 10.42KK70 pKa = 9.95SAAKK74 pKa = 10.13KK75 pKa = 9.5SASKK79 pKa = 10.61KK80 pKa = 9.03SASKK84 pKa = 8.98KK85 pKa = 7.49TATRR89 pKa = 11.84KK90 pKa = 6.5TAKK93 pKa = 10.09KK94 pKa = 9.07ATAKK98 pKa = 10.54KK99 pKa = 9.36PAAKK103 pKa = 9.98KK104 pKa = 9.52AAKK107 pKa = 8.56KK108 pKa = 7.15TAKK111 pKa = 10.28KK112 pKa = 9.23ATAKK116 pKa = 10.4KK117 pKa = 8.61ATARR121 pKa = 11.84KK122 pKa = 8.93SAAKK126 pKa = 10.03KK127 pKa = 8.48STARR131 pKa = 11.84KK132 pKa = 8.16PAAKK136 pKa = 8.69KK137 pKa = 7.07TAKK140 pKa = 9.85KK141 pKa = 10.38AAVKK145 pKa = 10.51KK146 pKa = 9.69PAAKK150 pKa = 9.91KK151 pKa = 9.93AASKK155 pKa = 10.76KK156 pKa = 9.13PAAKK160 pKa = 9.81KK161 pKa = 9.93ASRR164 pKa = 11.84RR165 pKa = 11.84SAKK168 pKa = 9.94KK169 pKa = 9.38QAAPTAMPATPAPMII184 pKa = 4.39

Molecular weight: 18.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2864

0

2864

938805

39

2158

327.8

35.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.681 ± 0.057

0.799 ± 0.013

6.029 ± 0.033

6.065 ± 0.044

3.255 ± 0.026

8.953 ± 0.044

2.29 ± 0.022

3.99 ± 0.031

2.446 ± 0.036

10.643 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.019

2.298 ± 0.024

5.513 ± 0.038

3.46 ± 0.026

7.885 ± 0.042

5.236 ± 0.034

4.793 ± 0.029

7.702 ± 0.039

1.499 ± 0.022

2.142 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski