Alishewanella agri BL06

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Alishewanella; Alishewanella agri

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I8U6L0|I8U6L0_9ALTE Type IV pilus biogenesis protein C OS=Alishewanella agri BL06 OX=1195246 GN=AGRI_09220 PE=3 SV=1
MM1 pKa = 7.66SDD3 pKa = 2.9NRR5 pKa = 11.84LFLLYY10 pKa = 10.49DD11 pKa = 3.69PSFDD15 pKa = 3.65EE16 pKa = 5.31MDD18 pKa = 4.19AEE20 pKa = 4.7GCPGFGYY27 pKa = 10.91VLLFSEE33 pKa = 4.46QDD35 pKa = 3.29AEE37 pKa = 4.34QFTPGEE43 pKa = 4.27NPSCAAVSMLFTDD56 pKa = 4.14HH57 pKa = 7.45ADD59 pKa = 3.47GSISGDD65 pKa = 3.51LLGWAHH71 pKa = 7.23LDD73 pKa = 3.4DD74 pKa = 5.27PVFQRR79 pKa = 11.84YY80 pKa = 8.25PLGHH84 pKa = 6.77FLALMEE90 pKa = 4.14QAAQVAINAYY100 pKa = 8.95RR101 pKa = 11.84QVHH104 pKa = 4.88QVPDD108 pKa = 3.53KK109 pKa = 11.08LVAVHH114 pKa = 6.98LADD117 pKa = 6.24DD118 pKa = 4.95DD119 pKa = 4.94LLQFDD124 pKa = 4.46VQFLDD129 pKa = 4.76LQLTDD134 pKa = 3.74QQAEE138 pKa = 4.33TQLAQQLMLGRR149 pKa = 11.84PYY151 pKa = 10.98LDD153 pKa = 3.24SS154 pKa = 4.09

Molecular weight: 17.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I9P1B5|I9P1B5_9ALTE EAL domain-containing protein OS=Alishewanella agri BL06 OX=1195246 GN=AGRI_10006 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.46RR13 pKa = 11.84KK14 pKa = 9.37RR15 pKa = 11.84NHH17 pKa = 5.37GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.4NGRR29 pKa = 11.84QVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.91GRR40 pKa = 11.84KK41 pKa = 9.04RR42 pKa = 11.84LTAA45 pKa = 4.18

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3152

0

3152

1056891

37

1608

335.3

37.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.666 ± 0.051

0.956 ± 0.017

4.918 ± 0.037

5.427 ± 0.036

3.943 ± 0.027

6.688 ± 0.042

2.056 ± 0.021

5.308 ± 0.033

4.103 ± 0.035

12.226 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.02

3.509 ± 0.032

4.415 ± 0.028

6.386 ± 0.061

5.27 ± 0.03

6.006 ± 0.032

4.981 ± 0.026

6.644 ± 0.037

1.34 ± 0.022

2.978 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski