Microbacterium phage Roman

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 116 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514U2S1|A0A514U2S1_9CAUD Uncharacterized protein OS=Microbacterium phage Roman OX=2593341 GN=8 PE=4 SV=1
MM1 pKa = 7.58AVTHH5 pKa = 5.54NTIEE9 pKa = 4.34GAPGIPMIIEE19 pKa = 4.34SPDD22 pKa = 3.5TFPFAPGEE30 pKa = 4.1RR31 pKa = 11.84DD32 pKa = 4.07SEE34 pKa = 4.19WHH36 pKa = 6.55DD37 pKa = 3.53TTDD40 pKa = 3.67AEE42 pKa = 4.6YY43 pKa = 10.79AVYY46 pKa = 9.82AAYY49 pKa = 9.29WDD51 pKa = 4.1SQDD54 pKa = 4.2GAFDD58 pKa = 3.94PAPEE62 pKa = 3.98GWEE65 pKa = 4.06DD66 pKa = 3.97YY67 pKa = 10.48EE68 pKa = 4.42LVSAAQHH75 pKa = 5.96AANPHH80 pKa = 5.43RR81 pKa = 11.84FEE83 pKa = 6.68GEE85 pKa = 3.86LL86 pKa = 3.52

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514U2T9|A0A514U2T9_9CAUD Uncharacterized protein OS=Microbacterium phage Roman OX=2593341 GN=24 PE=4 SV=1
MM1 pKa = 7.56SVIEE5 pKa = 4.32FKK7 pKa = 10.75NARR10 pKa = 11.84GQVRR14 pKa = 11.84YY15 pKa = 9.8QPAHH19 pKa = 6.13PDD21 pKa = 2.96GRR23 pKa = 11.84RR24 pKa = 11.84LYY26 pKa = 11.13SGGNWMLPADD36 pKa = 4.0VDD38 pKa = 3.15AWAYY42 pKa = 10.1QPSLYY47 pKa = 9.76RR48 pKa = 11.84SKK50 pKa = 10.51RR51 pKa = 11.84RR52 pKa = 11.84ASRR55 pKa = 11.84RR56 pKa = 11.84VSWLIAEE63 pKa = 4.69EE64 pKa = 4.17FNAAQSQFKK73 pKa = 10.34AVAKK77 pKa = 10.48

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

116

0

116

19262

33

1252

166.1

18.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.983 ± 0.383

0.519 ± 0.104

6.526 ± 0.223

7.471 ± 0.362

3.354 ± 0.13

8.135 ± 0.318

1.864 ± 0.159

5.249 ± 0.133

4.672 ± 0.288

7.029 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.393 ± 0.152

3.847 ± 0.238

4.356 ± 0.24

3.51 ± 0.166

6.604 ± 0.207

6.007 ± 0.283

6.1 ± 0.242

7.17 ± 0.182

2.035 ± 0.151

3.177 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski