Serpula lacrymans var. lacrymans (strain S7.3) (Dry rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Boletales; Coniophorineae; Serpulaceae; Serpula; Serpula lacrymans; Serpula lacrymans var. lacrymans

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14339 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8Q7Z7|F8Q7Z7_SERL3 Zn(2)-C6 fungal-type domain-containing protein OS=Serpula lacrymans var. lacrymans (strain S7.3) OX=936435 GN=SERLA73DRAFT_59903 PE=4 SV=1
MM1 pKa = 6.86STEE4 pKa = 4.22SFDD7 pKa = 4.1FLHH10 pKa = 7.08LARR13 pKa = 11.84SKK15 pKa = 10.88LHH17 pKa = 5.41TAIGGKK23 pKa = 9.17DD24 pKa = 3.12NCSLHH29 pKa = 6.6RR30 pKa = 11.84WVLLKK35 pKa = 10.93NSIVRR40 pKa = 11.84TPPKK44 pKa = 10.39DD45 pKa = 3.77GEE47 pKa = 4.67VQNPMPDD54 pKa = 3.39VNSVYY59 pKa = 11.02SFDD62 pKa = 5.53DD63 pKa = 3.75EE64 pKa = 6.21GDD66 pKa = 3.75GDD68 pKa = 4.76DD69 pKa = 4.52EE70 pKa = 6.6DD71 pKa = 6.48DD72 pKa = 4.24GVEE75 pKa = 4.0EE76 pKa = 5.04EE77 pKa = 4.72IDD79 pKa = 3.52SFMFPDD85 pKa = 4.02ASNLVDD91 pKa = 4.98CGGADD96 pKa = 3.6VNASEE101 pKa = 5.64AEE103 pKa = 4.08WLDD106 pKa = 3.61SLLEE110 pKa = 4.03TLGEE114 pKa = 4.43DD115 pKa = 5.08GEE117 pKa = 4.67DD118 pKa = 3.34LEE120 pKa = 4.5QIRR123 pKa = 11.84VSVLPVEE130 pKa = 5.08DD131 pKa = 5.91DD132 pKa = 3.96EE133 pKa = 5.98DD134 pKa = 4.37LQTSPLVSPMSSSDD148 pKa = 3.74DD149 pKa = 3.86LLNQLAYY156 pKa = 9.73YY157 pKa = 9.22SPPIAVPYY165 pKa = 8.01PVPYY169 pKa = 10.28PPFHH173 pKa = 7.16PPLIRR178 pKa = 11.84PCEE181 pKa = 4.17LGPIIDD187 pKa = 4.37SPLLSSLPAYY197 pKa = 9.88DD198 pKa = 5.69DD199 pKa = 4.04PLPYY203 pKa = 10.4YY204 pKa = 9.98DD205 pKa = 4.26TDD207 pKa = 4.23EE208 pKa = 5.73IDD210 pKa = 4.47DD211 pKa = 4.66LSVPDD216 pKa = 5.32AIEE219 pKa = 4.35DD220 pKa = 3.92TSDD223 pKa = 3.9DD224 pKa = 3.83EE225 pKa = 5.31SDD227 pKa = 3.6IPSTPSLVRR236 pKa = 11.84SSTQSFVDD244 pKa = 3.92PASVPLPAEE253 pKa = 3.93RR254 pKa = 11.84RR255 pKa = 11.84RR256 pKa = 11.84QRR258 pKa = 11.84MQPHH262 pKa = 6.15VYY264 pKa = 9.57IGTMDD269 pKa = 3.98PYY271 pKa = 10.73FYY273 pKa = 10.4PFEE276 pKa = 5.39LDD278 pKa = 3.61PLPFPDD284 pKa = 3.52EE285 pKa = 4.62DD286 pKa = 4.16RR287 pKa = 11.84FASYY291 pKa = 11.23NPIYY295 pKa = 10.42SEE297 pKa = 4.38CC298 pKa = 3.85

Molecular weight: 33.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8PVY8|F8PVY8_SERL3 Uncharacterized protein OS=Serpula lacrymans var. lacrymans (strain S7.3) OX=936435 GN=SERLA73DRAFT_106193 PE=4 SV=1
MM1 pKa = 7.52PRR3 pKa = 11.84ILRR6 pKa = 11.84QLVRR10 pKa = 11.84LLSRR14 pKa = 11.84PPTLQALPSRR24 pKa = 11.84AASHH28 pKa = 6.46LRR30 pKa = 11.84AFQATLPFCSQILNNHH46 pKa = 5.78NPLLFGPAQHH56 pKa = 6.97RR57 pKa = 11.84ISSPILAAVHH67 pKa = 4.82QVRR70 pKa = 11.84FAARR74 pKa = 11.84GTEE77 pKa = 4.06YY78 pKa = 10.7QPSQRR83 pKa = 11.84KK84 pKa = 8.85RR85 pKa = 11.84KK86 pKa = 9.17RR87 pKa = 11.84KK88 pKa = 9.56HH89 pKa = 5.55GFLARR94 pKa = 11.84KK95 pKa = 9.69RR96 pKa = 11.84SITGRR101 pKa = 11.84RR102 pKa = 11.84ILARR106 pKa = 11.84RR107 pKa = 11.84KK108 pKa = 9.31ARR110 pKa = 11.84GRR112 pKa = 11.84TFLSHH117 pKa = 6.99

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14339

0

14339

4645570

42

4889

324.0

36.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.881 ± 0.019

1.372 ± 0.008

5.523 ± 0.016

5.816 ± 0.021

3.836 ± 0.013

6.352 ± 0.021

2.674 ± 0.012

5.269 ± 0.015

4.913 ± 0.024

9.298 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.23 ± 0.008

3.766 ± 0.012

5.952 ± 0.024

3.928 ± 0.014

5.825 ± 0.021

8.72 ± 0.026

5.947 ± 0.015

6.367 ± 0.016

1.491 ± 0.008

2.841 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski