Bacteroides sp. CAG:462

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2834 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7CTH7|R7CTH7_9BACE Uncharacterized protein OS=Bacteroides sp. CAG:462 OX=1262740 GN=BN666_01041 PE=4 SV=1
MM1 pKa = 7.3IRR3 pKa = 11.84YY4 pKa = 7.53FIPFTLLCATLAFASCGDD22 pKa = 3.98NEE24 pKa = 5.32DD25 pKa = 3.84NDD27 pKa = 3.54IDD29 pKa = 4.1VPTEE33 pKa = 3.81EE34 pKa = 4.59VVPGTNHH41 pKa = 5.82NVLVAYY47 pKa = 9.88FSEE50 pKa = 4.42PLPDD54 pKa = 4.28GVDD57 pKa = 3.39ASTSASRR64 pKa = 11.84VIVNGDD70 pKa = 3.37LYY72 pKa = 11.59GSVEE76 pKa = 4.13YY77 pKa = 9.27MAMVISEE84 pKa = 4.17ATGADD89 pKa = 3.26VVRR92 pKa = 11.84IQTATPYY99 pKa = 10.24PGNFDD104 pKa = 4.94DD105 pKa = 6.43LASQADD111 pKa = 3.77NEE113 pKa = 4.38RR114 pKa = 11.84QNDD117 pKa = 3.37IRR119 pKa = 11.84PALATEE125 pKa = 4.39IEE127 pKa = 4.23NFDD130 pKa = 4.19DD131 pKa = 4.06YY132 pKa = 12.06DD133 pKa = 4.53VIFVGYY139 pKa = 8.16PIWWYY144 pKa = 9.73QMPMAMYY151 pKa = 10.49SFFDD155 pKa = 3.84EE156 pKa = 4.35YY157 pKa = 11.47DD158 pKa = 3.44FTGKK162 pKa = 8.06TLIPFSSHH170 pKa = 6.45GGSGWSGTVDD180 pKa = 5.13DD181 pKa = 4.73IAGMEE186 pKa = 4.32PNATMVDD193 pKa = 4.23GYY195 pKa = 10.84SISRR199 pKa = 11.84NSVEE203 pKa = 4.16GSADD207 pKa = 4.61GIRR210 pKa = 11.84EE211 pKa = 3.85WLGRR215 pKa = 11.84IGMSEE220 pKa = 3.68QQ221 pKa = 3.09

Molecular weight: 24.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7CT45|R7CT45_9BACE Glycosyl hydrolase family 88 OS=Bacteroides sp. CAG:462 OX=1262740 GN=BN666_00886 PE=4 SV=1
MM1 pKa = 8.13RR2 pKa = 11.84IFLCRR7 pKa = 11.84HH8 pKa = 4.41AHH10 pKa = 5.76LLVTRR15 pKa = 11.84SSSPWYY21 pKa = 9.48RR22 pKa = 11.84FARR25 pKa = 11.84KK26 pKa = 9.72GSGEE30 pKa = 3.88MSISKK35 pKa = 9.04TGVTLIVTGVYY46 pKa = 9.53RR47 pKa = 11.84KK48 pKa = 9.75SYY50 pKa = 10.45RR51 pKa = 11.84GDD53 pKa = 3.41GEE55 pKa = 3.89IGLRR59 pKa = 11.84CIMQGHH65 pKa = 6.65FLLLIARR72 pKa = 11.84ASAISCMSATGNTVEE87 pKa = 4.76TDD89 pKa = 3.25NSICNSLTWKK99 pKa = 9.67PRR101 pKa = 11.84RR102 pKa = 11.84AKK104 pKa = 9.87MRR106 pKa = 11.84FIPVWAGDD114 pKa = 3.77TLSAVCLPITTLRR127 pKa = 11.84CGGSYY132 pKa = 9.17LTTEE136 pKa = 4.54RR137 pKa = 11.84MPSTFCAVRR146 pKa = 11.84MVRR149 pKa = 11.84RR150 pKa = 3.71

Molecular weight: 16.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2834

0

2834

1012360

29

1877

357.2

40.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.776 ± 0.05

1.317 ± 0.018

5.902 ± 0.035

6.414 ± 0.044

4.324 ± 0.028

7.14 ± 0.042

2.105 ± 0.021

5.752 ± 0.042

5.325 ± 0.04

9.486 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.627 ± 0.018

4.467 ± 0.039

4.042 ± 0.027

3.46 ± 0.025

5.78 ± 0.04

5.785 ± 0.04

5.819 ± 0.033

6.835 ± 0.045

1.296 ± 0.02

4.346 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski