Clostridium tetanomorphum DSM 665

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium tetanomorphum

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4354 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A037Z4U8|A0A037Z4U8_CLOTT DegV protein OS=Clostridium tetanomorphum DSM 665 OX=1230342 GN=CTM_12215 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.43SMTSKK7 pKa = 10.63VSYY10 pKa = 11.08LNGLVDD16 pKa = 4.02GLGIDD21 pKa = 4.09AEE23 pKa = 4.47SKK25 pKa = 8.48EE26 pKa = 4.19GKK28 pKa = 8.92VVRR31 pKa = 11.84EE32 pKa = 3.71IVNVLNEE39 pKa = 3.85MAEE42 pKa = 4.23EE43 pKa = 3.93IEE45 pKa = 4.51EE46 pKa = 4.27IKK48 pKa = 10.85DD49 pKa = 3.6SQRR52 pKa = 11.84DD53 pKa = 3.43IQDD56 pKa = 3.36YY57 pKa = 11.31VDD59 pKa = 5.59ALDD62 pKa = 4.33EE63 pKa = 4.94DD64 pKa = 4.54LTSLEE69 pKa = 4.56DD70 pKa = 4.17DD71 pKa = 5.61LYY73 pKa = 11.51DD74 pKa = 4.55EE75 pKa = 5.68DD76 pKa = 5.66DD77 pKa = 4.36NYY79 pKa = 11.71DD80 pKa = 3.51EE81 pKa = 5.58EE82 pKa = 4.7EE83 pKa = 4.6IEE85 pKa = 5.01DD86 pKa = 4.31FVDD89 pKa = 4.05MTCDD93 pKa = 3.12NCGEE97 pKa = 4.25TVYY100 pKa = 10.61IDD102 pKa = 3.38TAILQGKK109 pKa = 9.36DD110 pKa = 3.36KK111 pKa = 9.86ITCPNCHH118 pKa = 6.61KK119 pKa = 10.31EE120 pKa = 4.18IKK122 pKa = 10.29LHH124 pKa = 6.27LNCDD128 pKa = 3.93CEE130 pKa = 4.3EE131 pKa = 4.43CDD133 pKa = 4.03

Molecular weight: 15.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A037Z3G7|A0A037Z3G7_CLOTT Transposase IS204/IS1001/IS1096/IS1165 OS=Clostridium tetanomorphum DSM 665 OX=1230342 GN=CTM_19599 PE=4 SV=1
MM1 pKa = 7.2SRR3 pKa = 11.84EE4 pKa = 4.13AGRR7 pKa = 11.84EE8 pKa = 4.06SNGRR12 pKa = 11.84RR13 pKa = 11.84GGGRR17 pKa = 11.84MRR19 pKa = 11.84RR20 pKa = 11.84AKK22 pKa = 10.52KK23 pKa = 9.81KK24 pKa = 9.12VCSFCVDD31 pKa = 3.03KK32 pKa = 11.54AEE34 pKa = 4.75SIDD37 pKa = 3.62YY38 pKa = 10.94KK39 pKa = 11.04DD40 pKa = 3.2INKK43 pKa = 9.01LRR45 pKa = 11.84KK46 pKa = 9.78YY47 pKa = 8.01ITEE50 pKa = 4.01RR51 pKa = 11.84GKK53 pKa = 10.04ILPRR57 pKa = 11.84RR58 pKa = 11.84ISGNCAKK65 pKa = 10.19HH66 pKa = 5.27QRR68 pKa = 11.84EE69 pKa = 4.01LTEE72 pKa = 4.53AIKK75 pKa = 10.4RR76 pKa = 11.84ARR78 pKa = 11.84NIALLPFTTEE88 pKa = 3.59

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4354

0

4354

1299553

22

2884

298.5

33.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.38 ± 0.036

1.212 ± 0.015

5.241 ± 0.031

7.358 ± 0.044

4.425 ± 0.031

6.28 ± 0.035

1.352 ± 0.016

10.524 ± 0.052

9.578 ± 0.04

8.974 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.017

6.626 ± 0.04

2.773 ± 0.023

2.312 ± 0.016

3.265 ± 0.027

6.226 ± 0.029

4.737 ± 0.029

6.231 ± 0.036

0.698 ± 0.011

4.17 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski