Faeces associated gemycircularvirus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus furse1; Fur seal associated gemycircularvirus 1

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1YSG3|T1YSG3_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 4 OX=1391033 PE=3 SV=1
MM1 pKa = 7.8PPKK4 pKa = 10.25QFQFQARR11 pKa = 11.84YY12 pKa = 10.09VLLTYY17 pKa = 9.16AQCGDD22 pKa = 3.48LDD24 pKa = 3.38AWAVNDD30 pKa = 4.01HH31 pKa = 6.39LAFLGGEE38 pKa = 4.2CIIGRR43 pKa = 11.84EE44 pKa = 3.96HH45 pKa = 6.62HH46 pKa = 7.07ADD48 pKa = 3.5GGTHH52 pKa = 6.57LHH54 pKa = 6.66AFCDD58 pKa = 5.35FSRR61 pKa = 11.84KK62 pKa = 8.68FRR64 pKa = 11.84SRR66 pKa = 11.84RR67 pKa = 11.84PDD69 pKa = 3.12VFDD72 pKa = 3.47VGGFHH77 pKa = 7.33PNIEE81 pKa = 4.18ASRR84 pKa = 11.84GRR86 pKa = 11.84PEE88 pKa = 4.03GGYY91 pKa = 10.62DD92 pKa = 3.46YY93 pKa = 10.75AIKK96 pKa = 10.94DD97 pKa = 3.59GDD99 pKa = 4.03VVAGGLSRR107 pKa = 11.84PGGRR111 pKa = 11.84GVYY114 pKa = 9.87EE115 pKa = 4.95DD116 pKa = 3.62VSSWSIIVSQEE127 pKa = 3.87SEE129 pKa = 3.96GDD131 pKa = 3.02FWEE134 pKa = 5.0CVARR138 pKa = 11.84LDD140 pKa = 5.07PRR142 pKa = 11.84SLCTNYY148 pKa = 10.59NSLRR152 pKa = 11.84AYY154 pKa = 10.4ANWKK158 pKa = 8.87YY159 pKa = 10.76RR160 pKa = 11.84PTPVQYY166 pKa = 9.84VHH168 pKa = 6.85PAGVEE173 pKa = 4.11FEE175 pKa = 4.61LGMVPEE181 pKa = 4.27LAVWRR186 pKa = 11.84EE187 pKa = 3.5IALGANRR194 pKa = 11.84QRR196 pKa = 11.84GILVIYY202 pKa = 10.42GDD204 pKa = 3.62TRR206 pKa = 11.84LGKK209 pKa = 7.53TLWARR214 pKa = 11.84SLGPHH219 pKa = 7.34IYY221 pKa = 10.16TIGQMSGEE229 pKa = 4.38VILRR233 pKa = 11.84DD234 pKa = 4.51GPDD237 pKa = 2.84ADD239 pKa = 3.79YY240 pKa = 11.53AVFDD244 pKa = 4.52DD245 pKa = 4.37MRR247 pKa = 11.84GGLDD251 pKa = 4.4FFHH254 pKa = 7.02GWKK257 pKa = 9.72EE258 pKa = 3.74WFGCQTVVTVKK269 pKa = 10.62KK270 pKa = 10.31LYY272 pKa = 9.75KK273 pKa = 10.5DD274 pKa = 3.56PMQMPWGKK282 pKa = 9.35PVIWLANRR290 pKa = 11.84DD291 pKa = 3.65PRR293 pKa = 11.84EE294 pKa = 3.97EE295 pKa = 3.99LRR297 pKa = 11.84DD298 pKa = 3.96SITCHH303 pKa = 6.11TSLGKK308 pKa = 9.69QAAIEE313 pKa = 4.36GDD315 pKa = 3.96IKK317 pKa = 10.76WLDD320 pKa = 3.56GNCIFVEE327 pKa = 4.06LDD329 pKa = 3.15HH330 pKa = 7.64AIFRR334 pKa = 11.84ANTEE338 pKa = 3.84

Molecular weight: 38.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1YSG3|T1YSG3_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 4 OX=1391033 PE=3 SV=1
MM1 pKa = 7.58AARR4 pKa = 11.84RR5 pKa = 11.84LPRR8 pKa = 11.84SLPTGRR14 pKa = 11.84YY15 pKa = 7.85GRR17 pKa = 11.84AGRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84AARR25 pKa = 11.84YY26 pKa = 5.87PRR28 pKa = 11.84KK29 pKa = 9.67RR30 pKa = 11.84RR31 pKa = 11.84STGRR35 pKa = 11.84KK36 pKa = 5.27RR37 pKa = 11.84TYY39 pKa = 10.36RR40 pKa = 11.84KK41 pKa = 8.39RR42 pKa = 11.84TMTRR46 pKa = 11.84RR47 pKa = 11.84SILNVTSEE55 pKa = 4.04KK56 pKa = 10.67KK57 pKa = 9.45RR58 pKa = 11.84DD59 pKa = 3.41KK60 pKa = 10.34MLQITNSTAVSQSGSGTYY78 pKa = 10.62LSQSRR83 pKa = 11.84NYY85 pKa = 9.54YY86 pKa = 9.43RR87 pKa = 11.84RR88 pKa = 11.84LVYY91 pKa = 9.94VAAFVWCATARR102 pKa = 11.84DD103 pKa = 3.99NTRR106 pKa = 11.84SSTATQPGNKK116 pKa = 9.05FDD118 pKa = 4.82DD119 pKa = 4.04STRR122 pKa = 11.84TASSCYY128 pKa = 8.78MVGLKK133 pKa = 8.27EE134 pKa = 4.32TIEE137 pKa = 4.06LQCPDD142 pKa = 3.65GLPWQWRR149 pKa = 11.84RR150 pKa = 11.84ICVTMKK156 pKa = 9.98GTGTFAGTLNTGTSTFYY173 pKa = 10.34TALEE177 pKa = 4.14TSNGFSRR184 pKa = 11.84VLNQILAADD193 pKa = 4.04RR194 pKa = 11.84LSFYY198 pKa = 9.92GTLFQGQQDD207 pKa = 4.15VDD209 pKa = 3.3WADD212 pKa = 3.22PMTAKK217 pKa = 10.21VDD219 pKa = 3.71NEE221 pKa = 4.16RR222 pKa = 11.84LTVKK226 pKa = 10.22YY227 pKa = 10.77DD228 pKa = 3.32KK229 pKa = 10.76TITLASGNDD238 pKa = 3.21DD239 pKa = 3.46GMIRR243 pKa = 11.84KK244 pKa = 9.21YY245 pKa = 10.92SRR247 pKa = 11.84YY248 pKa = 9.65HH249 pKa = 7.06RR250 pKa = 11.84MGHH253 pKa = 4.96NLEE256 pKa = 4.42YY257 pKa = 10.98NDD259 pKa = 5.19DD260 pKa = 3.83EE261 pKa = 5.47SGGTKK266 pKa = 9.19TAGQFSVTSKK276 pKa = 10.94KK277 pKa = 11.02GMGDD281 pKa = 3.1YY282 pKa = 9.96WVIDD286 pKa = 3.58LFRR289 pKa = 11.84PRR291 pKa = 11.84VGSTASNQMLFGTEE305 pKa = 3.75STLYY309 pKa = 7.82WHH311 pKa = 6.78EE312 pKa = 4.04RR313 pKa = 3.41

Molecular weight: 35.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

651

313

338

325.5

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.066 ± 0.239

1.843 ± 0.379

6.605 ± 0.788

4.301 ± 0.957

3.994 ± 0.536

9.524 ± 0.817

2.151 ± 0.8

3.994 ± 0.965

4.301 ± 0.544

7.066 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.458 ± 0.495

3.379 ± 0.519

3.84 ± 0.862

3.533 ± 0.202

9.37 ± 1.431

6.452 ± 1.46

7.373 ± 2.771

5.684 ± 0.813

2.611 ± 0.466

4.455 ± 0.441
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski