Barley yellow dwarf virus (isolate MAV-PS1) (BYDV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus; Barley yellow dwarf virus MAV

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q65873|Q65873_BYDV1 ORF6 OS=Barley yellow dwarf virus (isolate MAV-PS1) OX=31723 PE=4 SV=1
VV1 pKa = 7.52DD2 pKa = 3.85SSTPEE7 pKa = 4.32PSPQPQPEE15 pKa = 4.54PKK17 pKa = 9.45PDD19 pKa = 3.89PQPTPEE25 pKa = 4.01PRR27 pKa = 11.84QKK29 pKa = 10.82RR30 pKa = 11.84FFEE33 pKa = 4.55YY34 pKa = 10.2VGTPYY39 pKa = 10.92VVIQTRR45 pKa = 11.84EE46 pKa = 3.99SSDD49 pKa = 3.82SIAVKK54 pKa = 10.7AMNDD58 pKa = 3.06QSFQYY63 pKa = 10.28IEE65 pKa = 4.51NEE67 pKa = 3.83TSEE70 pKa = 4.0QRR72 pKa = 11.84TVKK75 pKa = 10.49AWWNSNNSVQAQAAFIFPIPAGEE98 pKa = 3.91YY99 pKa = 9.93SVNISCEE106 pKa = 3.87GLQSVDD112 pKa = 3.65HH113 pKa = 6.79IGGNRR118 pKa = 11.84DD119 pKa = 3.66GYY121 pKa = 10.15WIGLIAYY128 pKa = 7.77QSQSGDD134 pKa = 3.12YY135 pKa = 8.92WGVGNYY141 pKa = 9.0VGCDD145 pKa = 2.86ITNLLGTNTWRR156 pKa = 11.84PGHH159 pKa = 6.74EE160 pKa = 3.96DD161 pKa = 4.64LEE163 pKa = 4.69LNSCKK168 pKa = 9.77FTDD171 pKa = 4.14GQIVEE176 pKa = 4.29RR177 pKa = 11.84DD178 pKa = 3.46AVISFHH184 pKa = 5.98VKK186 pKa = 10.21ARR188 pKa = 11.84GADD191 pKa = 3.29PKK193 pKa = 10.51FYY195 pKa = 11.18LMAPKK200 pKa = 9.75TMKK203 pKa = 10.13ADD205 pKa = 3.08KK206 pKa = 9.79YY207 pKa = 10.38NYY209 pKa = 7.68VVSYY213 pKa = 10.89GGYY216 pKa = 6.85TDD218 pKa = 3.8KK219 pKa = 11.13RR220 pKa = 11.84MEE222 pKa = 4.54FGTISVTVDD231 pKa = 3.07EE232 pKa = 5.17SDD234 pKa = 3.85VEE236 pKa = 4.04AEE238 pKa = 3.98RR239 pKa = 11.84YY240 pKa = 9.18SRR242 pKa = 11.84HH243 pKa = 5.04TSTVRR248 pKa = 11.84RR249 pKa = 11.84TEE251 pKa = 3.7NRR253 pKa = 11.84DD254 pKa = 3.64YY255 pKa = 11.54GWMNVLPPYY264 pKa = 10.8NPDD267 pKa = 3.07QVPEE271 pKa = 4.14QEE273 pKa = 4.76DD274 pKa = 3.79EE275 pKa = 4.27QPVVDD280 pKa = 4.82KK281 pKa = 11.25EE282 pKa = 3.98MDD284 pKa = 3.54AGSPIDD290 pKa = 3.9TASLTSDD297 pKa = 3.68TEE299 pKa = 4.03AEE301 pKa = 4.06KK302 pKa = 11.13AFDD305 pKa = 3.97LKK307 pKa = 11.2EE308 pKa = 4.16EE309 pKa = 4.11EE310 pKa = 4.09LTRR313 pKa = 11.84AILEE317 pKa = 4.26YY318 pKa = 10.43EE319 pKa = 4.18AATVSIPDD327 pKa = 3.99AAPDD331 pKa = 3.52ILPSKK336 pKa = 10.88SEE338 pKa = 4.0MSSKK342 pKa = 10.68PIDD345 pKa = 3.54RR346 pKa = 11.84DD347 pKa = 3.49GRR349 pKa = 11.84SLPKK353 pKa = 10.44SQTKK357 pKa = 9.47EE358 pKa = 3.7VLGTYY363 pKa = 9.39QGQNITSDD371 pKa = 3.62DD372 pKa = 4.18VPPVIAEE379 pKa = 4.08KK380 pKa = 10.58LRR382 pKa = 11.84EE383 pKa = 4.08VNRR386 pKa = 11.84APSTLLYY393 pKa = 10.5DD394 pKa = 4.33RR395 pKa = 11.84QPKK398 pKa = 8.56QPKK401 pKa = 9.36NPLTRR406 pKa = 11.84FVLSNKK412 pKa = 9.13TSTASPGSQSSTAGMTRR429 pKa = 11.84EE430 pKa = 3.89QASEE434 pKa = 3.75YY435 pKa = 9.62TRR437 pKa = 11.84IRR439 pKa = 11.84KK440 pKa = 9.16SLGLTAAKK448 pKa = 9.89QYY450 pKa = 10.54KK451 pKa = 10.32ASLDD455 pKa = 3.68DD456 pKa = 3.54TT457 pKa = 4.51

Molecular weight: 50.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65871|Q65871_BYDV1 RNA-directed RNA polymerase (Fragment) OS=Barley yellow dwarf virus (isolate MAV-PS1) OX=31723 PE=4 SV=1
MM1 pKa = 7.28NSVGRR6 pKa = 11.84RR7 pKa = 11.84NNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84NGPRR16 pKa = 11.84RR17 pKa = 11.84ARR19 pKa = 11.84RR20 pKa = 11.84VSAVRR25 pKa = 11.84RR26 pKa = 11.84MVVVQPNRR34 pKa = 11.84AGPKK38 pKa = 8.16RR39 pKa = 11.84RR40 pKa = 11.84ARR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84TRR46 pKa = 11.84GGGANLISGPAGRR59 pKa = 11.84TEE61 pKa = 3.81VFVFSVNDD69 pKa = 3.76LKK71 pKa = 11.54ANSSGTIKK79 pKa = 10.68FGPDD83 pKa = 3.19LSQCPALSGGILKK96 pKa = 10.33SYY98 pKa = 10.55HH99 pKa = 6.6LYY101 pKa = 10.64KK102 pKa = 9.46ITNVKK107 pKa = 10.26VEE109 pKa = 4.92FKK111 pKa = 10.37SHH113 pKa = 6.16ASASTVGAMFIEE125 pKa = 5.12LDD127 pKa = 3.67TWCSQSTLGSYY138 pKa = 10.37INSFTISKK146 pKa = 10.2SATKK150 pKa = 10.09TFTAQQIDD158 pKa = 3.42GKK160 pKa = 7.87EE161 pKa = 4.04FRR163 pKa = 11.84EE164 pKa = 4.2STVNQFYY171 pKa = 10.29MLYY174 pKa = 9.29KK175 pKa = 10.81ANGSTSDD182 pKa = 3.18TAGQFIITIRR192 pKa = 11.84VANMTPKK199 pKa = 10.68

Molecular weight: 21.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

2591

43

867

370.1

41.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.294 ± 0.458

1.621 ± 0.223

5.326 ± 0.541

7.102 ± 0.881

4.091 ± 0.396

5.712 ± 0.434

2.084 ± 0.354

5.403 ± 0.457

7.294 ± 0.816

7.487 ± 0.565

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.279

4.207 ± 0.363

4.323 ± 0.832

4.245 ± 0.77

5.866 ± 0.585

7.951 ± 0.605

5.712 ± 0.609

6.677 ± 0.323

1.003 ± 0.124

3.937 ± 0.406
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski