Pseudomonas guangdongensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2871 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2F7R8|A0A1H2F7R8_9PSED Putative hemolysin OS=Pseudomonas guangdongensis OX=1245526 GN=SAMN05216580_1068 PE=3 SV=1
MM1 pKa = 7.8EE2 pKa = 5.65DD3 pKa = 3.18VIDD6 pKa = 4.77RR7 pKa = 11.84LRR9 pKa = 11.84EE10 pKa = 3.89LNEE13 pKa = 3.83PVPVPLEE20 pKa = 4.35LPDD23 pKa = 4.0EE24 pKa = 4.68DD25 pKa = 5.05LLVEE29 pKa = 4.06IQEE32 pKa = 4.4QILIHH37 pKa = 6.55LPFEE41 pKa = 4.06LRR43 pKa = 11.84EE44 pKa = 4.09YY45 pKa = 10.64LLKK48 pKa = 11.03ASDD51 pKa = 3.6VVYY54 pKa = 10.58GHH56 pKa = 7.07LEE58 pKa = 3.96PVTVCDD64 pKa = 4.4PQSHH68 pKa = 6.48TYY70 pKa = 10.43LPDD73 pKa = 2.96VAARR77 pKa = 11.84AWDD80 pKa = 3.76AGVPRR85 pKa = 11.84YY86 pKa = 8.91LVPLCEE92 pKa = 4.94DD93 pKa = 3.61GGRR96 pKa = 11.84YY97 pKa = 9.29YY98 pKa = 10.79CVAEE102 pKa = 4.66DD103 pKa = 4.67GEE105 pKa = 4.55VLLWEE110 pKa = 5.1DD111 pKa = 4.4GEE113 pKa = 4.33LDD115 pKa = 5.32EE116 pKa = 5.64EE117 pKa = 4.88GTWDD121 pKa = 4.61SLWSWVEE128 pKa = 4.08DD129 pKa = 3.3VWLADD134 pKa = 3.41AA135 pKa = 5.68

Molecular weight: 15.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2FAL7|A0A1H2FAL7_9PSED Shikimate dehydrogenase (NADP(+)) OS=Pseudomonas guangdongensis OX=1245526 GN=aroE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.02RR12 pKa = 11.84ARR14 pKa = 11.84NHH16 pKa = 5.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 9.11RR41 pKa = 11.84LTTVV45 pKa = 2.62

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2871

0

2871

945301

29

2302

329.3

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.65 ± 0.077

1.076 ± 0.014

5.262 ± 0.033

6.148 ± 0.04

3.352 ± 0.028

8.271 ± 0.042

2.299 ± 0.024

4.233 ± 0.034

2.798 ± 0.038

12.736 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.055 ± 0.022

2.461 ± 0.032

4.987 ± 0.033

4.434 ± 0.036

7.597 ± 0.053

5.102 ± 0.033

4.053 ± 0.035

6.798 ± 0.036

1.407 ± 0.022

2.281 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski