Dictyostelium purpureum (Slime mold)

Taxonomy: cellular organisms; Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales; Dictyosteliaceae; Dictyostelium

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0Z754|F0Z754_DICPU Expressed protein (Fragment) OS=Dictyostelium purpureum OX=5786 GN=DICPUDRAFT_71378 PE=4 SV=1
MM1 pKa = 7.45NGAKK5 pKa = 10.49VNTTFINLFLKK16 pKa = 10.59EE17 pKa = 4.02FLIGLNRR24 pKa = 11.84VDD26 pKa = 5.18LKK28 pKa = 11.5VIFCGCSSGGFNTEE42 pKa = 3.78NGSEE46 pKa = 4.22GEE48 pKa = 4.15NNEE51 pKa = 4.1EE52 pKa = 4.77EE53 pKa = 4.26EE54 pKa = 5.93DD55 pKa = 5.0GDD57 pKa = 4.87DD58 pKa = 4.63DD59 pKa = 5.07HH60 pKa = 9.1NGYY63 pKa = 10.33DD64 pKa = 4.98DD65 pKa = 5.51HH66 pKa = 8.85DD67 pKa = 5.74HH68 pKa = 7.91DD69 pKa = 5.78DD70 pKa = 4.26ADD72 pKa = 5.38DD73 pKa = 4.56GGDD76 pKa = 3.55YY77 pKa = 10.9DD78 pKa = 6.32AEE80 pKa = 6.21DD81 pKa = 5.0GDD83 pKa = 5.78HH84 pKa = 7.79DD85 pKa = 4.96DD86 pKa = 5.3ADD88 pKa = 4.61DD89 pKa = 4.68GGDD92 pKa = 3.78YY93 pKa = 11.14DD94 pKa = 5.84SDD96 pKa = 4.57DD97 pKa = 5.1DD98 pKa = 5.65GDD100 pKa = 5.43HH101 pKa = 7.24EE102 pKa = 5.46DD103 pKa = 4.17HH104 pKa = 7.84DD105 pKa = 4.9DD106 pKa = 5.31DD107 pKa = 5.43EE108 pKa = 5.42RR109 pKa = 11.84ADD111 pKa = 3.99YY112 pKa = 11.15DD113 pKa = 5.15DD114 pKa = 4.51EE115 pKa = 5.75RR116 pKa = 11.84ADD118 pKa = 3.96HH119 pKa = 7.28DD120 pKa = 4.93HH121 pKa = 7.99DD122 pKa = 5.89DD123 pKa = 4.04DD124 pKa = 4.57HH125 pKa = 8.59KK126 pKa = 11.31GAYY129 pKa = 9.16FCWLYY134 pKa = 10.33STTIYY139 pKa = 10.75FSTTDD144 pKa = 3.86GIKK147 pKa = 10.16RR148 pKa = 11.84VITIIYY154 pKa = 9.47RR155 pKa = 11.84IYY157 pKa = 10.98

Molecular weight: 17.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0ZWX1|F0ZWX1_DICPU Uncharacterized protein (Fragment) OS=Dictyostelium purpureum OX=5786 GN=DICPUDRAFT_156568 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.68GNGKK6 pKa = 7.37PTTVIKK12 pKa = 10.59LQAKK16 pKa = 8.47PAGAAPPPHH25 pKa = 6.79SAQPPQPPQARR36 pKa = 11.84PVSQIQNRR44 pKa = 11.84PPPSTKK50 pKa = 9.2WQFTIAGGQPHH61 pKa = 7.51PPMNRR66 pKa = 11.84PPPPVAGGQPHH77 pKa = 7.27PPMNRR82 pKa = 11.84PPPPGQPPMNRR93 pKa = 11.84PPPPGQPPMNRR104 pKa = 11.84PPPPGQPRR112 pKa = 11.84PPPPGQPNMPPRR124 pKa = 11.84PVSQMNGGAPTVGGGVGGPPPKK146 pKa = 9.41PQRR149 pKa = 11.84PGVPPPPGQPNMPPRR164 pKa = 11.84PMTQPPNGAAPPPKK178 pKa = 9.22PQRR181 pKa = 11.84PGAGPPLPPRR191 pKa = 4.11

Molecular weight: 19.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12347

0

12347

5964926

49

9355

483.1

55.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.447 ± 0.02

1.471 ± 0.012

5.289 ± 0.016

6.214 ± 0.025

4.94 ± 0.017

4.403 ± 0.022

1.88 ± 0.011

8.317 ± 0.022

7.928 ± 0.024

8.998 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.608 ± 0.008

9.333 ± 0.042

4.181 ± 0.022

4.512 ± 0.026

3.0 ± 0.015

9.462 ± 0.032

5.664 ± 0.021

4.702 ± 0.018

0.753 ± 0.006

3.901 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski