Oceanospirillum linum

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3358 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1T1HDY8|A0A1T1HDY8_OCELI Intracellular proteinase I OS=Oceanospirillum linum OX=966 GN=BTA35_0200210 PE=4 SV=1
MM1 pKa = 7.44NPFPIFIDD9 pKa = 4.37FEE11 pKa = 4.54CSSPDD16 pKa = 3.27EE17 pKa = 4.94DD18 pKa = 3.92GFPIAVCWSTPDD30 pKa = 3.62GQVKK34 pKa = 8.56STLITPEE41 pKa = 4.59DD42 pKa = 3.7DD43 pKa = 3.29WLDD46 pKa = 3.69SHH48 pKa = 6.91SHH50 pKa = 5.21QFDD53 pKa = 3.64DD54 pKa = 4.67AAIDD58 pKa = 3.59IDD60 pKa = 4.8DD61 pKa = 4.54LMLHH65 pKa = 6.25GVSALEE71 pKa = 4.73VIRR74 pKa = 11.84EE75 pKa = 4.08LQGDD79 pKa = 3.67IDD81 pKa = 4.65SDD83 pKa = 3.46IVYY86 pKa = 10.43VDD88 pKa = 3.58GSGEE92 pKa = 4.05DD93 pKa = 3.8EE94 pKa = 4.55HH95 pKa = 7.93WLDD98 pKa = 4.08MLFDD102 pKa = 4.57SYY104 pKa = 11.9QMNVQFAFEE113 pKa = 4.24PAASLYY119 pKa = 10.68EE120 pKa = 3.75IGFEE124 pKa = 3.68HH125 pKa = 6.27WQDD128 pKa = 4.49LRR130 pKa = 11.84SEE132 pKa = 4.04WMAEE136 pKa = 3.67RR137 pKa = 11.84GMDD140 pKa = 4.06PYY142 pKa = 11.06QSEE145 pKa = 4.64ANVLSMLHH153 pKa = 5.01VHH155 pKa = 6.26QALTEE160 pKa = 4.3GGVV163 pKa = 3.06

Molecular weight:
18.48 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1T1HCD3|A0A1T1HCD3_OCELI Acyl-CoA dehydrogenase OS=Oceanospirillum linum OX=966 GN=BTA35_0205625 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.14NGRR28 pKa = 11.84KK29 pKa = 9.28IITRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.54GRR39 pKa = 11.84VRR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3358

0

3358

1056198

29

3587

314.5

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.127 ± 0.051

0.972 ± 0.014

5.667 ± 0.048

6.543 ± 0.043

3.846 ± 0.028

7.113 ± 0.037

2.265 ± 0.022

5.838 ± 0.03

4.671 ± 0.039

10.918 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.023

3.6 ± 0.026

4.228 ± 0.03

4.896 ± 0.036

5.309 ± 0.036

6.482 ± 0.038

5.105 ± 0.035

6.799 ± 0.036

1.229 ± 0.017

2.757 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski