Dragonfly cyclicusvirus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0A2I6|K0A2I6_9VIRU ATP-dependent helicase Rep OS=Dragonfly cyclicusvirus OX=1234878 PE=3 SV=1
MM1 pKa = 7.7GSPAIGWCFTLNNYY15 pKa = 7.64TEE17 pKa = 4.27IDD19 pKa = 3.89EE20 pKa = 4.59QVLQALDD27 pKa = 3.55VKK29 pKa = 10.38YY30 pKa = 10.31LVYY33 pKa = 10.36GHH35 pKa = 6.25EE36 pKa = 4.31VGEE39 pKa = 4.65GGTPHH44 pKa = 6.18LQGYY48 pKa = 9.8LEE50 pKa = 4.01LHH52 pKa = 5.49KK53 pKa = 10.61KK54 pKa = 10.17KK55 pKa = 10.44RR56 pKa = 11.84FKK58 pKa = 10.21QVKK61 pKa = 8.27EE62 pKa = 3.93MLGEE66 pKa = 4.41RR67 pKa = 11.84YY68 pKa = 9.47HH69 pKa = 8.44IEE71 pKa = 3.81MRR73 pKa = 11.84RR74 pKa = 11.84GTAAEE79 pKa = 3.62VDD81 pKa = 3.9AYY83 pKa = 10.84CRR85 pKa = 11.84KK86 pKa = 9.61QDD88 pKa = 3.65EE89 pKa = 4.77NNVFTKK95 pKa = 10.12GTMGGQGLRR104 pKa = 11.84SDD106 pKa = 4.37LDD108 pKa = 3.62RR109 pKa = 11.84VRR111 pKa = 11.84LLAAEE116 pKa = 4.13SGMRR120 pKa = 11.84AVAATANYY128 pKa = 9.09QQIKK132 pKa = 7.05TAEE135 pKa = 3.81IYY137 pKa = 9.05LTYY140 pKa = 10.69NEE142 pKa = 5.17EE143 pKa = 3.93PRR145 pKa = 11.84SWKK148 pKa = 8.39PTVYY152 pKa = 9.93WIYY155 pKa = 10.57GASGTGKK162 pKa = 7.55TRR164 pKa = 11.84KK165 pKa = 9.74AYY167 pKa = 8.12EE168 pKa = 3.66ICGTEE173 pKa = 3.74DD174 pKa = 3.55TYY176 pKa = 12.08VKK178 pKa = 10.75VGTHH182 pKa = 5.73KK183 pKa = 10.04WWDD186 pKa = 3.83GYY188 pKa = 11.06DD189 pKa = 3.18GHH191 pKa = 6.78EE192 pKa = 4.33NVIIDD197 pKa = 4.31DD198 pKa = 4.33FRR200 pKa = 11.84PMGIPFVDD208 pKa = 3.9LLGILDD214 pKa = 3.66RR215 pKa = 11.84YY216 pKa = 7.81EE217 pKa = 3.87FRR219 pKa = 11.84VEE221 pKa = 4.06VKK223 pKa = 10.7GKK225 pKa = 8.16MRR227 pKa = 11.84QMLAKK232 pKa = 9.13TIVITTIYY240 pKa = 9.09PPEE243 pKa = 3.92VLYY246 pKa = 11.46VNLKK250 pKa = 10.53DD251 pKa = 4.85EE252 pKa = 4.95PLQQLLRR259 pKa = 11.84RR260 pKa = 11.84IDD262 pKa = 3.35HH263 pKa = 7.12FINFNLNSILDD274 pKa = 4.31NIEE277 pKa = 4.49HH278 pKa = 6.31EE279 pKa = 4.48FEE281 pKa = 5.02DD282 pKa = 4.58AEE284 pKa = 4.26EE285 pKa = 5.46DD286 pKa = 3.71INSS289 pKa = 3.43

Molecular weight: 33.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0A2I6|K0A2I6_9VIRU ATP-dependent helicase Rep OS=Dragonfly cyclicusvirus OX=1234878 PE=3 SV=1
MM1 pKa = 7.63PKK3 pKa = 8.88KK4 pKa = 9.41TSTRR8 pKa = 11.84KK9 pKa = 9.37NGRR12 pKa = 11.84RR13 pKa = 11.84TAKK16 pKa = 9.91RR17 pKa = 11.84RR18 pKa = 11.84PTVKK22 pKa = 9.57RR23 pKa = 11.84VMRR26 pKa = 11.84MVKK29 pKa = 8.79QLKK32 pKa = 10.2PEE34 pKa = 4.14VKK36 pKa = 10.18EE37 pKa = 3.31ITYY40 pKa = 10.76AFNALPTASLASVSFGSGAAMSTLASGITQGTGRR74 pKa = 11.84SQRR77 pKa = 11.84VGNMVRR83 pKa = 11.84VIGIQIRR90 pKa = 11.84LAISGADD97 pKa = 3.16STNNFRR103 pKa = 11.84IMLVKK108 pKa = 10.35PKK110 pKa = 10.37SSPFNTGSALIQNILSGTPSGTTQWLAPIDD140 pKa = 3.51TDD142 pKa = 3.68RR143 pKa = 11.84YY144 pKa = 10.05HH145 pKa = 8.86VLFDD149 pKa = 3.15KK150 pKa = 10.74MKK152 pKa = 10.3FFRR155 pKa = 11.84TMPLDD160 pKa = 4.13GNSSSTTLPVWIFKK174 pKa = 10.69KK175 pKa = 10.5FIKK178 pKa = 9.63TRR180 pKa = 11.84STIQWDD186 pKa = 3.77AEE188 pKa = 4.28NNPDD192 pKa = 3.51PRR194 pKa = 11.84DD195 pKa = 3.57WILAAISDD203 pKa = 4.49SNLVPNPGAIAGHH216 pKa = 5.47VKK218 pKa = 10.5LYY220 pKa = 9.4YY221 pKa = 10.2TDD223 pKa = 3.38AA224 pKa = 5.84

Molecular weight: 24.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

513

224

289

256.5

29.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.628 ± 0.949

0.585 ± 0.394

5.458 ± 0.67

5.653 ± 2.906

3.509 ± 0.343

7.602 ± 0.61

1.949 ± 0.712

6.823 ± 0.216

6.628 ± 0.347

7.602 ± 0.61

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.119 ± 0.305

4.873 ± 0.326

4.483 ± 1.19

3.509 ± 0.259

6.433 ± 0.478

5.458 ± 2.639

7.602 ± 1.194

6.238 ± 0.293

1.754 ± 0.021

4.094 ± 1.555
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski