Pseudomonas mangrovi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3682 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T5PAP5|A0A2T5PAP5_9PSED Uncharacterized protein OS=Pseudomonas mangrovi OX=2161748 GN=DBO85_07610 PE=4 SV=1
MM1 pKa = 7.27QPLTADD7 pKa = 3.58DD8 pKa = 4.83AAWQAACDD16 pKa = 4.02ANGVDD21 pKa = 3.71PQQRR25 pKa = 11.84LQARR29 pKa = 11.84RR30 pKa = 11.84EE31 pKa = 4.15VLEE34 pKa = 4.54HH35 pKa = 7.25ADD37 pKa = 4.88SLDD40 pKa = 3.64CCLYY44 pKa = 10.87RR45 pKa = 11.84PDD47 pKa = 5.04EE48 pKa = 5.05DD49 pKa = 5.07DD50 pKa = 4.94ADD52 pKa = 4.41AEE54 pKa = 4.19EE55 pKa = 5.45LDD57 pKa = 4.36LGDD60 pKa = 5.55ARR62 pKa = 11.84VLLQGPFQPPADD74 pKa = 3.84WDD76 pKa = 3.77EE77 pKa = 4.38QTCEE81 pKa = 4.36DD82 pKa = 4.61YY83 pKa = 11.28FDD85 pKa = 5.06GSDD88 pKa = 3.39PADD91 pKa = 3.59FVAARR96 pKa = 11.84LEE98 pKa = 4.18PCAAPGSRR106 pKa = 11.84QHH108 pKa = 6.47FRR110 pKa = 11.84PQPGDD115 pKa = 3.38YY116 pKa = 10.51VAVSEE121 pKa = 4.28ADD123 pKa = 3.21GRR125 pKa = 11.84VQMYY129 pKa = 10.0YY130 pKa = 10.51LYY132 pKa = 10.71EE133 pKa = 4.48CLEE136 pKa = 3.95QDD138 pKa = 4.95DD139 pKa = 5.15GLACVLIRR147 pKa = 11.84EE148 pKa = 4.3HH149 pKa = 7.83DD150 pKa = 3.72EE151 pKa = 3.98LL152 pKa = 6.24

Molecular weight: 16.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T5P9Z7|A0A2T5P9Z7_9PSED Uncharacterized protein OS=Pseudomonas mangrovi OX=2161748 GN=DBO85_10850 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3682

0

3682

1184299

25

2464

321.6

35.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.369 ± 0.045

1.062 ± 0.015

5.401 ± 0.032

6.24 ± 0.044

3.517 ± 0.025

8.028 ± 0.053

2.215 ± 0.022

4.491 ± 0.03

2.885 ± 0.035

12.451 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.019

2.661 ± 0.029

4.878 ± 0.028

4.685 ± 0.032

7.348 ± 0.052

5.726 ± 0.035

4.207 ± 0.034

6.777 ± 0.038

1.434 ± 0.016

2.42 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski