Acidovorax sp. SLBN-42

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Acidovorax; unclassified Acidovorax

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A542J5D8|A0A542J5D8_9BURK Biotin-dependent carboxylase-like uncharacterized protein OS=Acidovorax sp. SLBN-42 OX=2768435 GN=FBY15_1682 PE=4 SV=1
MM1 pKa = 7.02SAKK4 pKa = 9.19TGRR7 pKa = 11.84VRR9 pKa = 11.84SIGRR13 pKa = 11.84FSALVGATAVVAALGGCAQGGPVGFDD39 pKa = 3.3GVQSATIQLEE49 pKa = 4.08AVGTFVSPEE58 pKa = 3.75EE59 pKa = 4.31GGYY62 pKa = 9.39EE63 pKa = 3.71AAGRR67 pKa = 11.84GSGFIITSDD76 pKa = 3.03GLAITNNHH84 pKa = 5.19VVTGAGTLKK93 pKa = 10.3AWRR96 pKa = 11.84GGDD99 pKa = 3.39TTNEE103 pKa = 3.57LSAKK107 pKa = 9.88VLGSSEE113 pKa = 4.32CLDD116 pKa = 3.65LAVIQLEE123 pKa = 4.35SGTYY127 pKa = 9.97PFLAWRR133 pKa = 11.84DD134 pKa = 3.58DD135 pKa = 4.64DD136 pKa = 5.39IEE138 pKa = 4.32TALDD142 pKa = 3.55VYY144 pKa = 11.28AAGFPLGDD152 pKa = 3.52PTFTEE157 pKa = 4.33TKK159 pKa = 10.46GIVSKK164 pKa = 11.33ANTNGEE170 pKa = 4.42TPWASIDD177 pKa = 3.73GVIEE181 pKa = 3.46HH182 pKa = 7.56DD183 pKa = 3.26ARR185 pKa = 11.84IRR187 pKa = 11.84GGNSGGPLVDD197 pKa = 3.02TDD199 pKa = 3.41GRR201 pKa = 11.84LVGVNYY207 pKa = 10.54AGNDD211 pKa = 3.53EE212 pKa = 5.19LDD214 pKa = 3.56YY215 pKa = 11.32NYY217 pKa = 10.57AIHH220 pKa = 7.35RR221 pKa = 11.84DD222 pKa = 3.45AVLEE226 pKa = 4.3VVSDD230 pKa = 3.89LVDD233 pKa = 3.99GKK235 pKa = 10.72DD236 pKa = 3.61VLSLGINGEE245 pKa = 4.07ALTDD249 pKa = 3.99DD250 pKa = 4.35EE251 pKa = 4.84GTGLGVWVNSVEE263 pKa = 4.35SGSAADD269 pKa = 3.75EE270 pKa = 4.73AGVEE274 pKa = 4.21PGDD277 pKa = 3.78LLTRR281 pKa = 11.84MEE283 pKa = 4.63GVSLAANGTMTEE295 pKa = 4.17YY296 pKa = 11.06CDD298 pKa = 3.38VLRR301 pKa = 11.84THH303 pKa = 6.51GQDD306 pKa = 2.94DD307 pKa = 3.99TLAVEE312 pKa = 5.1LYY314 pKa = 7.99RR315 pKa = 11.84TSEE318 pKa = 3.77DD319 pKa = 2.87AYY321 pKa = 11.5YY322 pKa = 10.51RR323 pKa = 11.84GQFNGDD329 pKa = 4.16PITAVPVVGGGGTNEE344 pKa = 3.81PSGDD348 pKa = 3.9YY349 pKa = 9.62VTVTDD354 pKa = 4.68DD355 pKa = 3.44SQTITVEE362 pKa = 4.55VPTAWSQVDD371 pKa = 4.25GAAVPPWEE379 pKa = 4.75SVTAAPDD386 pKa = 3.34LAGYY390 pKa = 7.3RR391 pKa = 11.84TTWATPGVTVYY402 pKa = 10.86ASQTAGVTPDD412 pKa = 2.98EE413 pKa = 4.52AANLVTEE420 pKa = 4.52GLAAQGCTSAGRR432 pKa = 11.84EE433 pKa = 4.01PYY435 pKa = 10.66DD436 pKa = 3.93DD437 pKa = 3.84GLYY440 pKa = 10.64AGVWEE445 pKa = 4.76IFTGCGGQATYY456 pKa = 11.19VVIGANDD463 pKa = 3.52YY464 pKa = 11.4DD465 pKa = 4.02GTHH468 pKa = 6.91TIVFTGQAITDD479 pKa = 3.92ADD481 pKa = 4.84LDD483 pKa = 4.36ALDD486 pKa = 3.72QAIGTFYY493 pKa = 11.05AAYY496 pKa = 10.21

Molecular weight: 51.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A542J1A6|A0A542J1A6_9BURK ABC-2 type transport system ATP-binding protein OS=Acidovorax sp. SLBN-42 OX=2768435 GN=FBY15_0173 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4383

0

4383

1418679

29

2104

323.7

34.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.909 ± 0.048

0.483 ± 0.008

6.2 ± 0.031

5.946 ± 0.03

3.163 ± 0.019

9.056 ± 0.032

2.055 ± 0.018

4.377 ± 0.028

1.67 ± 0.022

10.119 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.78 ± 0.014

1.839 ± 0.016

5.526 ± 0.027

2.532 ± 0.021

7.616 ± 0.045

5.331 ± 0.027

5.775 ± 0.027

9.105 ± 0.036

1.549 ± 0.016

1.969 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski