Mycoplasma agalactiae (strain PG2)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis; Mycoplasmopsis agalactiae

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5IYA6|A5IYA6_MYCAP Glucose 6 phosphate isomerase OS=Mycoplasma agalactiae (strain PG2) OX=347257 GN=pgiB PE=4 SV=1
MM1 pKa = 7.46SKK3 pKa = 10.56KK4 pKa = 10.23NKK6 pKa = 9.71NSSIYY11 pKa = 10.26YY12 pKa = 10.05DD13 pKa = 3.76NNDD16 pKa = 2.96FSNIVDD22 pKa = 3.44LHH24 pKa = 6.4GFTIPEE30 pKa = 4.5ALTQVQLSLANAYY43 pKa = 9.88EE44 pKa = 4.02DD45 pKa = 3.93DD46 pKa = 3.93FYY48 pKa = 11.71YY49 pKa = 10.7DD50 pKa = 3.62YY51 pKa = 11.21VTIITGKK58 pKa = 10.01GQGALMANIEE68 pKa = 4.22EE69 pKa = 4.39YY70 pKa = 11.01LRR72 pKa = 11.84DD73 pKa = 3.42SNYY76 pKa = 10.83DD77 pKa = 3.49YY78 pKa = 11.11EE79 pKa = 4.94ISFDD83 pKa = 3.7DD84 pKa = 4.56ASIKK88 pKa = 10.79VYY90 pKa = 10.82LL91 pKa = 4.17

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5IYX1|A5IYX1_MYCAP 50S ribosomal protein L6 OS=Mycoplasma agalactiae (strain PG2) OX=347257 GN=rplF PE=3 SV=1
MM1 pKa = 7.75ANPTQIYY8 pKa = 10.08SRR10 pKa = 11.84DD11 pKa = 3.6VKK13 pKa = 9.45WRR15 pKa = 11.84KK16 pKa = 9.32GVMRR20 pKa = 11.84TLVSEE25 pKa = 4.26LFANGRR31 pKa = 11.84ITTTLTRR38 pKa = 11.84AKK40 pKa = 9.75EE41 pKa = 4.06LRR43 pKa = 11.84RR44 pKa = 11.84HH45 pKa = 5.31AEE47 pKa = 3.84KK48 pKa = 10.9LITKK52 pKa = 9.61AKK54 pKa = 10.49NPTLANRR61 pKa = 11.84RR62 pKa = 11.84ICASFLRR69 pKa = 11.84PTLVEE74 pKa = 4.04EE75 pKa = 4.59GKK77 pKa = 10.66KK78 pKa = 10.45DD79 pKa = 3.73VLKK82 pKa = 11.03HH83 pKa = 5.81LFDD86 pKa = 5.28TIAPSYY92 pKa = 10.51AKK94 pKa = 10.49RR95 pKa = 11.84NGGYY99 pKa = 7.41TRR101 pKa = 11.84IYY103 pKa = 10.69KK104 pKa = 10.01LVNRR108 pKa = 11.84QGDD111 pKa = 4.15NAPMAIIEE119 pKa = 4.28LVV121 pKa = 3.11

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

726

0

726

250301

37

3329

344.8

39.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.907 ± 0.072

0.564 ± 0.021

5.712 ± 0.063

6.771 ± 0.086

5.242 ± 0.076

4.789 ± 0.069

1.515 ± 0.031

8.78 ± 0.087

10.326 ± 0.104

9.394 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.865 ± 0.04

7.486 ± 0.097

2.783 ± 0.048

2.778 ± 0.041

2.985 ± 0.052

7.323 ± 0.078

4.825 ± 0.049

5.848 ± 0.063

0.962 ± 0.032

4.144 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski