Beihai mantis shrimp virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJM5|A0A1L3KJM5_9VIRU Uncharacterized protein OS=Beihai mantis shrimp virus 1 OX=1922428 PE=4 SV=1
MM1 pKa = 7.81AFTEE5 pKa = 4.6LGWLTVKK12 pKa = 10.76KK13 pKa = 9.23LTQAPRR19 pKa = 11.84GLVVLLTTSLVVQQLMFLAVLNFNSLVMNQTAIIVEE55 pKa = 4.11NHH57 pKa = 5.98KK58 pKa = 11.23SLMEE62 pKa = 4.93DD63 pKa = 3.07LTSTQEE69 pKa = 4.15EE70 pKa = 4.42IASGITKK77 pKa = 10.3LDD79 pKa = 3.57GNVMEE84 pKa = 5.2FQLEE88 pKa = 4.48YY89 pKa = 9.78TNHH92 pKa = 5.98WPKK95 pKa = 10.85LDD97 pKa = 3.99PDD99 pKa = 4.21EE100 pKa = 4.82KK101 pKa = 10.81DD102 pKa = 2.91KK103 pKa = 11.45EE104 pKa = 4.5YY105 pKa = 11.22VSRR108 pKa = 11.84EE109 pKa = 3.86DD110 pKa = 3.4FDD112 pKa = 4.4EE113 pKa = 4.67FKK115 pKa = 11.14KK116 pKa = 10.88NYY118 pKa = 8.64AKK120 pKa = 10.44RR121 pKa = 11.84WLAEE125 pKa = 3.8PEE127 pKa = 4.23GEE129 pKa = 4.21YY130 pKa = 9.4VTSIKK135 pKa = 10.33FVEE138 pKa = 4.56KK139 pKa = 10.07LDD141 pKa = 3.83EE142 pKa = 4.77VIALLKK148 pKa = 10.68VPLVEE153 pKa = 4.27KK154 pKa = 10.75LNAVVEE160 pKa = 4.48TMEE163 pKa = 5.06FPACQTVSWDD173 pKa = 3.82GVLCEE178 pKa = 4.86NDD180 pKa = 3.31PFGIGPINCDD190 pKa = 2.92EE191 pKa = 4.18VVYY194 pKa = 10.84EE195 pKa = 4.39GTTCEE200 pKa = 4.01KK201 pKa = 10.51PVFIGII207 pKa = 4.02

Molecular weight: 23.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJR8|A0A1L3KJR8_9VIRU Uncharacterized protein OS=Beihai mantis shrimp virus 1 OX=1922428 PE=4 SV=1
MM1 pKa = 7.54AFSFTWNKK9 pKa = 9.74LVQSKK14 pKa = 9.21MEE16 pKa = 4.12SSKK19 pKa = 11.42ALDD22 pKa = 3.33SDD24 pKa = 3.95FTAGKK29 pKa = 9.97RR30 pKa = 11.84EE31 pKa = 3.89VVLTSGALPASYY43 pKa = 9.76EE44 pKa = 4.16VKK46 pKa = 9.53VQKK49 pKa = 8.45TVRR52 pKa = 11.84ITGTDD57 pKa = 3.23NIGDD61 pKa = 3.91LKK63 pKa = 11.05LFLSLQPSIDD73 pKa = 3.7AACEE77 pKa = 3.98EE78 pKa = 3.87IAKK81 pKa = 10.14RR82 pKa = 11.84YY83 pKa = 8.11EE84 pKa = 4.1RR85 pKa = 11.84YY86 pKa = 10.58SIDD89 pKa = 4.03NANLWVQTASPLGTSSGGFQLAHH112 pKa = 6.63IPDD115 pKa = 4.46PEE117 pKa = 4.24NANLPAADD125 pKa = 4.17PANATKK131 pKa = 10.41AVRR134 pKa = 11.84QQGSSFIRR142 pKa = 11.84PRR144 pKa = 11.84DD145 pKa = 3.53SVAFPIPLQGQLYY158 pKa = 7.81TKK160 pKa = 10.4QAGSQRR166 pKa = 11.84WWSFGAIVGVVRR178 pKa = 11.84AVPDD182 pKa = 3.22ATDD185 pKa = 3.54YY186 pKa = 12.0VEE188 pKa = 4.78FTCTFTATFRR198 pKa = 11.84FMRR201 pKa = 11.84TAEE204 pKa = 3.72VDD206 pKa = 3.14NNQRR210 pKa = 11.84VAFRR214 pKa = 11.84TLISEE219 pKa = 4.19ATVEE223 pKa = 4.07EE224 pKa = 4.51GRR226 pKa = 11.84LRR228 pKa = 11.84LRR230 pKa = 11.84TTAPVPGRR238 pKa = 11.84RR239 pKa = 11.84GTVTLPRR246 pKa = 11.84PAAVEE251 pKa = 3.93VRR253 pKa = 11.84DD254 pKa = 3.8RR255 pKa = 11.84SARR258 pKa = 11.84GVIVSHH264 pKa = 6.39SRR266 pKa = 11.84TATFDD271 pKa = 4.09CIIEE275 pKa = 4.41GQCATSVRR283 pKa = 11.84DD284 pKa = 3.94DD285 pKa = 3.99FVALHH290 pKa = 6.2TPLTNSTCLFQLQRR304 pKa = 11.84TFYY307 pKa = 10.29CQQ309 pKa = 2.8

Molecular weight: 34.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3447

113

2318

574.5

64.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.253 ± 0.797

2.176 ± 0.414

5.86 ± 0.401

7.079 ± 0.813

4.352 ± 0.212

5.976 ± 0.4

2.263 ± 0.448

4.584 ± 0.105

6.934 ± 1.218

8.326 ± 0.464

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.467

4.41 ± 0.58

4.062 ± 0.409

4.439 ± 0.384

5.628 ± 0.756

5.947 ± 0.417

5.454 ± 1.296

8.239 ± 0.44

1.247 ± 0.114

3.22 ± 0.582
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski