Tortoise microvirus 25

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W632|A0A4P8W632_9VIRU Major capsid protein OS=Tortoise microvirus 25 OX=2583127 PE=3 SV=1
MM1 pKa = 7.17TVDD4 pKa = 3.85SVVVTADD11 pKa = 3.28PHH13 pKa = 7.22DD14 pKa = 4.99DD15 pKa = 3.69EE16 pKa = 5.98VVTDD20 pKa = 3.79PAGYY24 pKa = 9.5IPKK27 pKa = 10.03EE28 pKa = 3.86EE29 pKa = 3.99QLRR32 pKa = 11.84ILDD35 pKa = 3.72EE36 pKa = 4.84AGVNLEE42 pKa = 3.59QYY44 pKa = 9.07YY45 pKa = 10.16RR46 pKa = 11.84QMYY49 pKa = 9.31PNASYY54 pKa = 11.05TDD56 pKa = 3.83ADD58 pKa = 4.16DD59 pKa = 6.09DD60 pKa = 4.78VYY62 pKa = 11.67EE63 pKa = 4.25CDD65 pKa = 3.15KK66 pKa = 11.17YY67 pKa = 11.32GRR69 pKa = 11.84DD70 pKa = 3.59EE71 pKa = 4.21MDD73 pKa = 3.44VLDD76 pKa = 4.44EE77 pKa = 3.99MRR79 pKa = 11.84EE80 pKa = 4.11AVDD83 pKa = 3.24NLSRR87 pKa = 11.84RR88 pKa = 11.84VEE90 pKa = 4.05DD91 pKa = 4.23LEE93 pKa = 4.96EE94 pKa = 4.39GAPPPKK100 pKa = 10.05VDD102 pKa = 3.27NVTSQGTVVDD112 pKa = 4.13NQPPMSTPQDD122 pKa = 3.73NTPSPPP128 pKa = 3.21

Molecular weight: 14.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W5Y3|A0A4P8W5Y3_9VIRU Uncharacterized protein OS=Tortoise microvirus 25 OX=2583127 PE=4 SV=1
MM1 pKa = 7.79DD2 pKa = 6.42PIVTGAIIGAGAHH15 pKa = 6.47IGTNLLGMGHH25 pKa = 6.83AAKK28 pKa = 9.41MDD30 pKa = 3.68KK31 pKa = 10.83KK32 pKa = 10.76NLEE35 pKa = 3.96QRR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.89EE40 pKa = 4.04YY41 pKa = 10.92AEE43 pKa = 4.45HH44 pKa = 7.17KK45 pKa = 10.46RR46 pKa = 11.84QFDD49 pKa = 3.88LNYY52 pKa = 9.96QHH54 pKa = 6.49QQRR57 pKa = 11.84QYY59 pKa = 8.13THH61 pKa = 7.27GIRR64 pKa = 11.84DD65 pKa = 3.49RR66 pKa = 11.84VADD69 pKa = 4.02LTAAGLHH76 pKa = 5.67PTLAAGQPASAPMAVSGGSPKK97 pKa = 9.55STPLRR102 pKa = 11.84SQRR105 pKa = 11.84AEE107 pKa = 4.44SIMQMAAQVQNIANIAAQNRR127 pKa = 11.84LLRR130 pKa = 11.84AQAAHH135 pKa = 6.0VNADD139 pKa = 3.77TEE141 pKa = 4.42RR142 pKa = 11.84VKK144 pKa = 11.29ADD146 pKa = 3.29TEE148 pKa = 4.32GLKK151 pKa = 10.74GRR153 pKa = 11.84EE154 pKa = 3.9TRR156 pKa = 11.84SQEE159 pKa = 3.67MHH161 pKa = 7.0ALEE164 pKa = 4.19QQLKK168 pKa = 8.76RR169 pKa = 11.84AGIRR173 pKa = 11.84AHH175 pKa = 5.73QAQALVKK182 pKa = 9.13EE183 pKa = 4.41IEE185 pKa = 4.25GKK187 pKa = 9.84YY188 pKa = 6.51MQRR191 pKa = 11.84EE192 pKa = 4.68GMRR195 pKa = 11.84MPQRR199 pKa = 11.84RR200 pKa = 11.84PEE202 pKa = 3.84IGEE205 pKa = 3.74IIAAIEE211 pKa = 3.94RR212 pKa = 11.84VIPLLPRR219 pKa = 11.84QQITNLAIDD228 pKa = 3.24IRR230 pKa = 11.84DD231 pKa = 5.02FIRR234 pKa = 11.84DD235 pKa = 3.39TGVSAKK241 pKa = 10.33EE242 pKa = 4.03SFHH245 pKa = 6.45EE246 pKa = 4.39VTRR249 pKa = 11.84PGGTIDD255 pKa = 3.24QNRR258 pKa = 11.84ASRR261 pKa = 11.84RR262 pKa = 11.84EE263 pKa = 3.64NWEE266 pKa = 3.32RR267 pKa = 11.84EE268 pKa = 3.57QRR270 pKa = 11.84YY271 pKa = 8.85RR272 pKa = 11.84EE273 pKa = 4.06SQQRR277 pKa = 11.84RR278 pKa = 11.84RR279 pKa = 11.84NQQ281 pKa = 2.84

Molecular weight: 31.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1403

95

590

280.6

31.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.413 ± 1.33

1.354 ± 0.685

5.631 ± 1.437

5.845 ± 0.702

3.279 ± 0.773

6.842 ± 0.594

2.067 ± 0.398

5.773 ± 0.595

4.063 ± 1.109

6.7 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.136 ± 0.183

4.633 ± 0.754

5.702 ± 0.73

5.203 ± 1.34

8.553 ± 0.972

5.702 ± 0.655

5.773 ± 0.752

6.13 ± 1.041

1.14 ± 0.386

5.061 ± 1.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski