Paraliobacillus sp. PM-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Paraliobacillus; unclassified Paraliobacillus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2774 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1KME7|A0A0U1KME7_9BACI Beta-galactosidase OS=Paraliobacillus sp. PM-2 OX=1462524 GN=bga_2 PE=3 SV=1
MM1 pKa = 7.49EE2 pKa = 6.37SITILYY8 pKa = 10.84ASMTGNTEE16 pKa = 3.91EE17 pKa = 3.8IAEE20 pKa = 4.42IIEE23 pKa = 4.54SYY25 pKa = 10.47IEE27 pKa = 3.78KK28 pKa = 10.44QGIKK32 pKa = 9.72VEE34 pKa = 4.14RR35 pKa = 11.84FQIGFDD41 pKa = 3.51GFEE44 pKa = 3.95VEE46 pKa = 5.9QLFEE50 pKa = 4.23TDD52 pKa = 3.32GVLIGTYY59 pKa = 9.72TDD61 pKa = 3.91GDD63 pKa = 3.6GDD65 pKa = 4.35LPFEE69 pKa = 5.23AEE71 pKa = 4.06DD72 pKa = 4.86LYY74 pKa = 11.65DD75 pKa = 3.98EE76 pKa = 5.78LEE78 pKa = 4.15EE79 pKa = 5.96LDD81 pKa = 3.6LTGIPFGLFGSCDD94 pKa = 3.23SMYY97 pKa = 10.28TFYY100 pKa = 11.25GGAIDD105 pKa = 4.76SFEE108 pKa = 4.3TLVKK112 pKa = 10.39EE113 pKa = 4.25CGANIVVPTLKK124 pKa = 10.11IEE126 pKa = 4.36LSPEE130 pKa = 3.41IEE132 pKa = 4.73DD133 pKa = 3.99IKK135 pKa = 9.44TCEE138 pKa = 3.93SFAATFIEE146 pKa = 4.74KK147 pKa = 9.97SQKK150 pKa = 10.31

Molecular weight: 16.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1KIX8|A0A0U1KIX8_9BACI 3-mercaptopyruvate sulfurtransferase OS=Paraliobacillus sp. PM-2 OX=1462524 GN=sseA PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84KK13 pKa = 8.32KK14 pKa = 8.57VHH16 pKa = 5.61GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 9.12VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.99KK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2774

0

2774

837727

29

2282

302.0

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.65 ± 0.056

0.609 ± 0.012

5.591 ± 0.049

7.344 ± 0.057

4.406 ± 0.042

6.341 ± 0.039

2.138 ± 0.023

8.298 ± 0.052

6.945 ± 0.046

9.431 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.718 ± 0.022

4.926 ± 0.042

3.402 ± 0.027

4.299 ± 0.04

3.724 ± 0.037

5.91 ± 0.036

5.754 ± 0.033

6.782 ± 0.036

1.009 ± 0.017

3.725 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski