Escherichia phage N30

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus; Escherichia virus N30

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386K816|A0A386K816_9CAUD DNA ligase OS=Escherichia phage N30 OX=2340719 PE=4 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 11.18NNVFDD13 pKa = 4.72HH14 pKa = 6.93AYY16 pKa = 10.49EE17 pKa = 3.86MLKK20 pKa = 10.85EE21 pKa = 4.08NIRR24 pKa = 11.84YY25 pKa = 9.81DD26 pKa = 4.67DD27 pKa = 4.5IRR29 pKa = 11.84DD30 pKa = 3.54TDD32 pKa = 3.84DD33 pKa = 3.34LHH35 pKa = 8.58DD36 pKa = 4.36VVHH39 pKa = 6.41MAADD43 pKa = 3.63SAVPHH48 pKa = 6.4YY49 pKa = 10.5YY50 pKa = 10.65SDD52 pKa = 3.44IFSVMASEE60 pKa = 5.55GIDD63 pKa = 4.5LEE65 pKa = 4.76FEE67 pKa = 4.73DD68 pKa = 5.02SGLMPDD74 pKa = 3.4TKK76 pKa = 10.94DD77 pKa = 3.64VIRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 9.25EE88 pKa = 4.14QLTIDD93 pKa = 3.64LWEE96 pKa = 4.69DD97 pKa = 3.57AEE99 pKa = 4.86DD100 pKa = 4.13LLNEE104 pKa = 3.99YY105 pKa = 10.5LEE107 pKa = 4.28EE108 pKa = 4.44VEE110 pKa = 5.21AEE112 pKa = 4.1EE113 pKa = 5.83DD114 pKa = 3.68EE115 pKa = 4.57EE116 pKa = 4.44

Molecular weight: 13.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386K7B0|A0A386K7B0_9CAUD Endolysin OS=Escherichia phage N30 OX=2340719 PE=3 SV=1
MM1 pKa = 7.61TEE3 pKa = 4.07LTDD6 pKa = 4.0GLKK9 pKa = 10.21KK10 pKa = 10.77GYY12 pKa = 7.09MTNGTLYY19 pKa = 10.19AANRR23 pKa = 11.84RR24 pKa = 11.84IVRR27 pKa = 11.84TWRR30 pKa = 11.84EE31 pKa = 3.6NNLEE35 pKa = 3.8RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.88AQEE41 pKa = 3.05RR42 pKa = 11.84AAWHH46 pKa = 5.58RR47 pKa = 11.84RR48 pKa = 11.84KK49 pKa = 10.42EE50 pKa = 3.97KK51 pKa = 11.0VKK53 pKa = 10.57AKK55 pKa = 10.4KK56 pKa = 9.85LAALEE61 pKa = 4.04KK62 pKa = 10.84ALANTLNAISS72 pKa = 3.67

Molecular weight: 8.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

11705

51

1318

266.0

29.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.252 ± 0.483

1.059 ± 0.203

6.493 ± 0.268

7.416 ± 0.385

3.665 ± 0.241

7.475 ± 0.301

1.956 ± 0.223

5.288 ± 0.178

6.698 ± 0.395

7.911 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.059 ± 0.254

4.468 ± 0.26

3.494 ± 0.231

3.751 ± 0.369

5.63 ± 0.224

5.792 ± 0.28

5.51 ± 0.236

6.254 ± 0.258

1.606 ± 0.168

3.221 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski