Brachybacterium phenoliresistens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermabacteraceae; Brachybacterium

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3605 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Z9JRZ1|Z9JRZ1_9MICO RNA polymerase subunit sigma-24 OS=Brachybacterium phenoliresistens OX=396014 GN=BF93_02760 PE=3 SV=1
MM1 pKa = 7.6HH2 pKa = 7.81DD3 pKa = 3.61VRR5 pKa = 11.84HH6 pKa = 5.59GRR8 pKa = 11.84APRR11 pKa = 11.84AMRR14 pKa = 11.84YY15 pKa = 8.77LATAAAVLLVVAGCGPLDD33 pKa = 4.3RR34 pKa = 11.84IGIGGGGDD42 pKa = 3.61EE43 pKa = 4.49QTTAPAAGDD52 pKa = 3.5GTNAEE57 pKa = 4.63GEE59 pKa = 4.38ATPPLAEE66 pKa = 4.55TGTSAAAEE74 pKa = 4.32LPQDD78 pKa = 3.81PAGDD82 pKa = 3.56AAYY85 pKa = 10.0AAYY88 pKa = 10.49YY89 pKa = 8.75SQQVAWGPCDD99 pKa = 4.55DD100 pKa = 5.0SVDD103 pKa = 3.84GPASLEE109 pKa = 4.32CGTVTVPKK117 pKa = 10.25VWNDD121 pKa = 3.18PSAGDD126 pKa = 3.53LQIAVTRR133 pKa = 11.84LPATGQKK140 pKa = 9.8QGSLLTNPGGPGGSGVDD157 pKa = 4.41FVAGSAQTLFSEE169 pKa = 5.05EE170 pKa = 3.38VRR172 pKa = 11.84GAYY175 pKa = 10.08DD176 pKa = 3.05IVGFDD181 pKa = 3.43PRR183 pKa = 11.84GVSRR187 pKa = 11.84SAGITCLDD195 pKa = 3.89DD196 pKa = 5.28AEE198 pKa = 4.33TDD200 pKa = 3.17AYY202 pKa = 10.63RR203 pKa = 11.84AEE205 pKa = 4.39TFDD208 pKa = 4.13LADD211 pKa = 3.96PEE213 pKa = 4.4GLQAAEE219 pKa = 4.27DD220 pKa = 3.96AFTEE224 pKa = 5.0LSAACEE230 pKa = 4.16KK231 pKa = 11.05NSGDD235 pKa = 4.05LLPYY239 pKa = 10.36LDD241 pKa = 4.56TYY243 pKa = 11.16SAARR247 pKa = 11.84DD248 pKa = 3.52LDD250 pKa = 3.8VLRR253 pKa = 11.84SAVGSEE259 pKa = 3.45KK260 pKa = 10.42LDD262 pKa = 3.63YY263 pKa = 10.95VGYY266 pKa = 10.6SYY268 pKa = 10.44GTYY271 pKa = 10.47LGATYY276 pKa = 11.16AEE278 pKa = 4.83MYY280 pKa = 9.81PEE282 pKa = 3.79RR283 pKa = 11.84TGRR286 pKa = 11.84FVLDD290 pKa = 3.6GALDD294 pKa = 3.78PRR296 pKa = 11.84LTADD300 pKa = 5.23DD301 pKa = 3.58ITRR304 pKa = 11.84GQAEE308 pKa = 4.46GFEE311 pKa = 4.5EE312 pKa = 4.12ATSAFVEE319 pKa = 4.66SCLSQGSDD327 pKa = 3.31GCPLTGSVEE336 pKa = 4.17DD337 pKa = 4.0GKK339 pKa = 10.85EE340 pKa = 3.62QLRR343 pKa = 11.84AFFDD347 pKa = 3.86SVDD350 pKa = 3.7AQPLQTDD357 pKa = 3.8DD358 pKa = 4.04PNRR361 pKa = 11.84PLTGALARR369 pKa = 11.84SGLLVVLYY377 pKa = 10.7NDD379 pKa = 3.96EE380 pKa = 4.09NWPLGVQALNAAMTGDD396 pKa = 3.57GSTLLFLADD405 pKa = 4.21LSADD409 pKa = 3.96RR410 pKa = 11.84EE411 pKa = 4.24DD412 pKa = 4.4DD413 pKa = 3.33GTYY416 pKa = 10.54SGNGTFAITAVNCLDD431 pKa = 3.64HH432 pKa = 8.2VGVADD437 pKa = 3.92QQWQIDD443 pKa = 3.77EE444 pKa = 4.3AAAVAEE450 pKa = 4.8EE451 pKa = 4.45FPTWGPGFGFSQTMCDD467 pKa = 3.5LWPAKK472 pKa = 9.42PVRR475 pKa = 11.84EE476 pKa = 4.08PAPISAAGSDD486 pKa = 4.28LIVVIGTRR494 pKa = 11.84GDD496 pKa = 3.38PATPYY501 pKa = 10.55DD502 pKa = 3.56WAVGLDD508 pKa = 3.6EE509 pKa = 4.28QLEE512 pKa = 4.22NSTLITYY519 pKa = 9.63EE520 pKa = 4.34GEE522 pKa = 3.66GHH524 pKa = 5.38TAYY527 pKa = 10.17GRR529 pKa = 11.84SGGCVEE535 pKa = 4.59AAVDD539 pKa = 4.26AYY541 pKa = 10.68LLSGTAPEE549 pKa = 4.62EE550 pKa = 4.25GLTCSS555 pKa = 3.97

Molecular weight: 57.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Z9JQY5|Z9JQY5_9MICO Ribosome hibernation promoting factor OS=Brachybacterium phenoliresistens OX=396014 GN=hpf PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPNTRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.1KK16 pKa = 8.61HH17 pKa = 4.4GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIINARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.68GRR40 pKa = 11.84AEE42 pKa = 4.11LSAA45 pKa = 4.93

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3605

0

3605

1230214

37

2216

341.3

36.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.153 ± 0.06

0.565 ± 0.009

6.054 ± 0.037

6.159 ± 0.04

2.686 ± 0.023

9.336 ± 0.036

2.182 ± 0.021

4.092 ± 0.026

1.346 ± 0.025

10.6 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.914 ± 0.017

1.416 ± 0.019

6.109 ± 0.032

2.836 ± 0.021

7.982 ± 0.047

5.361 ± 0.03

5.654 ± 0.029

8.326 ± 0.037

1.455 ± 0.016

1.774 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski