Bifidobacterium cuniculi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087AKI5|A0A087AKI5_9BIFI Transposase for IS3509a (Fragment) OS=Bifidobacterium cuniculi OX=1688 GN=BCUN_1626 PE=4 SV=1
MM1 pKa = 7.61SNPYY5 pKa = 10.96DD6 pKa = 3.82NGTQSTPSNPYY17 pKa = 8.23QSSPVPHH24 pKa = 7.07ANDD27 pKa = 4.78DD28 pKa = 3.85NTSAPQQFPSQLNYY42 pKa = 10.02GQSAVPDD49 pKa = 3.57ATQAFPAPGRR59 pKa = 11.84TVPVDD64 pKa = 3.14ATQPLTGTGQQPFPYY79 pKa = 9.34GGQPAPTYY87 pKa = 8.33GQPPADD93 pKa = 4.33PTQAMPPYY101 pKa = 8.54GQPPAGAYY109 pKa = 8.36PGQPGQPQVVQSGQGQEE126 pKa = 3.97QPTTEE131 pKa = 3.9LSGYY135 pKa = 7.61GQPQYY140 pKa = 11.06GQQPQYY146 pKa = 11.41GQMPPQIPPQNGYY159 pKa = 9.8EE160 pKa = 4.08PQPGGAPKK168 pKa = 10.3KK169 pKa = 9.47KK170 pKa = 9.87LHH172 pKa = 6.75PGVIAGVIVAAVVAIALIVWGIIALTGNDD201 pKa = 4.17DD202 pKa = 4.02AKK204 pKa = 11.2ADD206 pKa = 3.9PTASPSVSSSVPATSDD222 pKa = 3.41PAPSPKK228 pKa = 9.4TDD230 pKa = 3.49PTDD233 pKa = 3.62EE234 pKa = 4.34PTTDD238 pKa = 3.21IDD240 pKa = 6.15DD241 pKa = 4.45PFEE244 pKa = 5.89DD245 pKa = 5.42LDD247 pKa = 6.21DD248 pKa = 5.52DD249 pKa = 4.27LTGDD253 pKa = 3.71TLEE256 pKa = 5.7DD257 pKa = 3.81FLKK260 pKa = 9.97LTNADD265 pKa = 3.48EE266 pKa = 4.54EE267 pKa = 4.41FAKK270 pKa = 10.89LSDD273 pKa = 3.71QMPDD277 pKa = 3.37GVNMDD282 pKa = 3.94IYY284 pKa = 10.95ATGNTLVFDD293 pKa = 4.82LALDD297 pKa = 4.25DD298 pKa = 4.46FTGSTADD305 pKa = 3.46MMSDD309 pKa = 3.14MLVEE313 pKa = 4.01EE314 pKa = 5.28LEE316 pKa = 4.61PEE318 pKa = 4.31LKK320 pKa = 10.32EE321 pKa = 4.22QAGEE325 pKa = 4.06MQEE328 pKa = 4.14MFPDD332 pKa = 3.39AAMQLKK338 pKa = 10.09IVDD341 pKa = 3.98GDD343 pKa = 3.77GKK345 pKa = 8.64TLYY348 pKa = 10.94NEE350 pKa = 4.31TYY352 pKa = 10.26KK353 pKa = 11.1

Molecular weight: 37.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087ANE0|A0A087ANE0_9BIFI Oxidoreductase OS=Bifidobacterium cuniculi OX=1688 GN=BCUN_1454 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.79MKK15 pKa = 9.36HH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 6.57TLAAA44 pKa = 4.49

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2189

0

2189

724817

33

3027

331.1

36.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.481 ± 0.084

1.044 ± 0.018

7.067 ± 0.048

5.654 ± 0.056

3.147 ± 0.03

8.087 ± 0.047

2.337 ± 0.028

4.652 ± 0.039

3.219 ± 0.045

8.922 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.793 ± 0.026

2.813 ± 0.035

4.873 ± 0.037

3.72 ± 0.034

6.654 ± 0.06

5.29 ± 0.038

6.164 ± 0.049

7.944 ± 0.052

1.477 ± 0.02

2.661 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski