Methylobacterium sp. Leaf399

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; unclassified Methylobacterium

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3885 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5ZMA7|A0A0Q5ZMA7_9RHIZ Inositol monophosphatase OS=Methylobacterium sp. Leaf399 OX=1736364 GN=ASG40_18625 PE=4 SV=1
MM1 pKa = 6.91SQSVSNTQPSLVVKK15 pKa = 10.2SYY17 pKa = 9.16ITTQGLYY24 pKa = 7.73PTAGSGGPGTAPQNDD39 pKa = 4.24DD40 pKa = 3.39EE41 pKa = 5.05YY42 pKa = 10.59MGVIRR47 pKa = 11.84LTAASYY53 pKa = 11.32APGVDD58 pKa = 4.05VSGQTLNIRR67 pKa = 11.84GNEE70 pKa = 3.78ALYY73 pKa = 10.8SILGTRR79 pKa = 11.84YY80 pKa = 10.17GGDD83 pKa = 2.83GRR85 pKa = 11.84TTFKK89 pKa = 11.11LPDD92 pKa = 3.6FMGDD96 pKa = 3.09VSVGVGGGFLTGLGQHH112 pKa = 6.78GGTTATALTTANLPANLGGQGQTFDD137 pKa = 3.39NHH139 pKa = 5.95QSSEE143 pKa = 3.88AANYY147 pKa = 9.24LISVNGFFGTYY158 pKa = 9.49RR159 pKa = 11.84AGTLMQTAFDD169 pKa = 3.85IVPEE173 pKa = 4.43GFLPADD179 pKa = 3.4GRR181 pKa = 11.84LLNSADD187 pKa = 3.69YY188 pKa = 9.79PLLYY192 pKa = 10.4AALGTAYY199 pKa = 10.14GGDD202 pKa = 3.32GTTFRR207 pKa = 11.84LPDD210 pKa = 3.33LTGRR214 pKa = 11.84AVMGDD219 pKa = 3.59DD220 pKa = 3.39ASTVIGQQVGSATVSLTNANAPASAGGSGQAFANGQPALVMTYY263 pKa = 10.13LIAVQGLYY271 pKa = 9.9PDD273 pKa = 3.98RR274 pKa = 11.84DD275 pKa = 3.93GGRR278 pKa = 11.84GAYY281 pKa = 9.32SEE283 pKa = 4.66PYY285 pKa = 9.66IGEE288 pKa = 4.18LFAYY292 pKa = 10.13AGTDD296 pKa = 3.21VSDD299 pKa = 3.98ANLPEE304 pKa = 5.08GYY306 pKa = 10.15LRR308 pKa = 11.84AEE310 pKa = 4.39GQLLPINQYY319 pKa = 9.03QALYY323 pKa = 8.54TLIGATYY330 pKa = 8.95GGDD333 pKa = 3.65GISVFRR339 pKa = 11.84LPDD342 pKa = 3.08LRR344 pKa = 11.84GRR346 pKa = 11.84VAVDD350 pKa = 3.86DD351 pKa = 4.27GTDD354 pKa = 3.22RR355 pKa = 11.84PLGQTFGQAGEE366 pKa = 4.13TLDD369 pKa = 4.39ANEE372 pKa = 4.65IPAPAAPVITGPANGATTTDD392 pKa = 3.75DD393 pKa = 3.68TPDD396 pKa = 3.06ISGTASPNSIVTVVIDD412 pKa = 3.9GATVGTTRR420 pKa = 11.84ANASGAWTFTPTVKK434 pKa = 10.71LSDD437 pKa = 3.66AAHH440 pKa = 6.2SVTAAASYY448 pKa = 11.1GGDD451 pKa = 3.28QSPASTARR459 pKa = 11.84TFAVDD464 pKa = 3.74VNDD467 pKa = 4.21APALTGPQTALAAGSEE483 pKa = 4.17DD484 pKa = 3.13TAYY487 pKa = 9.79TVTAAQLLQGWTDD500 pKa = 3.4ADD502 pKa = 3.88FTTLAVTGLITSSGSVTSDD521 pKa = 2.81GNGTYY526 pKa = 9.79TVTPAANANGPVTLSYY542 pKa = 10.56QVSDD546 pKa = 3.48GVNAVSATRR555 pKa = 11.84TFTLAAINDD564 pKa = 3.99APQLTGPQATLPAGTEE580 pKa = 3.92DD581 pKa = 3.3TAFTVTSAQLLQGFSDD597 pKa = 4.09PEE599 pKa = 4.16GTTLVVSSLSVDD611 pKa = 3.04HH612 pKa = 6.24GTVVSDD618 pKa = 3.71GAGGYY623 pKa = 8.23TITPEE628 pKa = 3.81ANYY631 pKa = 10.24NGPLTISYY639 pKa = 10.61SVGDD643 pKa = 4.0GTASLPATLSTTLAAVNDD661 pKa = 4.02APRR664 pKa = 11.84LTGTQATLVVGTEE677 pKa = 3.96DD678 pKa = 3.12TAFTVTSAHH687 pKa = 6.79LLQGFSDD694 pKa = 4.31PDD696 pKa = 3.24GTTPVVLNLSVDD708 pKa = 3.24HH709 pKa = 6.17GTVVSDD715 pKa = 3.71GAGGYY720 pKa = 8.22TITPTANYY728 pKa = 9.21NGPLTISXSLSVDD741 pKa = 2.78HH742 pKa = 6.33GTVVSDD748 pKa = 3.71GAGGYY753 pKa = 8.22TITPTANYY761 pKa = 9.21NGPLTISYY769 pKa = 10.61SVGDD773 pKa = 4.11GTASTPATLSTAITAVNDD791 pKa = 3.67APRR794 pKa = 11.84LTDD797 pKa = 3.47TQATLADD804 pKa = 4.05GTEE807 pKa = 4.14DD808 pKa = 3.17TAFTVTSAQLLQGFSDD824 pKa = 3.91PDD826 pKa = 3.37GTTLVVSSLSVDD838 pKa = 3.04HH839 pKa = 6.24GTVVSDD845 pKa = 3.71GAGGYY850 pKa = 8.22TITPTANYY858 pKa = 9.21NGPLTISYY866 pKa = 10.61SVGDD870 pKa = 3.98GTASIPATLSTTLAAVNDD888 pKa = 3.97APRR891 pKa = 11.84LTGAQATLADD901 pKa = 4.13GTEE904 pKa = 4.2DD905 pKa = 3.25TTFTVTSAQLLQGFTDD921 pKa = 4.08PDD923 pKa = 3.33GTTPVLSNLSVDD935 pKa = 3.29HH936 pKa = 6.09GTVVSDD942 pKa = 3.71GAGGYY947 pKa = 8.22TITPTANYY955 pKa = 8.77NGPITISYY963 pKa = 10.49DD964 pKa = 3.24VGDD967 pKa = 4.31GVSSVAATRR976 pKa = 11.84DD977 pKa = 3.46VTIDD981 pKa = 3.48AVDD984 pKa = 4.06DD985 pKa = 4.47APVLAEE991 pKa = 4.74PGTVTLSEE999 pKa = 4.33SAAPGTTVATLSATDD1014 pKa = 3.76PDD1016 pKa = 4.3GAGVLTYY1023 pKa = 10.36AIQGGNQAGLFTIDD1037 pKa = 3.08ATTGVIALAAGRR1049 pKa = 11.84SVDD1052 pKa = 3.34AQTTARR1058 pKa = 11.84HH1059 pKa = 4.85VLGVRR1064 pKa = 11.84ATDD1067 pKa = 3.81GSGLVDD1073 pKa = 4.91DD1074 pKa = 5.47RR1075 pKa = 11.84SLTIDD1080 pKa = 3.42VLDD1083 pKa = 3.67VDD1085 pKa = 4.15RR1086 pKa = 11.84AGVLTLTGLTGGNARR1101 pKa = 11.84EE1102 pKa = 4.21GTPIVALLADD1112 pKa = 4.2ADD1114 pKa = 4.26EE1115 pKa = 4.59ATGAITYY1122 pKa = 7.51TFTDD1126 pKa = 3.26VSDD1129 pKa = 3.98PQAPRR1134 pKa = 11.84VLLTGSSASYY1144 pKa = 9.54TPDD1147 pKa = 2.86YY1148 pKa = 9.64TAAGQAIAVTVSYY1161 pKa = 10.94TDD1163 pKa = 3.82DD1164 pKa = 3.31QGHH1167 pKa = 6.44TGLASASAGVVGNVDD1182 pKa = 3.35VAPPQTQLVSGPAAVTRR1199 pKa = 11.84ATSATFTFSGSDD1211 pKa = 3.81DD1212 pKa = 3.58IAVDD1216 pKa = 3.7HH1217 pKa = 6.65YY1218 pKa = 11.06EE1219 pKa = 3.83VSLDD1223 pKa = 2.99GGGYY1227 pKa = 10.01VRR1229 pKa = 11.84TTSPYY1234 pKa = 9.59TVTGLADD1241 pKa = 3.93GVHH1244 pKa = 6.01SFAVRR1249 pKa = 11.84AVDD1252 pKa = 3.5AAGNADD1258 pKa = 4.32LTPEE1262 pKa = 4.15STLWTVDD1269 pKa = 3.18TQAPTVSGVSLSGPGIVRR1287 pKa = 11.84GSGTLTVGQSVIISVALSEE1306 pKa = 4.52PVTLTDD1312 pKa = 3.78AGGLRR1317 pKa = 11.84LQLSDD1322 pKa = 4.16GGVAAYY1328 pKa = 9.41DD1329 pKa = 3.68AAASDD1334 pKa = 3.77ASHH1337 pKa = 6.91LVFTHH1342 pKa = 5.49TVVVDD1347 pKa = 3.97AVSADD1352 pKa = 3.7LAITGLTLGSATILDD1367 pKa = 3.75AAGNVASLAGAATNPAGALRR1387 pKa = 11.84IDD1389 pKa = 5.09GYY1391 pKa = 9.62TGTAAAEE1398 pKa = 4.44TFHH1401 pKa = 6.07GTPGAEE1407 pKa = 4.06TFKK1410 pKa = 11.24GQGGNDD1416 pKa = 3.33TYY1418 pKa = 11.61LVDD1421 pKa = 3.72NVGDD1425 pKa = 3.8RR1426 pKa = 11.84VIEE1429 pKa = 4.07ATGGGSDD1436 pKa = 3.59SVIASVSYY1444 pKa = 10.62RR1445 pKa = 11.84LAAGQEE1451 pKa = 4.17IEE1453 pKa = 4.48SLALSTSTGGTALTLTGNEE1472 pKa = 4.15FANILTGNDD1481 pKa = 3.3GDD1483 pKa = 3.9NTLNGGLGADD1493 pKa = 3.91TMTGGAGSDD1502 pKa = 3.68FYY1504 pKa = 11.91VVDD1507 pKa = 4.14DD1508 pKa = 4.83AGDD1511 pKa = 3.52RR1512 pKa = 11.84AIEE1515 pKa = 3.95FAGLGTDD1522 pKa = 3.91LVYY1525 pKa = 10.41ATVSYY1530 pKa = 11.22SLGGSQLEE1538 pKa = 4.12NLTLAGTDD1546 pKa = 3.19NLTGLGNSLANVISGNGGDD1565 pKa = 3.73NVINGLGGADD1575 pKa = 3.47RR1576 pKa = 11.84MSGGAGSDD1584 pKa = 3.18TYY1586 pKa = 11.7YY1587 pKa = 11.18VDD1589 pKa = 4.09NLDD1592 pKa = 4.36DD1593 pKa = 4.41KK1594 pKa = 11.55VIEE1597 pKa = 4.08ATGAVGTDD1605 pKa = 3.05LVYY1608 pKa = 11.13ASVSYY1613 pKa = 11.04SLAGSHH1619 pKa = 6.99LEE1621 pKa = 3.82NLTLTGAGNLTATGNSLANVLTGNSGANVLDD1652 pKa = 3.87GLGGADD1658 pKa = 3.65TMSGGAGSDD1667 pKa = 3.21TYY1669 pKa = 11.74YY1670 pKa = 11.16VDD1672 pKa = 3.64TVDD1675 pKa = 5.8DD1676 pKa = 4.19RR1677 pKa = 11.84VLEE1680 pKa = 4.06ATGAVGTDD1688 pKa = 3.05LVYY1691 pKa = 11.13ASVSYY1696 pKa = 10.99SLAGAHH1702 pKa = 6.7LEE1704 pKa = 3.88NLTLTGAGNLTATGNSLANVITGTRR1729 pKa = 11.84GANILDD1735 pKa = 3.95GQGGDD1740 pKa = 4.86DD1741 pKa = 4.2ILTGGAGADD1750 pKa = 3.14TFVFKK1755 pKa = 9.98TALGSGNVDD1764 pKa = 4.04HH1765 pKa = 6.92ITDD1768 pKa = 4.72FVASTAAVHH1777 pKa = 6.48DD1778 pKa = 4.37TVQLARR1784 pKa = 11.84SVFATLAAGQLTEE1797 pKa = 5.1AAFKK1801 pKa = 10.96DD1802 pKa = 4.07SGNVDD1807 pKa = 4.25HH1808 pKa = 6.99ITDD1811 pKa = 4.72FVASTAAVHH1820 pKa = 6.48DD1821 pKa = 4.37TVQLARR1827 pKa = 11.84SVFATLAAGQLTEE1840 pKa = 5.3AAFKK1844 pKa = 11.0DD1845 pKa = 3.98LGVSAADD1852 pKa = 3.16ASDD1855 pKa = 4.31RR1856 pKa = 11.84ILYY1859 pKa = 10.33DD1860 pKa = 3.13KK1861 pKa = 10.68GTGGLFYY1868 pKa = 10.82DD1869 pKa = 4.32ADD1871 pKa = 4.28GSGSAATAVQFAVIDD1886 pKa = 3.88TKK1888 pKa = 10.82VTLSHH1893 pKa = 7.01HH1894 pKa = 6.78DD1895 pKa = 3.58FFVVV1899 pKa = 3.31

Molecular weight: 189.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6A4Z3|A0A0Q6A4Z3_9RHIZ Large-conductance mechanosensitive channel OS=Methylobacterium sp. Leaf399 OX=1736364 GN=mscL PE=3 SV=1
MM1 pKa = 6.73TTPFSSRR8 pKa = 11.84RR9 pKa = 11.84NTLGLLALSGSLLVGFVALPQPAVARR35 pKa = 11.84NNTAVGVGTGAVAGALVAGPLGAVVGGIVGGVVGSNSEE73 pKa = 3.93SARR76 pKa = 11.84PRR78 pKa = 11.84RR79 pKa = 11.84SRR81 pKa = 11.84RR82 pKa = 11.84ARR84 pKa = 11.84AVRR87 pKa = 11.84PRR89 pKa = 11.84RR90 pKa = 11.84QPAVARR96 pKa = 11.84IAPRR100 pKa = 11.84AAPAARR106 pKa = 11.84PVAQAEE112 pKa = 4.12PRR114 pKa = 11.84PVSTVPTSTTWTNPRR129 pKa = 3.63

Molecular weight: 13.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3885

0

3885

1232780

29

3564

317.3

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.17 ± 0.06

0.788 ± 0.013

5.91 ± 0.036

5.313 ± 0.04

3.321 ± 0.022

9.276 ± 0.05

1.889 ± 0.02

4.453 ± 0.028

2.532 ± 0.037

10.18 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.08 ± 0.018

2.115 ± 0.027

5.655 ± 0.043

2.678 ± 0.025

7.998 ± 0.048

4.981 ± 0.029

5.618 ± 0.039

7.897 ± 0.03

1.178 ± 0.017

1.968 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski