Hubei myriapoda virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEW9|A0A1L3KEW9_9VIRU Uncharacterized protein OS=Hubei myriapoda virus 9 OX=1922938 PE=4 SV=1
MM1 pKa = 7.52FYY3 pKa = 10.45IEE5 pKa = 5.03LLEE8 pKa = 4.33KK9 pKa = 10.88ALVTGSDD16 pKa = 3.24TGVVMGFNPFNGGYY30 pKa = 9.53KK31 pKa = 10.11HH32 pKa = 7.11FIRR35 pKa = 11.84SLPEE39 pKa = 3.61KK40 pKa = 10.57CLMLDD45 pKa = 3.18KK46 pKa = 11.16SSWDD50 pKa = 3.1WTVPTWLLSDD60 pKa = 4.62FYY62 pKa = 11.59QLLSNLGFGGCKK74 pKa = 9.49RR75 pKa = 11.84FHH77 pKa = 7.42RR78 pKa = 11.84WRR80 pKa = 11.84QLHH83 pKa = 6.43GIDD86 pKa = 4.55HH87 pKa = 6.53GFIPAMVEE95 pKa = 5.13GEE97 pKa = 4.39DD98 pKa = 3.78CDD100 pKa = 4.32LPAGIQVTGNFLTYY114 pKa = 10.13IGNSVMQLLLHH125 pKa = 6.76FMAEE129 pKa = 4.44DD130 pKa = 3.82LLHH133 pKa = 5.63EE134 pKa = 4.27QPGRR138 pKa = 11.84FYY140 pKa = 11.17CGGDD144 pKa = 3.5DD145 pKa = 5.37SIQSVPKK152 pKa = 10.64NYY154 pKa = 10.31DD155 pKa = 2.9GYY157 pKa = 11.64LEE159 pKa = 4.58FFQSVGLKK167 pKa = 9.84VRR169 pKa = 11.84GGISDD174 pKa = 2.86HH175 pKa = 6.69WEE177 pKa = 3.54FLGWKK182 pKa = 9.59FSKK185 pKa = 9.81EE186 pKa = 3.92RR187 pKa = 11.84KK188 pKa = 8.79PVPLYY193 pKa = 9.77SQKK196 pKa = 10.87HH197 pKa = 5.5LFNQQQLDD205 pKa = 3.84EE206 pKa = 5.62LIAPDD211 pKa = 4.73LLSSYY216 pKa = 10.62QLLYY220 pKa = 8.76TWEE223 pKa = 5.27PIMLNHH229 pKa = 6.92IKK231 pKa = 10.52SALSWIKK238 pKa = 10.61PEE240 pKa = 4.09FVRR243 pKa = 11.84SDD245 pKa = 2.97WYY247 pKa = 9.9LQCMWEE253 pKa = 4.19GLL255 pKa = 3.78

Molecular weight: 29.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEW5|A0A1L3KEW5_9VIRU RdRp OS=Hubei myriapoda virus 9 OX=1922938 PE=4 SV=1
MM1 pKa = 7.07SQSSNDD7 pKa = 3.09YY8 pKa = 11.0SMPKK12 pKa = 9.67VRR14 pKa = 11.84SLFDD18 pKa = 3.66LASDD22 pKa = 3.41QFLAVVALSWWLPFSLPWAIANLLLEE48 pKa = 5.2RR49 pKa = 11.84DD50 pKa = 3.86PGMPTAMLSYY60 pKa = 9.54PDD62 pKa = 4.24LVGTEE67 pKa = 4.49TKK69 pKa = 10.39RR70 pKa = 11.84KK71 pKa = 8.53PLRR74 pKa = 11.84LSTGVSRR81 pKa = 11.84SWRR84 pKa = 11.84HH85 pKa = 5.65CDD87 pKa = 2.78LCRR90 pKa = 11.84SIRR93 pKa = 11.84TNYY96 pKa = 9.43YY97 pKa = 8.66LPPRR101 pKa = 11.84FGVASCSEE109 pKa = 4.7CGCIWLSIPFHH120 pKa = 5.68GRR122 pKa = 11.84KK123 pKa = 9.31EE124 pKa = 3.88YY125 pKa = 10.53FLYY128 pKa = 10.77CKK130 pKa = 10.24VVQRR134 pKa = 11.84LLNRR138 pKa = 11.84GMQPLPLTRR147 pKa = 11.84DD148 pKa = 3.17ILRR151 pKa = 11.84RR152 pKa = 11.84PYY154 pKa = 10.53LVYY157 pKa = 9.08TGKK160 pKa = 9.76YY161 pKa = 8.87ARR163 pKa = 11.84FEE165 pKa = 4.28RR166 pKa = 11.84VVSTTILRR174 pKa = 11.84LAQMSDD180 pKa = 3.06SDD182 pKa = 3.66ICNTLWSLFSRR193 pKa = 11.84VRR195 pKa = 11.84HH196 pKa = 4.65TSAWGRR202 pKa = 11.84RR203 pKa = 11.84HH204 pKa = 6.79SGACDD209 pKa = 3.43RR210 pKa = 11.84KK211 pKa = 10.38CSVHH215 pKa = 6.66RR216 pKa = 11.84DD217 pKa = 3.24AVSFCNTGCSGCARR231 pKa = 11.84IQDD234 pKa = 3.48RR235 pKa = 11.84RR236 pKa = 11.84HH237 pKa = 5.28YY238 pKa = 10.35RR239 pKa = 11.84LSFLTNEE246 pKa = 4.54GFCLGTAAANDD257 pKa = 3.99CTAVRR262 pKa = 11.84CGTRR266 pKa = 11.84PEE268 pKa = 4.39HH269 pKa = 6.48FCTAVWRR276 pKa = 11.84SSGQAVDD283 pKa = 3.6ILWAIVASSKK293 pKa = 8.71VVRR296 pKa = 11.84IPEE299 pKa = 3.98ILWIHH304 pKa = 5.47TKK306 pKa = 10.66GGIWGTNCSWQTSCSYY322 pKa = 11.17ARR324 pKa = 11.84WLRR327 pKa = 11.84GQIQHH332 pKa = 7.17LDD334 pKa = 3.3RR335 pKa = 11.84DD336 pKa = 4.28GLHH339 pKa = 7.05LILFRR344 pKa = 11.84PAEE347 pKa = 4.11QIGLAILPEE356 pKa = 4.53DD357 pKa = 4.19PNPVEE362 pKa = 4.04VQPGIICEE370 pKa = 3.97YY371 pKa = 9.99GRR373 pKa = 11.84VSRR376 pKa = 11.84EE377 pKa = 3.66LGSKK381 pKa = 10.17RR382 pKa = 11.84KK383 pKa = 7.56EE384 pKa = 4.23TQGLARR390 pKa = 11.84YY391 pKa = 8.28GRR393 pKa = 11.84QRR395 pKa = 11.84LLPRR399 pKa = 11.84TWWQDD404 pKa = 3.4PIDD407 pKa = 4.11RR408 pKa = 11.84PIYY411 pKa = 10.83VNGCYY416 pKa = 9.76ARR418 pKa = 11.84CIGLVPHH425 pKa = 6.55RR426 pKa = 11.84TFGPSFWRR434 pKa = 11.84LGRR437 pKa = 11.84LHH439 pKa = 7.09GPLFSGVRR447 pKa = 11.84RR448 pKa = 11.84EE449 pKa = 3.99SEE451 pKa = 3.77MRR453 pKa = 11.84RR454 pKa = 11.84LRR456 pKa = 11.84DD457 pKa = 3.52RR458 pKa = 11.84IQRR461 pKa = 11.84DD462 pKa = 3.39RR463 pKa = 11.84EE464 pKa = 4.06RR465 pKa = 11.84EE466 pKa = 3.9RR467 pKa = 11.84SSLPSSAEE475 pKa = 3.89AQHH478 pKa = 6.45SEE480 pKa = 3.95NDD482 pKa = 3.57SNQIGG487 pKa = 3.38

Molecular weight: 55.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1870

255

667

467.5

52.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.471 ± 0.765

2.781 ± 0.513

4.973 ± 0.291

4.492 ± 0.379

3.797 ± 0.51

7.754 ± 0.285

2.246 ± 0.281

4.652 ± 0.187

4.439 ± 0.776

8.93 ± 0.854

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.032 ± 0.251

2.674 ± 0.473

6.364 ± 0.421

4.545 ± 0.419

6.471 ± 1.753

9.626 ± 1.089

5.294 ± 0.63

6.417 ± 0.498

2.674 ± 0.313

3.369 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski