Amino acid dipepetide frequency for Caenorhabditis brenneri (Nematode worm)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.365AlaAla: 5.365 ± 0.054	1.053AlaCys: 1.053 ± 0.011	3.093AlaAsp: 3.093 ± 0.02	4.244AlaGlu: 4.244 ± 0.034	2.593AlaPhe: 2.593 ± 0.016	3.16AlaGly: 3.16 ± 0.024	1.364AlaHis: 1.364 ± 0.012	3.652AlaIle: 3.652 ± 0.018	3.884AlaLys: 3.884 ± 0.034	5.188AlaLeu: 5.188 ± 0.025	1.611AlaMet: 1.611 ± 0.012	2.675AlaAsn: 2.675 ± 0.016	3.889AlaPro: 3.889 ± 0.052	2.638AlaGln: 2.638 ± 0.02	3.14AlaArg: 3.14 ± 0.021	4.564AlaSer: 4.564 ± 0.031	3.758AlaThr: 3.758 ± 0.023	4.154AlaVal: 4.154 ± 0.025	0.581AlaTrp: 0.581 ± 0.009	1.698AlaTyr: 1.698 ± 0.013	0.0AlaXaa: 0.0 ± 0.0
Cys	1.126CysAla: 1.126 ± 0.014	0.526CysCys: 0.526 ± 0.011	1.09CysAsp: 1.09 ± 0.015	1.168CysGlu: 1.168 ± 0.012	0.948CysPhe: 0.948 ± 0.01	1.256CysGly: 1.256 ± 0.015	0.494CysHis: 0.494 ± 0.008	1.061CysIle: 1.061 ± 0.012	1.046CysLys: 1.046 ± 0.012	1.663CysLeu: 1.663 ± 0.015	0.455CysMet: 0.455 ± 0.008	0.825CysAsn: 0.825 ± 0.011	1.062CysPro: 1.062 ± 0.015	0.853CysGln: 0.853 ± 0.014	1.049CysArg: 1.049 ± 0.011	1.602CysSer: 1.602 ± 0.017	1.025CysThr: 1.025 ± 0.012	1.173CysVal: 1.173 ± 0.013	0.219CysTrp: 0.219 ± 0.005	0.625CysTyr: 0.625 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	3.286AspAla: 3.286 ± 0.022	0.991AspCys: 0.991 ± 0.013	4.028AspAsp: 4.028 ± 0.03	4.839AspGlu: 4.839 ± 0.031	2.579AspPhe: 2.579 ± 0.015	3.424AspGly: 3.424 ± 0.044	1.19AspHis: 1.19 ± 0.011	3.066AspIle: 3.066 ± 0.017	2.919AspLys: 2.919 ± 0.019	4.483AspLeu: 4.483 ± 0.02	1.32AspMet: 1.32 ± 0.012	2.288AspAsn: 2.288 ± 0.016	2.508AspPro: 2.508 ± 0.019	2.062AspGln: 2.062 ± 0.014	2.644AspArg: 2.644 ± 0.018	4.025AspSer: 4.025 ± 0.025	2.541AspThr: 2.541 ± 0.015	3.602AspVal: 3.602 ± 0.019	0.667AspTrp: 0.667 ± 0.008	1.867AspTyr: 1.867 ± 0.015	0.0AspXaa: 0.0 ± 0.0
Glu	4.28GluAla: 4.28 ± 0.036	1.143GluCys: 1.143 ± 0.014	4.404GluAsp: 4.404 ± 0.025	7.587GluGlu: 7.587 ± 0.061	2.759GluPhe: 2.759 ± 0.017	2.907GluGly: 2.907 ± 0.024	1.539GluHis: 1.539 ± 0.018	4.136GluIle: 4.136 ± 0.023	6.36GluLys: 6.36 ± 0.052	5.69GluLeu: 5.69 ± 0.027	2.145GluMet: 2.145 ± 0.017	3.917GluAsn: 3.917 ± 0.022	2.762GluPro: 2.762 ± 0.026	3.017GluGln: 3.017 ± 0.042	3.637GluArg: 3.637 ± 0.024	4.488GluSer: 4.488 ± 0.029	3.904GluThr: 3.904 ± 0.028	4.005GluVal: 4.005 ± 0.026	0.779GluTrp: 0.779 ± 0.01	2.112GluTyr: 2.112 ± 0.017	0.0GluXaa: 0.0 ± 0.0
Phe	2.607PheAla: 2.607 ± 0.017	1.037PheCys: 1.037 ± 0.01	2.728PheAsp: 2.728 ± 0.018	3.001PheGlu: 3.001 ± 0.019	2.488PhePhe: 2.488 ± 0.017	2.837PheGly: 2.837 ± 0.018	1.141PheHis: 1.141 ± 0.012	2.701PheIle: 2.701 ± 0.017	2.412PheLys: 2.412 ± 0.018	4.322PheLeu: 4.322 ± 0.023	1.191PheMet: 1.191 ± 0.011	2.124PheAsn: 2.124 ± 0.014	1.974PhePro: 1.974 ± 0.015	1.802PheGln: 1.802 ± 0.013	2.2PheArg: 2.2 ± 0.016	3.532PheSer: 3.532 ± 0.017	2.317PheThr: 2.317 ± 0.014	3.026PheVal: 3.026 ± 0.019	0.587PheTrp: 0.587 ± 0.007	1.656PheTyr: 1.656 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	3.338GlyAla: 3.338 ± 0.027	1.064GlyCys: 1.064 ± 0.013	2.681GlyAsp: 2.681 ± 0.019	3.237GlyGlu: 3.237 ± 0.024	2.558GlyPhe: 2.558 ± 0.018	4.053GlyGly: 4.053 ± 0.05	1.253GlyHis: 1.253 ± 0.014	3.088GlyIle: 3.088 ± 0.017	3.393GlyLys: 3.393 ± 0.023	3.909GlyLeu: 3.909 ± 0.023	1.35GlyMet: 1.35 ± 0.013	2.696GlyAsn: 2.696 ± 0.025	2.375GlyPro: 2.375 ± 0.034	2.007GlyGln: 2.007 ± 0.019	2.759GlyArg: 2.759 ± 0.021	4.247GlySer: 4.247 ± 0.028	3.06GlyThr: 3.06 ± 0.025	3.297GlyVal: 3.297 ± 0.02	0.641GlyTrp: 0.641 ± 0.008	1.963GlyTyr: 1.963 ± 0.018	0.0GlyXaa: 0.0 ± 0.0
His	1.205HisAla: 1.205 ± 0.011	0.496HisCys: 0.496 ± 0.007	1.09HisAsp: 1.09 ± 0.01	1.345HisGlu: 1.345 ± 0.014	1.262HisPhe: 1.262 ± 0.012	1.298HisGly: 1.298 ± 0.013	0.943HisHis: 0.943 ± 0.02	1.277HisIle: 1.277 ± 0.012	1.153HisLys: 1.153 ± 0.009	2.201HisLeu: 2.201 ± 0.015	0.579HisMet: 0.579 ± 0.008	0.991HisAsn: 0.991 ± 0.009	1.272HisPro: 1.272 ± 0.011	1.144HisGln: 1.144 ± 0.011	1.379HisArg: 1.379 ± 0.012	1.73HisSer: 1.73 ± 0.015	1.069HisThr: 1.069 ± 0.011	1.498HisVal: 1.498 ± 0.014	0.305HisTrp: 0.305 ± 0.005	0.797HisTyr: 0.797 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.633IleAla: 3.633 ± 0.019	1.237IleCys: 1.237 ± 0.012	3.48IleAsp: 3.48 ± 0.021	4.119IleGlu: 4.119 ± 0.021	2.87IlePhe: 2.87 ± 0.02	3.303IleGly: 3.303 ± 0.021	1.408IleHis: 1.408 ± 0.013	3.379IleIle: 3.379 ± 0.021	3.192IleLys: 3.192 ± 0.018	5.274IleLeu: 5.274 ± 0.025	1.318IleMet: 1.318 ± 0.012	2.585IleAsn: 2.585 ± 0.016	3.161IlePro: 3.161 ± 0.02	2.493IleGln: 2.493 ± 0.016	3.297IleArg: 3.297 ± 0.019	4.427IleSer: 4.427 ± 0.024	3.129IleThr: 3.129 ± 0.022	3.863IleVal: 3.863 ± 0.021	0.644IleTrp: 0.644 ± 0.007	1.829IleTyr: 1.829 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.665LysAla: 3.665 ± 0.033	1.321LysCys: 1.321 ± 0.014	3.309LysAsp: 3.309 ± 0.024	5.158LysGlu: 5.158 ± 0.033	2.644LysPhe: 2.644 ± 0.017	2.526LysGly: 2.526 ± 0.016	1.349LysHis: 1.349 ± 0.012	3.923LysIle: 3.923 ± 0.022	6.487LysLys: 6.487 ± 0.043	5.664LysLeu: 5.664 ± 0.025	2.02LysMet: 2.02 ± 0.015	3.556LysAsn: 3.556 ± 0.021	2.855LysPro: 2.855 ± 0.041	2.482LysGln: 2.482 ± 0.017	3.841LysArg: 3.841 ± 0.021	4.637LysSer: 4.637 ± 0.022	4.051LysThr: 4.051 ± 0.022	3.654LysVal: 3.654 ± 0.016	0.794LysTrp: 0.794 ± 0.01	2.086LysTyr: 2.086 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	5.517LeuAla: 5.517 ± 0.026	1.597LeuCys: 1.597 ± 0.013	4.455LeuAsp: 4.455 ± 0.025	6.028LeuGlu: 6.028 ± 0.029	4.09LeuPhe: 4.09 ± 0.022	3.866LeuGly: 3.866 ± 0.023	2.031LeuHis: 2.031 ± 0.014	5.053LeuIle: 5.053 ± 0.024	5.923LeuLys: 5.923 ± 0.025	8.037LeuLeu: 8.037 ± 0.038	2.213LeuMet: 2.213 ± 0.017	4.157LeuAsn: 4.157 ± 0.02	4.377LeuPro: 4.377 ± 0.031	3.539LeuGln: 3.539 ± 0.022	4.624LeuArg: 4.624 ± 0.027	6.335LeuSer: 6.335 ± 0.035	4.666LeuThr: 4.666 ± 0.021	5.074LeuVal: 5.074 ± 0.02	0.853LeuTrp: 0.853 ± 0.01	2.388LeuTyr: 2.388 ± 0.017	0.001LeuXaa: 0.001 ± 0.0
Met	1.79MetAla: 1.79 ± 0.014	0.473MetCys: 0.473 ± 0.007	1.561MetAsp: 1.561 ± 0.012	2.036MetGlu: 2.036 ± 0.016	1.138MetPhe: 1.138 ± 0.011	1.202MetGly: 1.202 ± 0.013	0.522MetHis: 0.522 ± 0.007	1.591MetIle: 1.591 ± 0.012	1.983MetLys: 1.983 ± 0.015	2.072MetLeu: 2.072 ± 0.015	0.892MetMet: 0.892 ± 0.01	1.432MetAsn: 1.432 ± 0.012	1.179MetPro: 1.179 ± 0.012	0.967MetGln: 0.967 ± 0.011	1.402MetArg: 1.402 ± 0.01	2.112MetSer: 2.112 ± 0.015	1.595MetThr: 1.595 ± 0.013	1.431MetVal: 1.431 ± 0.012	0.261MetTrp: 0.261 ± 0.005	0.741MetTyr: 0.741 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.837AsnAla: 2.837 ± 0.018	0.981AsnCys: 0.981 ± 0.01	2.537AsnAsp: 2.537 ± 0.014	3.288AsnGlu: 3.288 ± 0.016	2.259AsnPhe: 2.259 ± 0.016	3.375AsnGly: 3.375 ± 0.024	1.142AsnHis: 1.142 ± 0.011	2.704AsnIle: 2.704 ± 0.016	2.619AsnLys: 2.619 ± 0.017	4.134AsnLeu: 4.134 ± 0.021	1.267AsnMet: 1.267 ± 0.012	2.523AsnAsn: 2.523 ± 0.018	2.429AsnPro: 2.429 ± 0.019	2.17AsnGln: 2.17 ± 0.016	2.593AsnArg: 2.593 ± 0.015	3.793AsnSer: 3.793 ± 0.023	2.481AsnThr: 2.481 ± 0.015	3.057AsnVal: 3.057 ± 0.019	0.589AsnTrp: 0.589 ± 0.007	1.656AsnTyr: 1.656 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	3.399ProAla: 3.399 ± 0.033	0.707ProCys: 0.707 ± 0.013	2.494ProAsp: 2.494 ± 0.032	3.551ProGlu: 3.551 ± 0.029	2.009ProPhe: 2.009 ± 0.017	2.795ProGly: 2.795 ± 0.046	1.003ProHis: 1.003 ± 0.011	2.997ProIle: 2.997 ± 0.022	3.076ProLys: 3.076 ± 0.023	3.737ProLeu: 3.737 ± 0.028	1.202ProMet: 1.202 ± 0.012	2.327ProAsn: 2.327 ± 0.017	4.313ProPro: 4.313 ± 0.047	2.164ProGln: 2.164 ± 0.024	2.444ProArg: 2.444 ± 0.017	4.43ProSer: 4.43 ± 0.03	3.434ProThr: 3.434 ± 0.037	3.424ProVal: 3.424 ± 0.066	0.422ProTrp: 0.422 ± 0.007	1.387ProTyr: 1.387 ± 0.012	0.0ProXaa: 0.0 ± 0.0
Gln	2.436GlnAla: 2.436 ± 0.037	0.83GlnCys: 0.83 ± 0.013	1.706GlnAsp: 1.706 ± 0.013	2.719GlnGlu: 2.719 ± 0.024	1.866GlnPhe: 1.866 ± 0.013	1.72GlnGly: 1.72 ± 0.017	1.012GlnHis: 1.012 ± 0.012	2.392GlnIle: 2.392 ± 0.016	3.16GlnLys: 3.16 ± 0.017	3.878GlnLeu: 3.878 ± 0.022	1.366GlnMet: 1.366 ± 0.014	2.312GlnAsn: 2.312 ± 0.018	2.126GlnPro: 2.126 ± 0.022	2.809GlnGln: 2.809 ± 0.045	2.269GlnArg: 2.269 ± 0.019	2.773GlnSer: 2.773 ± 0.021	2.207GlnThr: 2.207 ± 0.017	2.272GlnVal: 2.272 ± 0.017	0.498GlnTrp: 0.498 ± 0.006	1.31GlnTyr: 1.31 ± 0.012	0.0GlnXaa: 0.0 ± 0.0
Arg	3.082ArgAla: 3.082 ± 0.017	0.966ArgCys: 0.966 ± 0.013	2.704ArgAsp: 2.704 ± 0.021	3.552ArgGlu: 3.552 ± 0.024	2.354ArgPhe: 2.354 ± 0.014	2.596ArgGly: 2.596 ± 0.021	1.337ArgHis: 1.337 ± 0.011	3.242ArgIle: 3.242 ± 0.018	4.184ArgLys: 4.184 ± 0.023	4.469ArgLeu: 4.469 ± 0.022	1.449ArgMet: 1.449 ± 0.012	2.885ArgAsn: 2.885 ± 0.016	2.41ArgPro: 2.41 ± 0.019	2.299ArgGln: 2.299 ± 0.018	4.17ArgArg: 4.17 ± 0.029	3.819ArgSer: 3.819 ± 0.025	2.723ArgThr: 2.723 ± 0.017	3.02ArgVal: 3.02 ± 0.019	0.562ArgTrp: 0.562 ± 0.008	1.575ArgTyr: 1.575 ± 0.011	0.001ArgXaa: 0.001 ± 0.0
Ser	4.689SerAla: 4.689 ± 0.025	1.436SerCys: 1.436 ± 0.015	4.184SerAsp: 4.184 ± 0.023	4.912SerGlu: 4.912 ± 0.032	3.428SerPhe: 3.428 ± 0.022	4.28SerGly: 4.28 ± 0.037	1.619SerHis: 1.619 ± 0.013	4.52SerIle: 4.52 ± 0.023	4.514SerLys: 4.514 ± 0.025	6.255SerLeu: 6.255 ± 0.027	1.899SerMet: 1.899 ± 0.014	3.672SerAsn: 3.672 ± 0.02	3.934SerPro: 3.934 ± 0.028	2.997SerGln: 2.997 ± 0.024	3.923SerArg: 3.923 ± 0.024	8.473SerSer: 8.473 ± 0.062	5.491SerThr: 5.491 ± 0.048	4.592SerVal: 4.592 ± 0.019	0.781SerTrp: 0.781 ± 0.009	2.155SerTyr: 2.155 ± 0.015	0.001SerXaa: 0.001 ± 0.0
Thr	3.691ThrAla: 3.691 ± 0.024	1.195ThrCys: 1.195 ± 0.016	2.924ThrAsp: 2.924 ± 0.045	3.637ThrGlu: 3.637 ± 0.032	2.524ThrPhe: 2.524 ± 0.015	3.064ThrGly: 3.064 ± 0.023	1.16ThrHis: 1.16 ± 0.01	3.652ThrIle: 3.652 ± 0.019	3.27ThrLys: 3.27 ± 0.022	4.703ThrLeu: 4.703 ± 0.023	1.363ThrMet: 1.363 ± 0.012	2.543ThrAsn: 2.543 ± 0.016	3.556ThrPro: 3.556 ± 0.027	1.959ThrGln: 1.959 ± 0.016	2.67ThrArg: 2.67 ± 0.017	5.135ThrSer: 5.135 ± 0.038	4.817ThrThr: 4.817 ± 0.074	4.066ThrVal: 4.066 ± 0.025	0.634ThrTrp: 0.634 ± 0.008	1.604ThrTyr: 1.604 ± 0.01	0.001ThrXaa: 0.001 ± 0.0
Val	4.094ValAla: 4.094 ± 0.021	1.25ValCys: 1.25 ± 0.014	3.5ValAsp: 3.5 ± 0.019	4.528ValGlu: 4.528 ± 0.069	3.114ValPhe: 3.114 ± 0.016	2.934ValGly: 2.934 ± 0.02	1.432ValHis: 1.432 ± 0.012	3.684ValIle: 3.684 ± 0.02	3.8ValLys: 3.8 ± 0.023	5.419ValLeu: 5.419 ± 0.024	1.561ValMet: 1.561 ± 0.012	2.786ValAsn: 2.786 ± 0.016	3.296ValPro: 3.296 ± 0.026	2.486ValGln: 2.486 ± 0.016	2.974ValArg: 2.974 ± 0.019	4.499ValSer: 4.499 ± 0.022	3.584ValThr: 3.584 ± 0.024	4.264ValVal: 4.264 ± 0.023	0.675ValTrp: 0.675 ± 0.008	1.965ValTyr: 1.965 ± 0.015	0.0ValXaa: 0.0 ± 0.0
Trp	0.578TrpAla: 0.578 ± 0.007	0.222TrpCys: 0.222 ± 0.005	0.576TrpAsp: 0.576 ± 0.007	0.634TrpGlu: 0.634 ± 0.008	0.551TrpPhe: 0.551 ± 0.007	0.454TrpGly: 0.454 ± 0.007	0.258TrpHis: 0.258 ± 0.005	0.806TrpIle: 0.806 ± 0.009	0.957TrpLys: 0.957 ± 0.01	0.969TrpLeu: 0.969 ± 0.011	0.381TrpMet: 0.381 ± 0.005	0.679TrpAsn: 0.679 ± 0.008	0.41TrpPro: 0.41 ± 0.007	0.427TrpGln: 0.427 ± 0.006	0.651TrpArg: 0.651 ± 0.007	0.783TrpSer: 0.783 ± 0.009	0.674TrpThr: 0.674 ± 0.008	0.564TrpVal: 0.564 ± 0.008	0.159TrpTrp: 0.159 ± 0.003	0.353TrpTyr: 0.353 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.692TyrAla: 1.692 ± 0.013	0.74TyrCys: 0.74 ± 0.009	1.782TyrAsp: 1.782 ± 0.012	1.986TyrGlu: 1.986 ± 0.015	1.65TyrPhe: 1.65 ± 0.013	1.921TyrGly: 1.921 ± 0.016	0.838TyrHis: 0.838 ± 0.008	1.715TyrIle: 1.715 ± 0.014	1.69TyrLys: 1.69 ± 0.013	2.758TyrLeu: 2.758 ± 0.017	0.798TyrMet: 0.798 ± 0.009	1.457TyrAsn: 1.457 ± 0.013	1.425TyrPro: 1.425 ± 0.018	1.347TyrGln: 1.347 ± 0.012	1.731TyrArg: 1.731 ± 0.013	2.35TyrSer: 2.35 ± 0.014	1.641TyrThr: 1.641 ± 0.012	1.812TyrVal: 1.812 ± 0.015	0.433TyrTrp: 0.433 ± 0.006	1.215TyrTyr: 1.215 ± 0.011	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.327XaaXaa: 0.327 ± 0.109

Statistics based on 29982 proteins (11989625 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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