Shigella phage SfV (Shigella flexneri bacteriophage V) (Bacteriophage SfV)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8SBH5|Q8SBH5_BPSF5 Uncharacterized protein OS=Shigella phage SfV OX=55884 GN=orf8 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 8.86LTPVIAALRR11 pKa = 11.84ARR13 pKa = 11.84CPYY16 pKa = 10.06FEE18 pKa = 4.39NRR20 pKa = 11.84VAGAAQFKK28 pKa = 10.19NLPEE32 pKa = 4.11VGKK35 pKa = 10.51LRR37 pKa = 11.84LPAAYY42 pKa = 9.22VVPGDD47 pKa = 4.84DD48 pKa = 4.34SPGEE52 pKa = 4.13NKK54 pKa = 10.35SQTDD58 pKa = 3.3YY59 pKa = 10.55WQEE62 pKa = 3.75LKK64 pKa = 10.91EE65 pKa = 4.23GFSVVVILSNGRR77 pKa = 11.84DD78 pKa = 3.43EE79 pKa = 5.21RR80 pKa = 11.84GQFASYY86 pKa = 11.14DD87 pKa = 3.57VVDD90 pKa = 5.36DD91 pKa = 3.79VRR93 pKa = 11.84QMLFKK98 pKa = 10.84ALLGWNPEE106 pKa = 3.8ACGNPITYY114 pKa = 10.35DD115 pKa = 3.18GGTLLDD121 pKa = 4.22LNRR124 pKa = 11.84HH125 pKa = 4.61EE126 pKa = 6.12LIYY129 pKa = 10.88QFDD132 pKa = 3.96FSVISEE138 pKa = 4.21LTEE141 pKa = 4.82DD142 pKa = 3.97DD143 pKa = 3.6TRR145 pKa = 11.84QQDD148 pKa = 3.75EE149 pKa = 4.77LNSLDD154 pKa = 4.01EE155 pKa = 4.6LRR157 pKa = 11.84TLAIDD162 pKa = 4.15VDD164 pKa = 4.29YY165 pKa = 10.82LDD167 pKa = 5.29PGNGPDD173 pKa = 4.82GDD175 pKa = 4.3IEE177 pKa = 4.32HH178 pKa = 6.62HH179 pKa = 6.01TEE181 pKa = 3.25ITLPSS186 pKa = 3.42

Molecular weight: 20.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8SBF3|Q8SBF3_BPSF5 Immunity region OS=Shigella phage SfV OX=55884 GN=orf37 PE=4 SV=1
MM1 pKa = 7.71LNPLSLNIRR10 pKa = 11.84RR11 pKa = 11.84LLQRR15 pKa = 11.84KK16 pKa = 6.73KK17 pKa = 9.37TSIPTVGQWYY27 pKa = 5.61TTPAGHH33 pKa = 6.23VLRR36 pKa = 11.84VSLVDD41 pKa = 3.87RR42 pKa = 11.84EE43 pKa = 4.3CQKK46 pKa = 10.88VICEE50 pKa = 4.01PLGRR54 pKa = 11.84NYY56 pKa = 9.75RR57 pKa = 11.84VSMPLITFRR66 pKa = 11.84SEE68 pKa = 3.73KK69 pKa = 10.37NMKK72 pKa = 9.81HH73 pKa = 6.17LGGAAA78 pKa = 3.13

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11614

56

611

219.1

24.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.299 ± 0.332

1.343 ± 0.159

5.614 ± 0.281

6.096 ± 0.342

3.642 ± 0.256

7.164 ± 0.331

1.937 ± 0.184

5.433 ± 0.343

5.252 ± 0.307

8.722 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.686 ± 0.196

4.176 ± 0.153

4.675 ± 0.198

3.909 ± 0.187

6.415 ± 0.384

6.113 ± 0.22

5.872 ± 0.344

6.794 ± 0.262

1.894 ± 0.174

2.962 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski