Geoalkalibacter ferrihydriticus DSM 17813

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae; Geoalkalibacter; Geoalkalibacter ferrihydriticus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C2HTE0|A0A0C2HTE0_9DELT Histidine kinase OS=Geoalkalibacter ferrihydriticus DSM 17813 OX=1121915 GN=GFER_12560 PE=4 SV=1
MM1 pKa = 7.32AVVFPPEE8 pKa = 4.2NVSSPFSDD16 pKa = 3.77LDD18 pKa = 3.36MVATVFEE25 pKa = 4.47MAPGSKK31 pKa = 10.18DD32 pKa = 3.52YY33 pKa = 9.98ITSEE37 pKa = 4.54FPNSTNFAYY46 pKa = 9.56IYY48 pKa = 10.59RR49 pKa = 11.84DD50 pKa = 3.39LTLDD54 pKa = 3.54AGQQFTLAWNYY65 pKa = 10.0VATDD69 pKa = 3.38YY70 pKa = 11.84APFNDD75 pKa = 3.31ASFVSFVNTSNPSDD89 pKa = 3.67DD90 pKa = 3.21TSRR93 pKa = 11.84IYY95 pKa = 11.01GLRR98 pKa = 11.84EE99 pKa = 3.34QVMILGATVTGTGNWSTGDD118 pKa = 3.37GGSTGWQFATFRR130 pKa = 11.84AGEE133 pKa = 4.15AGTYY137 pKa = 10.08RR138 pKa = 11.84LGFAAFNLDD147 pKa = 3.37DD148 pKa = 4.31TVFSPYY154 pKa = 10.91LFLDD158 pKa = 3.74NPAGEE163 pKa = 4.3TLKK166 pKa = 11.0NGQAFDD172 pKa = 4.93SIPPDD177 pKa = 3.36PTGPLGDD184 pKa = 3.66IFHH187 pKa = 6.82TVTPSAGVGGSISPSSAQTVGDD209 pKa = 3.69GATAQFTLLPQPGYY223 pKa = 10.69SIASVGGTCGGSLDD237 pKa = 3.85GNTYY241 pKa = 5.95TTSAISSGCTVVATFTATTYY261 pKa = 10.87SVSFDD266 pKa = 3.55LAGGSRR272 pKa = 11.84TGGGEE277 pKa = 3.89LSQTVTHH284 pKa = 6.47GQAAAAPEE292 pKa = 4.23LSAPAGKK299 pKa = 8.23TFTGWDD305 pKa = 3.29NSFDD309 pKa = 3.46IVTADD314 pKa = 4.07LSITAQYY321 pKa = 11.44VDD323 pKa = 3.36TTYY326 pKa = 11.23SVSFDD331 pKa = 3.04XFDD334 pKa = 4.04IVTADD339 pKa = 3.81LSITAQYY346 pKa = 11.44VDD348 pKa = 3.36TTYY351 pKa = 11.27SVSFDD356 pKa = 3.55LAGGSRR362 pKa = 11.84TGGGEE367 pKa = 3.89LSQTVTHH374 pKa = 6.47GQAAAAPEE382 pKa = 4.23LSAPAGKK389 pKa = 8.23TFTGWDD395 pKa = 3.29NGFDD399 pKa = 3.31IVTADD404 pKa = 4.1LSITAQYY411 pKa = 11.44VDD413 pKa = 3.36TTYY416 pKa = 11.27SVSFDD421 pKa = 3.55LAGGSRR427 pKa = 11.84TGGGASILSRR437 pKa = 11.84RR438 pKa = 11.84ISASPRR444 pKa = 11.84SMSTPPTASASIWPAARR461 pKa = 11.84APAAVSS467 pKa = 3.26

Molecular weight: 48.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C2HYE8|A0A0C2HYE8_9DELT Uncharacterized protein OS=Geoalkalibacter ferrihydriticus DSM 17813 OX=1121915 GN=GFER_03745 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.94RR4 pKa = 11.84TYY6 pKa = 9.06QPSRR10 pKa = 11.84IRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 8.37RR15 pKa = 11.84THH17 pKa = 6.12GFRR20 pKa = 11.84KK21 pKa = 9.99RR22 pKa = 11.84MLTSNGQQVIKK33 pKa = 10.43RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.21GRR40 pKa = 11.84HH41 pKa = 4.66RR42 pKa = 11.84LVVEE46 pKa = 4.28IPKK49 pKa = 10.35KK50 pKa = 10.48

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2928

0

2928

946041

37

2847

323.1

35.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.115 ± 0.057

1.207 ± 0.021

5.439 ± 0.035

6.748 ± 0.046

4.131 ± 0.032

8.085 ± 0.04

2.19 ± 0.022

5.405 ± 0.039

3.73 ± 0.038

11.299 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.022

2.951 ± 0.028

4.727 ± 0.028

3.732 ± 0.029

7.129 ± 0.053

5.242 ± 0.035

4.745 ± 0.031

7.248 ± 0.037

1.1 ± 0.019

2.512 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski