Polaribacter glomeratus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3368 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S7WFZ6|A0A2S7WFZ6_9FLAO General secretion pathway protein GspG OS=Polaribacter glomeratus OX=102 GN=BTO16_11580 PE=4 SV=1
MM1 pKa = 7.32NKK3 pKa = 10.21KK4 pKa = 8.05ITLVFVSFLFAISTTFGNNDD24 pKa = 2.75NFFFEE29 pKa = 4.62NDD31 pKa = 2.57IFTVVKK37 pKa = 10.81NDD39 pKa = 3.39FKK41 pKa = 11.63SNQKK45 pKa = 8.35TVSDD49 pKa = 4.67FAAVTTTSPIISGFSSLEE67 pKa = 3.69QYY69 pKa = 11.15NNSSYY74 pKa = 10.75YY75 pKa = 10.06ISDD78 pKa = 3.54IAYY81 pKa = 7.69TWDD84 pKa = 3.49DD85 pKa = 4.68AIIATNAVGGHH96 pKa = 6.47LAVITTTAEE105 pKa = 3.59NDD107 pKa = 3.66HH108 pKa = 6.94IFAQMQAQGNQDD120 pKa = 3.38NLLIGAFEE128 pKa = 3.97SSEE131 pKa = 4.95GIFEE135 pKa = 4.46WVNGEE140 pKa = 4.38PYY142 pKa = 10.12TNNIGGAVDD151 pKa = 4.8NIPAGLNSFIVRR163 pKa = 11.84NDD165 pKa = 2.9NGGWMDD171 pKa = 3.37YY172 pKa = 10.05FGPGSSVTIKK182 pKa = 10.37IIMEE186 pKa = 4.02VPIPDD191 pKa = 3.99TIKK194 pKa = 10.75PVITLSGANPQIIEE208 pKa = 3.91VGTNYY213 pKa = 10.31TEE215 pKa = 4.74LGATATDD222 pKa = 3.68NVDD225 pKa = 3.22AANTLLVVSTVNNQDD240 pKa = 3.15TGVVGSFTVDD250 pKa = 3.28YY251 pKa = 9.35TVSDD255 pKa = 3.95VATNAAIIVTRR266 pKa = 11.84TVNVVDD272 pKa = 3.5VTKK275 pKa = 10.46PVITLAGSNPQTIEE289 pKa = 3.89VFSVYY294 pKa = 10.85DD295 pKa = 3.97DD296 pKa = 3.61LTGAIATDD304 pKa = 3.51NYY306 pKa = 10.55DD307 pKa = 4.05DD308 pKa = 4.94NVALTANIQVISNVNSSLVGTYY330 pKa = 7.4TATYY334 pKa = 9.7NVVDD338 pKa = 4.2SEE340 pKa = 5.23SNVADD345 pKa = 4.92EE346 pKa = 4.21ITKK349 pKa = 9.02TVHH352 pKa = 6.33VVDD355 pKa = 3.88TTLPEE360 pKa = 3.94IFLVGLNPQIVEE372 pKa = 3.63IGKK375 pKa = 10.4GYY377 pKa = 10.89NEE379 pKa = 4.85LGATATDD386 pKa = 3.86NYY388 pKa = 10.98DD389 pKa = 3.62PDD391 pKa = 3.72SALISNITNTSNVNTATAGSYY412 pKa = 9.72LVSYY416 pKa = 10.73NVTDD420 pKa = 4.18ANSNTAITVEE430 pKa = 4.18RR431 pKa = 11.84IVIVKK436 pKa = 10.5SNN438 pKa = 3.03

Molecular weight: 46.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S7WK21|A0A2S7WK21_9FLAO Sulfurase OS=Polaribacter glomeratus OX=102 GN=BTO16_15980 PE=4 SV=1
MM1 pKa = 6.99QRR3 pKa = 11.84VLQRR7 pKa = 11.84VLQRR11 pKa = 11.84VLQRR15 pKa = 11.84VLQVVLLATKK25 pKa = 9.61PQGLSKK31 pKa = 11.03LIVTNIAILGIVTLLQIVLLVIKK54 pKa = 10.74LMGLNNLITSNTAIQASLSKK74 pKa = 10.78SSFMM78 pKa = 5.47

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3368

0

3368

1169193

38

2942

347.1

39.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.169 ± 0.037

0.691 ± 0.013

5.347 ± 0.036

6.471 ± 0.042

5.588 ± 0.036

6.033 ± 0.044

1.602 ± 0.018

8.54 ± 0.04

8.588 ± 0.055

9.241 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.015 ± 0.02

6.715 ± 0.047

3.041 ± 0.021

3.234 ± 0.02

3.07 ± 0.022

6.647 ± 0.033

6.032 ± 0.042

6.027 ± 0.034

1.009 ± 0.015

3.939 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski